Nonadiene isomers as potential green flavor components of autoxidizing fish oil

Abstract Fish oil oxidation leads to the formation of volatile compounds that contribute to undesirable off-flavors, primarily through free radical mechanisms that produce hydroperoxides and their decomposition products, including aldehydes, ketones, and furan derivatives. This study focuses on the...

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Main Authors: Habibollah Faraji, Robert C. Lindsay, Shun Wada, Salam A. Ibrahim, Majid Aminzare, Reza Tahergorabi
Format: Article
Language:English
Published: Springer 2025-05-01
Series:Discover Food
Subjects:
Online Access:https://doi.org/10.1007/s44187-025-00440-4
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author Habibollah Faraji
Robert C. Lindsay
Shun Wada
Salam A. Ibrahim
Majid Aminzare
Reza Tahergorabi
author_facet Habibollah Faraji
Robert C. Lindsay
Shun Wada
Salam A. Ibrahim
Majid Aminzare
Reza Tahergorabi
author_sort Habibollah Faraji
collection DOAJ
description Abstract Fish oil oxidation leads to the formation of volatile compounds that contribute to undesirable off-flavors, primarily through free radical mechanisms that produce hydroperoxides and their decomposition products, including aldehydes, ketones, and furan derivatives. This study focuses on the identification of volatile compounds formed during the autoxidation of menhaden oil under three different conditions: (1) exposure to light for 11 days, (2) heating at 121 °C for 80 min, and (3) storage in the dark at 40 °C for 48 h. Gas chromatography-mass spectrometry (GC–MS) analysis confirmed the production of three isomers of nonadiene, including the potent aroma compound 2,4-nonadiene, which has a low aroma threshold (7.8 ppb in water) and contributes to green, fishy off-notes. Notably, 2,4-nonadiene is predominantly derived from omega-6 fatty acids rather than omega-3, a novel finding. Additionally, olfactory detection identified green compounds between C4 and C5 ethyl esters from oxidized menhaden oil, though GC–MS spectra suggested co-elution of compounds, complicating identification. This research highlights the unique formation pathways of nonadiene isomers and offers new insights into controlling oxidative off-flavors in fish oil products, with implications for improving product quality and shelf life.
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spelling doaj-art-1e0bf7f39f6a43d1bd4cfbc22fdf2a2f2025-08-20T03:08:25ZengSpringerDiscover Food2731-42862025-05-015111410.1007/s44187-025-00440-4Nonadiene isomers as potential green flavor components of autoxidizing fish oilHabibollah Faraji0Robert C. Lindsay1Shun Wada2Salam A. Ibrahim3Majid Aminzare4Reza Tahergorabi5Food and Nutritional Sciences Program, North Carolina Agricultural &Technical State UniversityDepartment of Food Science, University of Wisconsin-MadisonDepartment of Food Science and Technology, Tokyo University of Marine Science and TechnologyFood and Nutritional Sciences Program, North Carolina Agricultural &Technical State UniversityDepartment of Food Safety and Hygiene, School of Public Health, Zanjan University of Medical SciencesFood and Nutritional Sciences Program, North Carolina Agricultural &Technical State UniversityAbstract Fish oil oxidation leads to the formation of volatile compounds that contribute to undesirable off-flavors, primarily through free radical mechanisms that produce hydroperoxides and their decomposition products, including aldehydes, ketones, and furan derivatives. This study focuses on the identification of volatile compounds formed during the autoxidation of menhaden oil under three different conditions: (1) exposure to light for 11 days, (2) heating at 121 °C for 80 min, and (3) storage in the dark at 40 °C for 48 h. Gas chromatography-mass spectrometry (GC–MS) analysis confirmed the production of three isomers of nonadiene, including the potent aroma compound 2,4-nonadiene, which has a low aroma threshold (7.8 ppb in water) and contributes to green, fishy off-notes. Notably, 2,4-nonadiene is predominantly derived from omega-6 fatty acids rather than omega-3, a novel finding. Additionally, olfactory detection identified green compounds between C4 and C5 ethyl esters from oxidized menhaden oil, though GC–MS spectra suggested co-elution of compounds, complicating identification. This research highlights the unique formation pathways of nonadiene isomers and offers new insights into controlling oxidative off-flavors in fish oil products, with implications for improving product quality and shelf life.https://doi.org/10.1007/s44187-025-00440-4Nonadiene isomersFish oilOxidationGreen flavor
spellingShingle Habibollah Faraji
Robert C. Lindsay
Shun Wada
Salam A. Ibrahim
Majid Aminzare
Reza Tahergorabi
Nonadiene isomers as potential green flavor components of autoxidizing fish oil
Discover Food
Nonadiene isomers
Fish oil
Oxidation
Green flavor
title Nonadiene isomers as potential green flavor components of autoxidizing fish oil
title_full Nonadiene isomers as potential green flavor components of autoxidizing fish oil
title_fullStr Nonadiene isomers as potential green flavor components of autoxidizing fish oil
title_full_unstemmed Nonadiene isomers as potential green flavor components of autoxidizing fish oil
title_short Nonadiene isomers as potential green flavor components of autoxidizing fish oil
title_sort nonadiene isomers as potential green flavor components of autoxidizing fish oil
topic Nonadiene isomers
Fish oil
Oxidation
Green flavor
url https://doi.org/10.1007/s44187-025-00440-4
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