THE DEVELOPMENT OF CONDUCTOMETRIC BIOSENSOR FOR DETERMINATION OF URIC ACID CONCENTRATION IN HUMAN SERUM USING SCREEN PRINTED CARBON ELECTRODE (SPCE) – NATA DE COCO
The control of uric acid is important. A high level of uric acid can cause gout disease. Therefore a simple, fast, and accurate method for uric acid determination is required. In this research a conductometric biosensor has been developed by SPCE – Nata de coco for uric acid determination. The prepa...
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Universitas Sebelas Maret
2015-09-01
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| Series: | Alchemy |
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| Online Access: | https://jurnal.uns.ac.id/index.php?journal=alchemy&page=article&op=view&path[]=724 |
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| author | Abdi Naufal Ramadhan Luluil Maknun Noerma Juli Azhari |
| author_facet | Abdi Naufal Ramadhan Luluil Maknun Noerma Juli Azhari |
| author_sort | Abdi Naufal Ramadhan |
| collection | DOAJ |
| description | The control of uric acid is important. A high level of uric acid can cause gout disease. Therefore a simple, fast, and accurate method for uric acid determination is required. In this research a conductometric biosensor has been developed by SPCE – Nata de coco for uric acid determination. The prepared biosensor was optimized to get a good performance of biosensor and it is applicable for human serum samples. The optimized variables were enzyme concentration, membrane thickness and pH solution. The various enzyme concentration were 6 μg/mL; 12 μg/mL; 18μg/mL; 24 μg/mL. The various membrane thickness were 5 μm; 10 μm; 15 μm. Meanwhile, the various pH solution were 7; 7.5; 8; 8.5; 9. The optimum enzime concentration was 18 µg/mL with the membrane thickness and pH were 5 µm and 8, respectively. The prepared biosensor can determine the uric acid concentration at range of 0 – 1.2 ppm with the sensitivity of 7.74 µS/ppm and the limit detection is 0 ppm. The biosensor was applied to uric acid detection in human serum with accuracy of 95 %. |
| format | Article |
| id | doaj-art-1e212fbf95d249df947833ef93becedb |
| institution | DOAJ |
| issn | 1412-4092 2443-4183 |
| language | English |
| publishDate | 2015-09-01 |
| publisher | Universitas Sebelas Maret |
| record_format | Article |
| series | Alchemy |
| spelling | doaj-art-1e212fbf95d249df947833ef93becedb2025-08-20T02:57:57ZengUniversitas Sebelas MaretAlchemy1412-40922443-41832015-09-0111219219910.20961/alchemy.v11i2.724THE DEVELOPMENT OF CONDUCTOMETRIC BIOSENSOR FOR DETERMINATION OF URIC ACID CONCENTRATION IN HUMAN SERUM USING SCREEN PRINTED CARBON ELECTRODE (SPCE) – NATA DE COCOAbdi Naufal Ramadhan0Luluil Maknun1Noerma Juli Azhari2Jurusan Kimia, FMIPA, Universitas Brawijaya, Jl. Veteran, Malang 65145 telp. (0341) 575838Jurusan Kimia, FMIPA, Universitas Brawijaya, Jl. Veteran, Malang 65145 telp. (0341) 575838Jurusan Kimia, FMIPA, Universitas Brawijaya, Jl. Veteran, Malang 65145 telp. (0341) 575838The control of uric acid is important. A high level of uric acid can cause gout disease. Therefore a simple, fast, and accurate method for uric acid determination is required. In this research a conductometric biosensor has been developed by SPCE – Nata de coco for uric acid determination. The prepared biosensor was optimized to get a good performance of biosensor and it is applicable for human serum samples. The optimized variables were enzyme concentration, membrane thickness and pH solution. The various enzyme concentration were 6 μg/mL; 12 μg/mL; 18μg/mL; 24 μg/mL. The various membrane thickness were 5 μm; 10 μm; 15 μm. Meanwhile, the various pH solution were 7; 7.5; 8; 8.5; 9. The optimum enzime concentration was 18 µg/mL with the membrane thickness and pH were 5 µm and 8, respectively. The prepared biosensor can determine the uric acid concentration at range of 0 – 1.2 ppm with the sensitivity of 7.74 µS/ppm and the limit detection is 0 ppm. The biosensor was applied to uric acid detection in human serum with accuracy of 95 %.https://jurnal.uns.ac.id/index.php?journal=alchemy&page=article&op=view&path[]=724conductometricSPCE-nata de cocouric acid biosensor |
| spellingShingle | Abdi Naufal Ramadhan Luluil Maknun Noerma Juli Azhari THE DEVELOPMENT OF CONDUCTOMETRIC BIOSENSOR FOR DETERMINATION OF URIC ACID CONCENTRATION IN HUMAN SERUM USING SCREEN PRINTED CARBON ELECTRODE (SPCE) – NATA DE COCO Alchemy conductometric SPCE-nata de coco uric acid biosensor |
| title | THE DEVELOPMENT OF CONDUCTOMETRIC BIOSENSOR FOR DETERMINATION OF URIC ACID CONCENTRATION IN HUMAN SERUM USING SCREEN PRINTED CARBON ELECTRODE (SPCE) – NATA DE COCO |
| title_full | THE DEVELOPMENT OF CONDUCTOMETRIC BIOSENSOR FOR DETERMINATION OF URIC ACID CONCENTRATION IN HUMAN SERUM USING SCREEN PRINTED CARBON ELECTRODE (SPCE) – NATA DE COCO |
| title_fullStr | THE DEVELOPMENT OF CONDUCTOMETRIC BIOSENSOR FOR DETERMINATION OF URIC ACID CONCENTRATION IN HUMAN SERUM USING SCREEN PRINTED CARBON ELECTRODE (SPCE) – NATA DE COCO |
| title_full_unstemmed | THE DEVELOPMENT OF CONDUCTOMETRIC BIOSENSOR FOR DETERMINATION OF URIC ACID CONCENTRATION IN HUMAN SERUM USING SCREEN PRINTED CARBON ELECTRODE (SPCE) – NATA DE COCO |
| title_short | THE DEVELOPMENT OF CONDUCTOMETRIC BIOSENSOR FOR DETERMINATION OF URIC ACID CONCENTRATION IN HUMAN SERUM USING SCREEN PRINTED CARBON ELECTRODE (SPCE) – NATA DE COCO |
| title_sort | development of conductometric biosensor for determination of uric acid concentration in human serum using screen printed carbon electrode spce nata de coco |
| topic | conductometric SPCE-nata de coco uric acid biosensor |
| url | https://jurnal.uns.ac.id/index.php?journal=alchemy&page=article&op=view&path[]=724 |
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