Comparative evaluation of three polymerase chain reaction primer sets for accurate molecular detection of Trypanosoma lewisi in wild rodents in Indonesia

Comparative evaluation of three polymerase chain reaction primer sets for accurate molecular detection of Trypanosoma lewisi in wild rodents in Indonesia

Background and Aim: Trypanosoma lewisi is a flea-transmitted protozoan parasite commonly infecting rodents and posing zoonotic risks. Conventional diagnostics such as blood smear and serology often fail in low parasitemia conditions. Molecular diagnostics using polymerase chain reaction (PCR) offer...

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Bibliographic Details
Main Authors: Aditya Yudhana, Gusti Ayu Illiyin Putri Santosa, April Hari Wardhana, Frenky Laksana Putra, Ryanka Edila, Dyah Haryuningtyas Sawitri, Ratih Novita Praja, Muhammad Aqil Kurnianto, Aldi Gusnizar Rizaldy Tanjung, Marc Desquesnes, Makoto Matsubayashi
Format: Article
Language:English
Published: Veterinary World 2025-08-01
Series:Veterinary World
Subjects:
diagnostic validation
flea-transmitted protozoa
molecular diagnostics
neglected disease
polymerase chain reaction primers
public health
rodent-borne zoonosis
southeast asia
trypanosoma lewisi
Online Access:https://www.veterinaryworld.org/Vol.18/August-2025/21.pdf
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