Generation, characterization, and validation of two human induced pluripotent stem cell lines from the peripheral blood of young and older adults
Aging is a leading risk factor for the development of age-related diseases. However, how aging impacts human induced pluripotent stem cell (hiPSC) reprogramming, age-related epigenetic memory post-reprogramming, differentiation, and its potential applicability to cardiovascular regenerative medicine...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-03-01
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| Series: | Stem Cell Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1873506125000200 |
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| author | Dongli Yang Jun Chen Jerry H. Juratli Andre Monteiro da Rocha Allison Schley Nadia R. Sutton |
| author_facet | Dongli Yang Jun Chen Jerry H. Juratli Andre Monteiro da Rocha Allison Schley Nadia R. Sutton |
| author_sort | Dongli Yang |
| collection | DOAJ |
| description | Aging is a leading risk factor for the development of age-related diseases. However, how aging impacts human induced pluripotent stem cell (hiPSC) reprogramming, age-related epigenetic memory post-reprogramming, differentiation, and its potential applicability to cardiovascular regenerative medicine remains underexplored. We generated, characterized, and validated two hiPSC lines from human peripheral blood mononuclear cells (PBMCs) obtained from whole blood of young and older human donors. The two hiPSC lines expressed four pluripotency markers, have normal karyotypes and trilineage differentiation potential, and genetically match parental PBMCs. These lines are invaluable for regenerative medicine and exploring epigenetic-related molecular mechanisms that underlie aging and aging-related diseases. |
| format | Article |
| id | doaj-art-1e40cbdf096343afa9943eba2067db30 |
| institution | DOAJ |
| issn | 1873-5061 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Stem Cell Research |
| spelling | doaj-art-1e40cbdf096343afa9943eba2067db302025-08-20T03:11:46ZengElsevierStem Cell Research1873-50612025-03-018310367010.1016/j.scr.2025.103670Generation, characterization, and validation of two human induced pluripotent stem cell lines from the peripheral blood of young and older adultsDongli Yang0Jun Chen1Jerry H. Juratli2Andre Monteiro da Rocha3Allison Schley4Nadia R. Sutton5Department of Medicine, Division of Cardiovascular Medicine, Vanderbilt University Medical Center Nashville TN USADepartment of Medicine, Division of Cardiovascular Medicine, Vanderbilt University Medical Center Nashville TN USADepartment of Internal Medicine, University of Michigan Ann Arbor MI USADepartment of Internal Medicine, University of Michigan Ann Arbor MI USADepartment of Internal Medicine, University of Michigan Ann Arbor MI USADepartment of Medicine, Division of Cardiovascular Medicine, Vanderbilt University Medical Center Nashville TN USA; Department of Biomedical Engineering, Vanderbilt University Nashville TN USA; Corresponding author at: Division of Cardiovascular Medicine, Vanderbilt University Medical Center, Preston Research Building 360, 2220 Pierce Ave., Nashville, TN 37232, USA.Aging is a leading risk factor for the development of age-related diseases. However, how aging impacts human induced pluripotent stem cell (hiPSC) reprogramming, age-related epigenetic memory post-reprogramming, differentiation, and its potential applicability to cardiovascular regenerative medicine remains underexplored. We generated, characterized, and validated two hiPSC lines from human peripheral blood mononuclear cells (PBMCs) obtained from whole blood of young and older human donors. The two hiPSC lines expressed four pluripotency markers, have normal karyotypes and trilineage differentiation potential, and genetically match parental PBMCs. These lines are invaluable for regenerative medicine and exploring epigenetic-related molecular mechanisms that underlie aging and aging-related diseases.http://www.sciencedirect.com/science/article/pii/S1873506125000200AgingStem cellEpigeneticHuman induced pluripotent stem cell |
| spellingShingle | Dongli Yang Jun Chen Jerry H. Juratli Andre Monteiro da Rocha Allison Schley Nadia R. Sutton Generation, characterization, and validation of two human induced pluripotent stem cell lines from the peripheral blood of young and older adults Stem Cell Research Aging Stem cell Epigenetic Human induced pluripotent stem cell |
| title | Generation, characterization, and validation of two human induced pluripotent stem cell lines from the peripheral blood of young and older adults |
| title_full | Generation, characterization, and validation of two human induced pluripotent stem cell lines from the peripheral blood of young and older adults |
| title_fullStr | Generation, characterization, and validation of two human induced pluripotent stem cell lines from the peripheral blood of young and older adults |
| title_full_unstemmed | Generation, characterization, and validation of two human induced pluripotent stem cell lines from the peripheral blood of young and older adults |
| title_short | Generation, characterization, and validation of two human induced pluripotent stem cell lines from the peripheral blood of young and older adults |
| title_sort | generation characterization and validation of two human induced pluripotent stem cell lines from the peripheral blood of young and older adults |
| topic | Aging Stem cell Epigenetic Human induced pluripotent stem cell |
| url | http://www.sciencedirect.com/science/article/pii/S1873506125000200 |
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