Apolipoprotein M (ApoM) involved in the Nile tilapia (Oreochromis niloticus) against Streptococcus agalactiae infection

Apolipoprotein M (ApoM) is a type of apolipoprotein in the bloodstream, with a primary affinity for high-density lipoprotein (HDL). It exhibits tissue-specific expression and performs a variety of biological functions, including lipid transport and metabolism, as well as antibacterial and anti-infla...

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Main Authors: Xuyan Tan, Yongxiong Huang, Zhiqiang Zhang, Yuhang Dong, Kwaku Amoah, Yu Huang, Jichang Jian
Format: Article
Language:English
Published: Elsevier 2024-12-01
Series:Aquaculture Reports
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Online Access:http://www.sciencedirect.com/science/article/pii/S2352513424005945
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author Xuyan Tan
Yongxiong Huang
Zhiqiang Zhang
Yuhang Dong
Kwaku Amoah
Yu Huang
Jichang Jian
author_facet Xuyan Tan
Yongxiong Huang
Zhiqiang Zhang
Yuhang Dong
Kwaku Amoah
Yu Huang
Jichang Jian
author_sort Xuyan Tan
collection DOAJ
description Apolipoprotein M (ApoM) is a type of apolipoprotein in the bloodstream, with a primary affinity for high-density lipoprotein (HDL). It exhibits tissue-specific expression and performs a variety of biological functions, including lipid transport and metabolism, as well as antibacterial and anti-inflammatory effects. In order to elucidate the immune mechanism of ApoM in fish, we initiated the cloning of the ApoM gene in the widely studied Nile tilapia (Oreochromis niloticus) (On-ApoM) and examined its role during acute bacterial infection. The open reading frame (ORF) for On-ApoM encompasses 591 bp, encoding a protein of 196 amino acids. Notably, On-ApoM is mainly expressed in the liver of tilapia. After Streptococcus agalactiae stimulation, On-ApoM was activated to varying degrees in the liver, brain, spleen, head kidney, intestine, and other immune tissues of tilapia. While our study revealed that On-ApoM decreased bacterial load in the liver, On-ApoM again demonstrated the ability to inhibit inflammatory processes, consequently enhancing tilapia survival during acute bacterial infection. Alternatively, On-ApoM may play a role in modulating various immune pathways. These findings lay the groundwork for further exploration of the role of On-ApoM in fish immunity.
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publishDate 2024-12-01
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series Aquaculture Reports
spelling doaj-art-1dddf76cace44e649b29b0ba2a780d112025-08-20T02:51:34ZengElsevierAquaculture Reports2352-51342024-12-013910250610.1016/j.aqrep.2024.102506Apolipoprotein M (ApoM) involved in the Nile tilapia (Oreochromis niloticus) against Streptococcus agalactiae infectionXuyan Tan0Yongxiong Huang1Zhiqiang Zhang2Yuhang Dong3Kwaku Amoah4Yu Huang5Jichang Jian6Fisheries College of Guangdong Ocean University, Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture & Key Laboratory of Control for Disease of Aquatic Animals of Guangdong Higher Education Institutes, Zhanjiang 524088, ChinaFisheries College of Guangdong Ocean University, Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture & Key Laboratory of Control for Disease of Aquatic Animals of Guangdong Higher Education Institutes, Zhanjiang 524088, ChinaFisheries College of Guangdong Ocean University, Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture & Key Laboratory of Control for Disease of Aquatic Animals of Guangdong Higher Education Institutes, Zhanjiang 524088, ChinaFisheries College of Guangdong Ocean University, Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture & Key Laboratory of Control for Disease of Aquatic Animals of Guangdong Higher Education Institutes, Zhanjiang 524088, ChinaFisheries College of Guangdong Ocean University, Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture & Key Laboratory of Control for Disease of Aquatic Animals of Guangdong Higher Education Institutes, Zhanjiang 524088, ChinaFisheries College of Guangdong Ocean University, Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture & Key Laboratory of Control for Disease of Aquatic Animals of Guangdong Higher Education Institutes, Zhanjiang 524088, China; Guangdong Provincial Engineering Research Center for Aquatic Animal Health Assessment, Shenzhen, China; Corresponding authors at: Fisheries College of Guangdong Ocean University, Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture & Key Laboratory of Control for Disease of Aquatic Animals of Guangdong Higher Education Institutes, Zhanjiang 524088, China.Fisheries College of Guangdong Ocean University, Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture & Key Laboratory of Control for Disease of Aquatic Animals of Guangdong Higher Education Institutes, Zhanjiang 524088, China; Guangdong Provincial Engineering Research Center for Aquatic Animal Health Assessment, Shenzhen, China; Corresponding authors at: Fisheries College of Guangdong Ocean University, Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture & Key Laboratory of Control for Disease of Aquatic Animals of Guangdong Higher Education Institutes, Zhanjiang 524088, China.Apolipoprotein M (ApoM) is a type of apolipoprotein in the bloodstream, with a primary affinity for high-density lipoprotein (HDL). It exhibits tissue-specific expression and performs a variety of biological functions, including lipid transport and metabolism, as well as antibacterial and anti-inflammatory effects. In order to elucidate the immune mechanism of ApoM in fish, we initiated the cloning of the ApoM gene in the widely studied Nile tilapia (Oreochromis niloticus) (On-ApoM) and examined its role during acute bacterial infection. The open reading frame (ORF) for On-ApoM encompasses 591 bp, encoding a protein of 196 amino acids. Notably, On-ApoM is mainly expressed in the liver of tilapia. After Streptococcus agalactiae stimulation, On-ApoM was activated to varying degrees in the liver, brain, spleen, head kidney, intestine, and other immune tissues of tilapia. While our study revealed that On-ApoM decreased bacterial load in the liver, On-ApoM again demonstrated the ability to inhibit inflammatory processes, consequently enhancing tilapia survival during acute bacterial infection. Alternatively, On-ApoM may play a role in modulating various immune pathways. These findings lay the groundwork for further exploration of the role of On-ApoM in fish immunity.http://www.sciencedirect.com/science/article/pii/S2352513424005945Apolipoprotein MNile tilapiaInflammationStreptococcus agalactiaeImmune response
spellingShingle Xuyan Tan
Yongxiong Huang
Zhiqiang Zhang
Yuhang Dong
Kwaku Amoah
Yu Huang
Jichang Jian
Apolipoprotein M (ApoM) involved in the Nile tilapia (Oreochromis niloticus) against Streptococcus agalactiae infection
Aquaculture Reports
Apolipoprotein M
Nile tilapia
Inflammation
Streptococcus agalactiae
Immune response
title Apolipoprotein M (ApoM) involved in the Nile tilapia (Oreochromis niloticus) against Streptococcus agalactiae infection
title_full Apolipoprotein M (ApoM) involved in the Nile tilapia (Oreochromis niloticus) against Streptococcus agalactiae infection
title_fullStr Apolipoprotein M (ApoM) involved in the Nile tilapia (Oreochromis niloticus) against Streptococcus agalactiae infection
title_full_unstemmed Apolipoprotein M (ApoM) involved in the Nile tilapia (Oreochromis niloticus) against Streptococcus agalactiae infection
title_short Apolipoprotein M (ApoM) involved in the Nile tilapia (Oreochromis niloticus) against Streptococcus agalactiae infection
title_sort apolipoprotein m apom involved in the nile tilapia oreochromis niloticus against streptococcus agalactiae infection
topic Apolipoprotein M
Nile tilapia
Inflammation
Streptococcus agalactiae
Immune response
url http://www.sciencedirect.com/science/article/pii/S2352513424005945
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