Correlative light microscopy for high-content screening
High-throughput microscopy is an effective tool for rapidly collecting data on a large scale. However, high throughput comes at the cost of low spatial resolution. Here we introduce correlative light microscopy by combining fast automated widefield imaging, confocal microscopy and super-resolution m...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2013-11-01
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| Series: | BioTechniques |
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| Online Access: | https://www.future-science.com/doi/10.2144/000114099 |
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| _version_ | 1850153032951529472 |
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| author | Benjamin Flottmann Manuel Gunkel Tautvydas Lisauskas Mike Heilemann Vytaute Starkuviene Jürgen Reymann Holger Erfle |
| author_facet | Benjamin Flottmann Manuel Gunkel Tautvydas Lisauskas Mike Heilemann Vytaute Starkuviene Jürgen Reymann Holger Erfle |
| author_sort | Benjamin Flottmann |
| collection | DOAJ |
| description | High-throughput microscopy is an effective tool for rapidly collecting data on a large scale. However, high throughput comes at the cost of low spatial resolution. Here we introduce correlative light microscopy by combining fast automated widefield imaging, confocal microscopy and super-resolution microscopy. We demonstrate the potential of this approach for scalable experiments. The workflow consists of a robust approach for selecting cells of interest on a wide-field screening microscope at low resolution and subsequently re-localizing those cells with micrometer precision for confocal and super-resolution imaging. As a case study, we visualized and quantified cis- and trans-Golgi markers at increasing resolution. |
| format | Article |
| id | doaj-art-1e0deac1f9c94de59b8dce558b31850c |
| institution | OA Journals |
| issn | 0736-6205 1940-9818 |
| language | English |
| publishDate | 2013-11-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | BioTechniques |
| spelling | doaj-art-1e0deac1f9c94de59b8dce558b31850c2025-08-20T02:25:50ZengTaylor & Francis GroupBioTechniques0736-62051940-98182013-11-0155524325210.2144/000114099Correlative light microscopy for high-content screeningBenjamin Flottmann0Manuel Gunkel1Tautvydas Lisauskas2Mike Heilemann3Vytaute Starkuviene4Jürgen Reymann5Holger Erfle61BioQuant Centre, Heidelberg University, Heidelberg, Germany1BioQuant Centre, Heidelberg University, Heidelberg, Germany1BioQuant Centre, Heidelberg University, Heidelberg, Germany1BioQuant Centre, Heidelberg University, Heidelberg, Germany1BioQuant Centre, Heidelberg University, Heidelberg, Germany1BioQuant Centre, Heidelberg University, Heidelberg, Germany1BioQuant Centre, Heidelberg University, Heidelberg, GermanyHigh-throughput microscopy is an effective tool for rapidly collecting data on a large scale. However, high throughput comes at the cost of low spatial resolution. Here we introduce correlative light microscopy by combining fast automated widefield imaging, confocal microscopy and super-resolution microscopy. We demonstrate the potential of this approach for scalable experiments. The workflow consists of a robust approach for selecting cells of interest on a wide-field screening microscope at low resolution and subsequently re-localizing those cells with micrometer precision for confocal and super-resolution imaging. As a case study, we visualized and quantified cis- and trans-Golgi markers at increasing resolution.https://www.future-science.com/doi/10.2144/000114099super-resolution microscopydSTORMhigh-content screeningcorrelative microscopyGolgi complex |
| spellingShingle | Benjamin Flottmann Manuel Gunkel Tautvydas Lisauskas Mike Heilemann Vytaute Starkuviene Jürgen Reymann Holger Erfle Correlative light microscopy for high-content screening BioTechniques super-resolution microscopy dSTORM high-content screening correlative microscopy Golgi complex |
| title | Correlative light microscopy for high-content screening |
| title_full | Correlative light microscopy for high-content screening |
| title_fullStr | Correlative light microscopy for high-content screening |
| title_full_unstemmed | Correlative light microscopy for high-content screening |
| title_short | Correlative light microscopy for high-content screening |
| title_sort | correlative light microscopy for high content screening |
| topic | super-resolution microscopy dSTORM high-content screening correlative microscopy Golgi complex |
| url | https://www.future-science.com/doi/10.2144/000114099 |
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