Whole Genome Sequencing and Comparative Genomic Analysis of <i>Pseudomonas aeruginosa</i> SF416, a Potential Broad-Spectrum Biocontrol Agent Against <i>Xanthomonas oryzae</i> pv. <i>oryzae</i>
Rice is one of the most important staple crops worldwide. However, the bacterial blight of rice caused by <i>Xanthomonas oryzae</i> pv. <i>oryzae</i> (<i>Xoo</i>) poses a major threat to the production of rice. In this study, we isolated and identified the strain...
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| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-11-01
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| Series: | Microorganisms |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2607/12/11/2263 |
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| Summary: | Rice is one of the most important staple crops worldwide. However, the bacterial blight of rice caused by <i>Xanthomonas oryzae</i> pv. <i>oryzae</i> (<i>Xoo</i>) poses a major threat to the production of rice. In this study, we isolated and identified the strain <i>Pseudomonas aeruginosa</i> SF416, which exhibited significant antagonistic activity against <i>Xoo</i>, from a soil sample collected in a winter wheat field in Shannanzhalang County, Tibet, China. The bacterial solution (BS) and cell-free supernatant (CFS) of SF416 had significant prevention effects for the bacterial blight of rice, with an efficacy of 45.1% and 34.18%, respectively, while they exhibited a slightly lower therapeutic efficiency of 31.64% and 25.09%. The genomic analysis showed that <i>P. aeruginosa</i> SF416 contains genes involved in cell motility, colonization, cold and hot shock proteins, antibiotic resistance, and plant growth promotion. SF416 also harbors two sets of phenazine-1-carboxylic acid (PCA) synthesis gene clusters, phz1 (<i>phzA1</i>-<i>G1</i>) and phz2 (<i>phzA2</i>-<i>G2</i>), and other phenozine product-synthesis--related genes <i>phzS</i>, <i>phzM</i>, and <i>phzH</i>, as well as genes in the SF416 genome that share high similarity with the ones in the genomes of <i>P</i>. <i>aeruginosa</i> M18, suggesting that the two sets of PCA synthesis gene clusters are responsible for the antagonistic effect of SF416 against <i>Xoo</i>. A comparative antiSMASH analysis revealed that <i>P</i>. <i>aeruginosa</i> SF416 contains 17 gene clusters related to secondary metabolite synthesis, 7 of which, encoding for pyochelin, azetidomonamide A/B, L-2-amino-4-methoxy-trans-3-butenoic acid, hydrogen cyanide, pyocyanine, pseudopaline, and bicyclomycin, are conserved in strains of <i>P</i>. <i>aeruginosa.</i> Moreover, SF416 can produce protease and siderophores and display a broad-spectrum antagonistic activity against various major plant pathogenic bacteria and fungi. The results suggest that <i>P</i>. <i>aeruginosa</i> SF416 could be a potential candidate agent for the bacterial blight of rice. |
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| ISSN: | 2076-2607 |