The Development of a Human Respiratory Mucosa-on-a-Chip Using Human Turbinate-Derived Mesenchymal Stem Cells
<i>Background and Objectives</i>: This study aimed to investigate the influence of a respiratory mucosa-on-a-chip on the respiratory epithelial differentiation potential of human nasal inferior turbinate-derived stem cells (hNTSCs). <i>Materials and Methods</i>: After isolati...
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2024-10-01
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| author | Do Hyun Kim Sang Hi Park Mi-Yeon Kwon Chae-Yoon Lim Sun Hwa Park David W. Jang Se Hwan Hwang Sung Won Kim |
| author_facet | Do Hyun Kim Sang Hi Park Mi-Yeon Kwon Chae-Yoon Lim Sun Hwa Park David W. Jang Se Hwan Hwang Sung Won Kim |
| author_sort | Do Hyun Kim |
| collection | DOAJ |
| description | <i>Background and Objectives</i>: This study aimed to investigate the influence of a respiratory mucosa-on-a-chip on the respiratory epithelial differentiation potential of human nasal inferior turbinate-derived stem cells (hNTSCs). <i>Materials and Methods</i>: After isolating hNTSCs from five patients, we divided the samples from the patients into the study group with a mucosa-on-a-chip and the control group with conventional differentiation (using conventional differentiation methods). The respiratory epithelial differentiation potential of hNTSCs was analyzed by histology and gene expression. <i>Results</i>: In the quantitative analysis, PCR showed that the hNTSCs expressed the cytokeratin genes (KRT13, 14), transformation-related protein P63 (TP63), and vimentin of basal cells in the airway epithelium at higher levels, but cytokeratin genes (KRT6) at lower levels, in the mucosa-on-a-chip than in conventional differentiation. In the cytokine analysis (GM-CSF, IFNr, IL-1a, IL-1b, IL-4, IL-5, IL-10, IL-12(p70), IL-13, IL-17A, IL-17E/IL-25, RANTES, TNFa, IL-6, and IL-8), the expressions of IFNr, IL-13, RANTES, TNFa, IL-6, and IL-8 were significantly upregulated in the mucosa-on-a-chip than in conventional differentiation. <i>Conclusions</i>: We conclude that the human respiratory mucosa-on-a-chip using human turbinate-derived mesenchymal stem cells allows the respiratory differentiation of hNTSCs and shows the difference in gene and cytokine expression, which could serve as an alternative to conventional differentiation for the production of functionally competent hNTSCs for future clinical applications. |
| format | Article |
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| institution | DOAJ |
| issn | 1010-660X 1648-9144 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | MDPI AG |
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| series | Medicina |
| spelling | doaj-art-1e13694683fe4e2e9db1ce7adf83f7932025-08-20T02:48:06ZengMDPI AGMedicina1010-660X1648-91442024-10-016011174110.3390/medicina60111741The Development of a Human Respiratory Mucosa-on-a-Chip Using Human Turbinate-Derived Mesenchymal Stem CellsDo Hyun Kim0Sang Hi Park1Mi-Yeon Kwon2Chae-Yoon Lim3Sun Hwa Park4David W. Jang5Se Hwan Hwang6Sung Won Kim7Department of Otolaryngology-Head and Neck Surgery, College of Medicine, The Catholic University of Korea, Seoul 06591, Republic of KoreaInstitute of Clinical Medicine Research, College of Medicine, Catholic University of Korea, Seoul 06591, Republic of KoreaInstitute of Clinical Medicine Research, College of Medicine, Catholic University of Korea, Seoul 06591, Republic of KoreaInstitute of Clinical Medicine Research, College of Medicine, Catholic University of Korea, Seoul 06591, Republic of KoreaPostech-Catholic Biomedical Engineering Institute, College of Medicine, The Catholic University of Korea, Seoul 06591, Republic of KoreaDepartment of Head and Neck Surgery & Communication Sciences, Duke University School of Medicine, Durham, NC 27710, USADepartment of Otolaryngology-Head and Neck Surgery, College of Medicine, The Catholic University of Korea, Seoul 06591, Republic of KoreaDepartment of Otolaryngology-Head and Neck Surgery, College of Medicine, The Catholic University of Korea, Seoul 06591, Republic of Korea<i>Background and Objectives</i>: This study aimed to investigate the influence of a respiratory mucosa-on-a-chip on the respiratory epithelial differentiation potential of human nasal inferior turbinate-derived stem cells (hNTSCs). <i>Materials and Methods</i>: After isolating hNTSCs from five patients, we divided the samples from the patients into the study group with a mucosa-on-a-chip and the control group with conventional differentiation (using conventional differentiation methods). The respiratory epithelial differentiation potential of hNTSCs was analyzed by histology and gene expression. <i>Results</i>: In the quantitative analysis, PCR showed that the hNTSCs expressed the cytokeratin genes (KRT13, 14), transformation-related protein P63 (TP63), and vimentin of basal cells in the airway epithelium at higher levels, but cytokeratin genes (KRT6) at lower levels, in the mucosa-on-a-chip than in conventional differentiation. In the cytokine analysis (GM-CSF, IFNr, IL-1a, IL-1b, IL-4, IL-5, IL-10, IL-12(p70), IL-13, IL-17A, IL-17E/IL-25, RANTES, TNFa, IL-6, and IL-8), the expressions of IFNr, IL-13, RANTES, TNFa, IL-6, and IL-8 were significantly upregulated in the mucosa-on-a-chip than in conventional differentiation. <i>Conclusions</i>: We conclude that the human respiratory mucosa-on-a-chip using human turbinate-derived mesenchymal stem cells allows the respiratory differentiation of hNTSCs and shows the difference in gene and cytokine expression, which could serve as an alternative to conventional differentiation for the production of functionally competent hNTSCs for future clinical applications.https://www.mdpi.com/1648-9144/60/11/1741microfluidicsrespiratory systemstem cellsculturehumans |
| spellingShingle | Do Hyun Kim Sang Hi Park Mi-Yeon Kwon Chae-Yoon Lim Sun Hwa Park David W. Jang Se Hwan Hwang Sung Won Kim The Development of a Human Respiratory Mucosa-on-a-Chip Using Human Turbinate-Derived Mesenchymal Stem Cells Medicina microfluidics respiratory system stem cells culture humans |
| title | The Development of a Human Respiratory Mucosa-on-a-Chip Using Human Turbinate-Derived Mesenchymal Stem Cells |
| title_full | The Development of a Human Respiratory Mucosa-on-a-Chip Using Human Turbinate-Derived Mesenchymal Stem Cells |
| title_fullStr | The Development of a Human Respiratory Mucosa-on-a-Chip Using Human Turbinate-Derived Mesenchymal Stem Cells |
| title_full_unstemmed | The Development of a Human Respiratory Mucosa-on-a-Chip Using Human Turbinate-Derived Mesenchymal Stem Cells |
| title_short | The Development of a Human Respiratory Mucosa-on-a-Chip Using Human Turbinate-Derived Mesenchymal Stem Cells |
| title_sort | development of a human respiratory mucosa on a chip using human turbinate derived mesenchymal stem cells |
| topic | microfluidics respiratory system stem cells culture humans |
| url | https://www.mdpi.com/1648-9144/60/11/1741 |
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