Molecular analysis of the adaptive response in Salmonella Typhimurium after starvation in salty conditions
Introduction: The pathogenic bacterium Salmonella enterica serovar Typhimurium elicits a variety of genetic programs to adapt to stress conditions encountered within hostile environments such as host phagocytes and preserved food. Methodology: In this work, differential display (DD) methodology was...
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The Journal of Infection in Developing Countries
2016-01-01
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| Series: | Journal of Infection in Developing Countries |
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| Online Access: | https://jidc.org/index.php/journal/article/view/7033 |
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| author | Hamouda Elabed Abderrahmen Merghni Rim Hamza Amina Bakhrouf Kamel Gaddour |
| author_facet | Hamouda Elabed Abderrahmen Merghni Rim Hamza Amina Bakhrouf Kamel Gaddour |
| author_sort | Hamouda Elabed |
| collection | DOAJ |
| description | Introduction: The pathogenic bacterium Salmonella enterica serovar Typhimurium elicits a variety of genetic programs to adapt to stress conditions encountered within hostile environments such as host phagocytes and preserved food.
Methodology: In this work, differential display (DD) methodology was used to investigate the effect of one month starvation in a salty microcosm (0.5 M NaCl) on transcript profiling in a Salmonella Typhimurium LT2 strain. cDNA fragments resulting from differentially expressed mRNA were eluted from the gel, re-amplified, cloned, and then sequenced.
Results: A total of 21 differentially expressed bands were detected by DD reverse transcription-polymerase chain reaction (RT-PCR). However, only 12 of them were successfully identified as upregulated genes in stressed cells. Based on the sequencing data and BLAST analysis, these genes were sopA, ssaD, yhhK, gmK, cspC, uspA, ompR, phoP, stcC, fimA, acrA,and yehZ. As a confirmation of the differential expression, RT-PCR was carried out using a set of specific primers. Remarkably, the expression levels of these genes were significantly increased in starved bacteria compared to standard laboratory conditions.
Conclusions: Our results indicate that the starvation of Salmonella Typhimurium over one month in a salty microcosm changes the expression of stress proteins, response regulator in a two-component system, outer membrane proteins, effector proteins translocated by Salmonella pathogenicity island SPI1 and SPI2 type III secretion systems (TTSS), several metabolic enzymes, efflux pumps, and transport proteins. This suggests that the expression of the identified genes is important for the response of this pathogen to starvation in salt.
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| format | Article |
| id | doaj-art-1de89652da274ffbaa013e3b7977d29e |
| institution | OA Journals |
| issn | 1972-2680 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | The Journal of Infection in Developing Countries |
| record_format | Article |
| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-1de89652da274ffbaa013e3b7977d29e2025-08-20T02:16:18ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802016-01-01100110.3855/jidc.7033Molecular analysis of the adaptive response in Salmonella Typhimurium after starvation in salty conditionsHamouda Elabed0Abderrahmen Merghni1Rim Hamza2Amina Bakhrouf3Kamel Gaddour4Faculty of Pharmacy, University of Monastir, Monastir, TunisiaFaculty of Pharmacy, University of Monastir, Monastir, TunisiaISBM, University of Monastir, Monastir, TunisiaFaculty of Pharmacy, University of Monastir, Monastir, TunisiaISBM, University of Monastir, Monastir, TunisiaIntroduction: The pathogenic bacterium Salmonella enterica serovar Typhimurium elicits a variety of genetic programs to adapt to stress conditions encountered within hostile environments such as host phagocytes and preserved food. Methodology: In this work, differential display (DD) methodology was used to investigate the effect of one month starvation in a salty microcosm (0.5 M NaCl) on transcript profiling in a Salmonella Typhimurium LT2 strain. cDNA fragments resulting from differentially expressed mRNA were eluted from the gel, re-amplified, cloned, and then sequenced. Results: A total of 21 differentially expressed bands were detected by DD reverse transcription-polymerase chain reaction (RT-PCR). However, only 12 of them were successfully identified as upregulated genes in stressed cells. Based on the sequencing data and BLAST analysis, these genes were sopA, ssaD, yhhK, gmK, cspC, uspA, ompR, phoP, stcC, fimA, acrA,and yehZ. As a confirmation of the differential expression, RT-PCR was carried out using a set of specific primers. Remarkably, the expression levels of these genes were significantly increased in starved bacteria compared to standard laboratory conditions. Conclusions: Our results indicate that the starvation of Salmonella Typhimurium over one month in a salty microcosm changes the expression of stress proteins, response regulator in a two-component system, outer membrane proteins, effector proteins translocated by Salmonella pathogenicity island SPI1 and SPI2 type III secretion systems (TTSS), several metabolic enzymes, efflux pumps, and transport proteins. This suggests that the expression of the identified genes is important for the response of this pathogen to starvation in salt. https://jidc.org/index.php/journal/article/view/7033Salmonellastarvationsalt stressgene expressionDDRT-PCR |
| spellingShingle | Hamouda Elabed Abderrahmen Merghni Rim Hamza Amina Bakhrouf Kamel Gaddour Molecular analysis of the adaptive response in Salmonella Typhimurium after starvation in salty conditions Journal of Infection in Developing Countries Salmonella starvation salt stress gene expression DDRT-PCR |
| title | Molecular analysis of the adaptive response in Salmonella Typhimurium after starvation in salty conditions |
| title_full | Molecular analysis of the adaptive response in Salmonella Typhimurium after starvation in salty conditions |
| title_fullStr | Molecular analysis of the adaptive response in Salmonella Typhimurium after starvation in salty conditions |
| title_full_unstemmed | Molecular analysis of the adaptive response in Salmonella Typhimurium after starvation in salty conditions |
| title_short | Molecular analysis of the adaptive response in Salmonella Typhimurium after starvation in salty conditions |
| title_sort | molecular analysis of the adaptive response in salmonella typhimurium after starvation in salty conditions |
| topic | Salmonella starvation salt stress gene expression DDRT-PCR |
| url | https://jidc.org/index.php/journal/article/view/7033 |
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