Differential expression of circulating microRNAs in lactating Holstein and Jersey cows exposed to heat stress
Background: South Korea has recently faced record-high temperatures, which have adversely affected dairy production. Holstein cows, the primary dairy breed globally, are particularly sensitive to heat stress. In contrast, Jersey cows have shown greater heat tolerance, as demonstrated by phenotypic s...
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| Language: | English |
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The Korean Society of Animal Reproduction and Biotechnology
2024-12-01
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| Series: | Journal of Animal Reproduction and Biotechnology |
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| Online Access: | https://www.e-jarb.org/journal/view.html?uid=2708&vmd=Full |
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| author | Jihwan Lee Doosan Kim Byeonghwi Lim Gyeonglim Ryu Hyeonguk Baek Joohwan Kim Seungmin Ha Sangbum Kim Seunghwan Lee Taejeong Choi Inchul Choi |
| author_facet | Jihwan Lee Doosan Kim Byeonghwi Lim Gyeonglim Ryu Hyeonguk Baek Joohwan Kim Seungmin Ha Sangbum Kim Seunghwan Lee Taejeong Choi Inchul Choi |
| author_sort | Jihwan Lee |
| collection | DOAJ |
| description | Background: South Korea has recently faced record-high temperatures, which have adversely affected dairy production. Holstein cows, the primary dairy breed globally, are particularly sensitive to heat stress. In contrast, Jersey cows have shown greater heat tolerance, as demonstrated by phenotypic studies.
Methods: We investigated physiological and molecular responses to heat stress in Holstein and Jersey cows by measuring rectal temperature, milk yield, and average daily gain, confirming Holstein cows’ greater vulnerability. To explore molecular mechanisms, we analyzed circulating microRNA profiles from whole blood samples collected under heat stress and normal conditions using microRNA-sequencing. Differential expression patterns were compared between the two breeds to identify biological pathways associated with heat stress.
Results: Four microRNAs (bta-miR-20b, bta-miR-1246, bta-miR-2284x, and bta-miR- 2284y) were significantly differentially expressed in both breeds under heat stress (|FC| ≥ 2, p < 0.05). Notably, bta-miR-20b and bta-miR-1246 were linked to corpus luteum function and progesterone biosynthesis, while bta-miR-2284x and bta-miR- 2284y were associated with immune responses. A comparison of 11 potential heat stress-related microRNAs identified in previous studies of Holstein cows revealed consistent expression trends in Jersey cows, albeit with lower fold changes, suggesting their superior heat resilience.
Conclusions: Our study highlights the physiological and microRNA-based differences in heat stress responses between Holstein and Jersey cows. Jersey cows exhibited greater resilience, supported by more stable microRNA expression profiles and improved heat stress indicators, making them a promising breed for dairy production in increasingly hot climates. |
| format | Article |
| id | doaj-art-1e2ef5a4eabe453b87fee904559e9cb9 |
| institution | Kabale University |
| issn | 2671-4639 2671-4663 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | The Korean Society of Animal Reproduction and Biotechnology |
| record_format | Article |
| series | Journal of Animal Reproduction and Biotechnology |
| spelling | doaj-art-1e2ef5a4eabe453b87fee904559e9cb92025-01-14T14:54:00ZengThe Korean Society of Animal Reproduction and BiotechnologyJournal of Animal Reproduction and Biotechnology2671-46392671-46632024-12-0139422123210.12750/JARB.39.4.221Differential expression of circulating microRNAs in lactating Holstein and Jersey cows exposed to heat stressJihwan Lee0https://orcid.org/0000-0002-0040-3104Doosan Kim1https://orcid.org/0000-0002-2528-5386Byeonghwi Lim2https://orcid.org/0000-0001-8489-0044Gyeonglim Ryu3https://orcid.org/0000-0002-5903-5705Hyeonguk Baek4https://orcid.org/0009-0007-6761-5711Joohwan Kim5https://orcid.org/0009-0009-9181-3763Seungmin Ha6https://orcid.org/0000-0002-5152-1979Sangbum Kim7https://orcid.org/0000-0002-8187-4134Seunghwan Lee8https://orcid.org/0000-0003-1508-4887Taejeong Choi9https://orcid.org/0000-0002-8299-9342Inchul Choi10https://orcid.org/0000-0001-5011-2658Dairy Science Division, National Institute of Animal Science, Rural Development Administration, Cheonan 31000, KoreaDairy Science Division, National Institute of Animal Science, Rural Development Administration, Cheonan 31000, KoreaDepartment of Animal Science and Technology, Chung-Ang University, Anseong 17546, KoreaDairy Science Division, National Institute of Animal Science, Rural Development Administration, Cheonan 31000, KoreaDairy Science Division, National Institute of Animal Science, Rural Development Administration, Cheonan 31000, KoreaDairy Biotechnology R&D Center, Seoul Milk Cooperation, Yangpyeong 12528, KoreaAnimal Genetic Resources Research Center, National Institute of Animal Science, Rural Development Administration, Hamyang 50000, KoreaDairy Science Division, National Institute of Animal Science, Rural Development Administration, Cheonan 31000, KoreaDivision of Animal and Dairy Science, College of Agriculture and Life Sciences, Chungnam National University, Daejeon 34134, KoreaDairy Science Division, National Institute of Animal Science, Rural Development Administration, Cheonan 31000, KoreaDivision of Animal and Dairy Science, College of Agriculture and Life Sciences, Chungnam National University, Daejeon 34134, KoreaBackground: South Korea has recently faced record-high temperatures, which have adversely affected dairy production. Holstein cows, the primary dairy breed globally, are particularly sensitive to heat stress. In contrast, Jersey cows have shown greater heat tolerance, as demonstrated by phenotypic studies. Methods: We investigated physiological and molecular responses to heat stress in Holstein and Jersey cows by measuring rectal temperature, milk yield, and average daily gain, confirming Holstein cows’ greater vulnerability. To explore molecular mechanisms, we analyzed circulating microRNA profiles from whole blood samples collected under heat stress and normal conditions using microRNA-sequencing. Differential expression patterns were compared between the two breeds to identify biological pathways associated with heat stress. Results: Four microRNAs (bta-miR-20b, bta-miR-1246, bta-miR-2284x, and bta-miR- 2284y) were significantly differentially expressed in both breeds under heat stress (|FC| ≥ 2, p < 0.05). Notably, bta-miR-20b and bta-miR-1246 were linked to corpus luteum function and progesterone biosynthesis, while bta-miR-2284x and bta-miR- 2284y were associated with immune responses. A comparison of 11 potential heat stress-related microRNAs identified in previous studies of Holstein cows revealed consistent expression trends in Jersey cows, albeit with lower fold changes, suggesting their superior heat resilience. Conclusions: Our study highlights the physiological and microRNA-based differences in heat stress responses between Holstein and Jersey cows. Jersey cows exhibited greater resilience, supported by more stable microRNA expression profiles and improved heat stress indicators, making them a promising breed for dairy production in increasingly hot climates.https://www.e-jarb.org/journal/view.html?uid=2708&vmd=Fullcirculating micrornaheat stressholsteinjerseylactation |
| spellingShingle | Jihwan Lee Doosan Kim Byeonghwi Lim Gyeonglim Ryu Hyeonguk Baek Joohwan Kim Seungmin Ha Sangbum Kim Seunghwan Lee Taejeong Choi Inchul Choi Differential expression of circulating microRNAs in lactating Holstein and Jersey cows exposed to heat stress Journal of Animal Reproduction and Biotechnology circulating microrna heat stress holstein jersey lactation |
| title | Differential expression of circulating microRNAs in lactating Holstein and Jersey cows exposed to heat stress |
| title_full | Differential expression of circulating microRNAs in lactating Holstein and Jersey cows exposed to heat stress |
| title_fullStr | Differential expression of circulating microRNAs in lactating Holstein and Jersey cows exposed to heat stress |
| title_full_unstemmed | Differential expression of circulating microRNAs in lactating Holstein and Jersey cows exposed to heat stress |
| title_short | Differential expression of circulating microRNAs in lactating Holstein and Jersey cows exposed to heat stress |
| title_sort | differential expression of circulating micrornas in lactating holstein and jersey cows exposed to heat stress |
| topic | circulating microrna heat stress holstein jersey lactation |
| url | https://www.e-jarb.org/journal/view.html?uid=2708&vmd=Full |
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