Antihyperglycemic Activity of <i>Alchemilla viridiflora</i> Herb Methanol Extract in Streptozotocin-Induced Diabetic Male Rats
Based on the traditional use of <i>Alchemilla</i> L. species for the treatment of diabetes, the effect of the methanol extract of <i>Alchemilla viridiflora</i> (AVM) on enzyme activity <i>in vitro</i> and its impact on blood glucose levels <i>in vivo</i&g...
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MDPI AG
2025-06-01
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| author | Jelena S. Radović Selgrad Dušan J. Ušjak Marina T. Milenković Neda Lj. Milinković Radmila M. Janković Jovan B. Jevtić Ksenija S. Mileski Marjan S. Niketić Tatjana D. Kundaković-Vasović |
| author_facet | Jelena S. Radović Selgrad Dušan J. Ušjak Marina T. Milenković Neda Lj. Milinković Radmila M. Janković Jovan B. Jevtić Ksenija S. Mileski Marjan S. Niketić Tatjana D. Kundaković-Vasović |
| author_sort | Jelena S. Radović Selgrad |
| collection | DOAJ |
| description | Based on the traditional use of <i>Alchemilla</i> L. species for the treatment of diabetes, the effect of the methanol extract of <i>Alchemilla viridiflora</i> (AVM) on enzyme activity <i>in vitro</i> and its impact on blood glucose levels <i>in vivo</i> were investigated. Diabetes was induced in male Sprague-Dawley rats using streptozotocin. AVM was administered to both normal and STZ-diabetic rats for 20 days at three different doses. Blood glucose levels and body weights of the treated animals were monitored throughout the experiment. After 20 days, serum insulin, cholesterol, triglycerides, and high- and low-density lipoproteins were measured. In addition, a histological analysis of the pancreas was performed. The AVM demonstrated inhibitory effects on the activities of all tested enzymes. In the <i>in vivo</i> experiment, a statistically significant reduction in body weight was observed in the AVM-treated animals at all three doses compared with the normal control group. Notably, a dose of 200 mg/kg significantly decreased blood glucose levels on both the 10th day and 20th day (<i>p</i> < 0.05). However, the extract showed no statistically significant effects on the tested biochemical parameters. Overall, the results of this study suggest that AVM has potential for the treatment of hyperglycemia associated with diabetes and obesity. |
| format | Article |
| id | doaj-art-1e3611e2f78c470bbd7c76641df37fac |
| institution | DOAJ |
| issn | 1420-3049 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj-art-1e3611e2f78c470bbd7c76641df37fac2025-08-20T03:16:43ZengMDPI AGMolecules1420-30492025-06-013013281910.3390/molecules30132819Antihyperglycemic Activity of <i>Alchemilla viridiflora</i> Herb Methanol Extract in Streptozotocin-Induced Diabetic Male RatsJelena S. Radović Selgrad0Dušan J. Ušjak1Marina T. Milenković2Neda Lj. Milinković3Radmila M. Janković4Jovan B. Jevtić5Ksenija S. Mileski6Marjan S. Niketić7Tatjana D. Kundaković-Vasović8Department of Pharmacognosy, Faculty of Pharmacy, University of Belgrade, Vojvode Stepe 450, 11221 Belgrade, SerbiaDepartment of Microbiology and Immunology, Faculty of Pharmacy, University of Belgrade, Vojvode Stepe 450, 11221 Belgrade, SerbiaDepartment of Microbiology and Immunology, Faculty of Pharmacy, University of Belgrade, Vojvode Stepe 450, 11221 Belgrade, SerbiaDepartment of Medical Biochemistry, Faculty of Pharmacy, University of Belgrade, Vojvode Stepe 450, 11221 Belgrade, SerbiaInstitute of Pathology “Prof. dr Đorđe Joannović”, Faculty of Medicine, University of Belgrade, dr Subotića starijeg 1, 11000 Belgrade, SerbiaInstitute of Pathology “Prof. dr Đorđe Joannović”, Faculty of Medicine, University of Belgrade, dr Subotića starijeg 1, 11000 Belgrade, SerbiaInstitute of Botany and Botanical Garden “Jevremovac”, Faculty of Biology, University of Belgrade, Takovska 43, 11000 Belgrade, SerbiaNatural History Museum, Njegoševa 51, 11111 Belgrade, SerbiaDepartment of Pharmacognosy, Faculty of Pharmacy, University of Belgrade, Vojvode Stepe 450, 11221 Belgrade, SerbiaBased on the traditional use of <i>Alchemilla</i> L. species for the treatment of diabetes, the effect of the methanol extract of <i>Alchemilla viridiflora</i> (AVM) on enzyme activity <i>in vitro</i> and its impact on blood glucose levels <i>in vivo</i> were investigated. Diabetes was induced in male Sprague-Dawley rats using streptozotocin. AVM was administered to both normal and STZ-diabetic rats for 20 days at three different doses. Blood glucose levels and body weights of the treated animals were monitored throughout the experiment. After 20 days, serum insulin, cholesterol, triglycerides, and high- and low-density lipoproteins were measured. In addition, a histological analysis of the pancreas was performed. The AVM demonstrated inhibitory effects on the activities of all tested enzymes. In the <i>in vivo</i> experiment, a statistically significant reduction in body weight was observed in the AVM-treated animals at all three doses compared with the normal control group. Notably, a dose of 200 mg/kg significantly decreased blood glucose levels on both the 10th day and 20th day (<i>p</i> < 0.05). However, the extract showed no statistically significant effects on the tested biochemical parameters. Overall, the results of this study suggest that AVM has potential for the treatment of hyperglycemia associated with diabetes and obesity.https://www.mdpi.com/1420-3049/30/13/2819<i>Alchemilla viridiflora</i>antidiabetic activityhypolipidemic activityα-amylaseα-glucosidaselipase |
| spellingShingle | Jelena S. Radović Selgrad Dušan J. Ušjak Marina T. Milenković Neda Lj. Milinković Radmila M. Janković Jovan B. Jevtić Ksenija S. Mileski Marjan S. Niketić Tatjana D. Kundaković-Vasović Antihyperglycemic Activity of <i>Alchemilla viridiflora</i> Herb Methanol Extract in Streptozotocin-Induced Diabetic Male Rats Molecules <i>Alchemilla viridiflora</i> antidiabetic activity hypolipidemic activity α-amylase α-glucosidase lipase |
| title | Antihyperglycemic Activity of <i>Alchemilla viridiflora</i> Herb Methanol Extract in Streptozotocin-Induced Diabetic Male Rats |
| title_full | Antihyperglycemic Activity of <i>Alchemilla viridiflora</i> Herb Methanol Extract in Streptozotocin-Induced Diabetic Male Rats |
| title_fullStr | Antihyperglycemic Activity of <i>Alchemilla viridiflora</i> Herb Methanol Extract in Streptozotocin-Induced Diabetic Male Rats |
| title_full_unstemmed | Antihyperglycemic Activity of <i>Alchemilla viridiflora</i> Herb Methanol Extract in Streptozotocin-Induced Diabetic Male Rats |
| title_short | Antihyperglycemic Activity of <i>Alchemilla viridiflora</i> Herb Methanol Extract in Streptozotocin-Induced Diabetic Male Rats |
| title_sort | antihyperglycemic activity of i alchemilla viridiflora i herb methanol extract in streptozotocin induced diabetic male rats |
| topic | <i>Alchemilla viridiflora</i> antidiabetic activity hypolipidemic activity α-amylase α-glucosidase lipase |
| url | https://www.mdpi.com/1420-3049/30/13/2819 |
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