High-confidence assessment of functional impact of human mitochondrial non-synonymous genome variations by APOGEE.

24,189 are all the possible non-synonymous amino acid changes potentially affecting the human mitochondrial DNA. Only a tiny subset was functionally evaluated with certainty so far, while the pathogenicity of the vast majority was only assessed in-silico by software predictors. Since these tools pro...

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Main Authors: Stefano Castellana, Caterina Fusilli, Gianluigi Mazzoccoli, Tommaso Biagini, Daniele Capocefalo, Massimo Carella, Angelo Luigi Vescovi, Tommaso Mazza
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2017-06-01
Series:PLoS Computational Biology
Online Access:https://journals.plos.org/ploscompbiol/article/file?id=10.1371/journal.pcbi.1005628&type=printable
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author Stefano Castellana
Caterina Fusilli
Gianluigi Mazzoccoli
Tommaso Biagini
Daniele Capocefalo
Massimo Carella
Angelo Luigi Vescovi
Tommaso Mazza
author_facet Stefano Castellana
Caterina Fusilli
Gianluigi Mazzoccoli
Tommaso Biagini
Daniele Capocefalo
Massimo Carella
Angelo Luigi Vescovi
Tommaso Mazza
author_sort Stefano Castellana
collection DOAJ
description 24,189 are all the possible non-synonymous amino acid changes potentially affecting the human mitochondrial DNA. Only a tiny subset was functionally evaluated with certainty so far, while the pathogenicity of the vast majority was only assessed in-silico by software predictors. Since these tools proved to be rather incongruent, we have designed and implemented APOGEE, a machine-learning algorithm that outperforms all existing prediction methods in estimating the harmfulness of mitochondrial non-synonymous genome variations. We provide a detailed description of the underlying algorithm, of the selected and manually curated training and test sets of variants, as well as of its classification ability.
format Article
id doaj-art-1e27cf97c79e44cc84c7c1abe13134fd
institution OA Journals
issn 1553-734X
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language English
publishDate 2017-06-01
publisher Public Library of Science (PLoS)
record_format Article
series PLoS Computational Biology
spelling doaj-art-1e27cf97c79e44cc84c7c1abe13134fd2025-08-20T02:03:47ZengPublic Library of Science (PLoS)PLoS Computational Biology1553-734X1553-73582017-06-01136e100562810.1371/journal.pcbi.1005628High-confidence assessment of functional impact of human mitochondrial non-synonymous genome variations by APOGEE.Stefano CastellanaCaterina FusilliGianluigi MazzoccoliTommaso BiaginiDaniele CapocefaloMassimo CarellaAngelo Luigi VescoviTommaso Mazza24,189 are all the possible non-synonymous amino acid changes potentially affecting the human mitochondrial DNA. Only a tiny subset was functionally evaluated with certainty so far, while the pathogenicity of the vast majority was only assessed in-silico by software predictors. Since these tools proved to be rather incongruent, we have designed and implemented APOGEE, a machine-learning algorithm that outperforms all existing prediction methods in estimating the harmfulness of mitochondrial non-synonymous genome variations. We provide a detailed description of the underlying algorithm, of the selected and manually curated training and test sets of variants, as well as of its classification ability.https://journals.plos.org/ploscompbiol/article/file?id=10.1371/journal.pcbi.1005628&type=printable
spellingShingle Stefano Castellana
Caterina Fusilli
Gianluigi Mazzoccoli
Tommaso Biagini
Daniele Capocefalo
Massimo Carella
Angelo Luigi Vescovi
Tommaso Mazza
High-confidence assessment of functional impact of human mitochondrial non-synonymous genome variations by APOGEE.
PLoS Computational Biology
title High-confidence assessment of functional impact of human mitochondrial non-synonymous genome variations by APOGEE.
title_full High-confidence assessment of functional impact of human mitochondrial non-synonymous genome variations by APOGEE.
title_fullStr High-confidence assessment of functional impact of human mitochondrial non-synonymous genome variations by APOGEE.
title_full_unstemmed High-confidence assessment of functional impact of human mitochondrial non-synonymous genome variations by APOGEE.
title_short High-confidence assessment of functional impact of human mitochondrial non-synonymous genome variations by APOGEE.
title_sort high confidence assessment of functional impact of human mitochondrial non synonymous genome variations by apogee
url https://journals.plos.org/ploscompbiol/article/file?id=10.1371/journal.pcbi.1005628&type=printable
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