Recognition of the sodium-dependent phosphate transporter NaPi2b by monoclonal antibodies N-NaPi2b in ovarian cancer cells
The sodium-dependent phosphate cotransporter NaPi2b encoded by the SLC34A2 gene is a transmembrane protein that belongs to the family of phosphate transporters SLC34 and is involved in maintaining phosphate homeostasis in the human body. The phosphate transporter NaPi2b is expressed in several norma...
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Kazan Federal University
2020-12-01
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| Series: | Учёные записки Казанского университета: Серия Естественные науки |
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| Online Access: | https://kpfu.ru/uz-eng-ns-2020-4-2.html |
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| author | L.F. Minigulova V.S. Skripova A.K. Nurgalieva D.V. Savenkova A.S. Kozlova N.I. Akberova D.D. Reshetnikova L.A. Savinska O.M. Garifulin M.V. Bogdanov R.G. Kiyamova |
| author_facet | L.F. Minigulova V.S. Skripova A.K. Nurgalieva D.V. Savenkova A.S. Kozlova N.I. Akberova D.D. Reshetnikova L.A. Savinska O.M. Garifulin M.V. Bogdanov R.G. Kiyamova |
| author_sort | L.F. Minigulova |
| collection | DOAJ |
| description | The sodium-dependent phosphate cotransporter NaPi2b encoded by the SLC34A2 gene is a transmembrane protein that belongs to the family of phosphate transporters SLC34 and is involved in maintaining phosphate homeostasis in the human body. The phosphate transporter NaPi2b is expressed in several normal tissues and body organs, as well as in malignant neoplasms of the ovary, lung, colorectal, and other types of cancer. The membrane protein NaPi2b is a target for therapeutic humanized antibodies Rebmab200, XMT-1535, and XMT-1536 in cancer cells. These antibodies were created based on MX35 antibodies and are directed against the large extracellular domain of the transporter NaPi2b. Since the location of the N-terminal domain of NaPi2b in the cell has not been experimentally confirmed, this work aimed to analyze the recognition of the phosphate transporter NaPi2b in ovarian cancer cells OVCAR-4 and OVCAR-8 by antibodies against the N-terminal domain of the transporter using Western blot analysis and confocal microscopy. |
| format | Article |
| id | doaj-art-1ddaef757e7248fcb9b3e8b05ea452b1 |
| institution | DOAJ |
| issn | 2542-064X 2500-218X |
| language | English |
| publishDate | 2020-12-01 |
| publisher | Kazan Federal University |
| record_format | Article |
| series | Учёные записки Казанского университета: Серия Естественные науки |
| spelling | doaj-art-1ddaef757e7248fcb9b3e8b05ea452b12025-08-20T02:58:30ZengKazan Federal UniversityУчёные записки Казанского университета: Серия Естественные науки2542-064X2500-218X2020-12-01162452954010.26907/2542-064X.2020.4.529-540Recognition of the sodium-dependent phosphate transporter NaPi2b by monoclonal antibodies N-NaPi2b in ovarian cancer cellsL.F. Minigulova0V.S. Skripova1A.K. Nurgalieva2D.V. Savenkova3A.S. Kozlova4N.I. Akberova5D.D. Reshetnikova6L.A. Savinska7O.M. Garifulin8M.V. Bogdanov9R.G. Kiyamova10Kazan Federal University, Kazan, 420008 RussiaKazan Federal University, Kazan, 420008 RussiaKazan Federal University, Kazan, 420008 RussiaKazan Federal University, Kazan, 420008 RussiaKazan Federal University, Kazan, 420008 RussiaKazan Federal University, Kazan, 420008 RussiaKazan Federal University, Kazan, 420008 RussiaInstitute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, Kiev, 03680 UkraineInstitute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, Kiev, 03680 UkraineKazan Federal University, Kazan, 420008 Russia; University of Texas Health Science Center at Houston, Houston, TX 77030 USAKazan Federal University, Kazan, 420008 RussiaThe sodium-dependent phosphate cotransporter NaPi2b encoded by the SLC34A2 gene is a transmembrane protein that belongs to the family of phosphate transporters SLC34 and is involved in maintaining phosphate homeostasis in the human body. The phosphate transporter NaPi2b is expressed in several normal tissues and body organs, as well as in malignant neoplasms of the ovary, lung, colorectal, and other types of cancer. The membrane protein NaPi2b is a target for therapeutic humanized antibodies Rebmab200, XMT-1535, and XMT-1536 in cancer cells. These antibodies were created based on MX35 antibodies and are directed against the large extracellular domain of the transporter NaPi2b. Since the location of the N-terminal domain of NaPi2b in the cell has not been experimentally confirmed, this work aimed to analyze the recognition of the phosphate transporter NaPi2b in ovarian cancer cells OVCAR-4 and OVCAR-8 by antibodies against the N-terminal domain of the transporter using Western blot analysis and confocal microscopy.https://kpfu.ru/uz-eng-ns-2020-4-2.htmlslc34a2napi2bn-terminal domain of napi2bmonoclonal antibodiesovarian cancer |
| spellingShingle | L.F. Minigulova V.S. Skripova A.K. Nurgalieva D.V. Savenkova A.S. Kozlova N.I. Akberova D.D. Reshetnikova L.A. Savinska O.M. Garifulin M.V. Bogdanov R.G. Kiyamova Recognition of the sodium-dependent phosphate transporter NaPi2b by monoclonal antibodies N-NaPi2b in ovarian cancer cells Учёные записки Казанского университета: Серия Естественные науки slc34a2 napi2b n-terminal domain of napi2b monoclonal antibodies ovarian cancer |
| title | Recognition of the sodium-dependent phosphate transporter NaPi2b by monoclonal antibodies N-NaPi2b in ovarian cancer cells |
| title_full | Recognition of the sodium-dependent phosphate transporter NaPi2b by monoclonal antibodies N-NaPi2b in ovarian cancer cells |
| title_fullStr | Recognition of the sodium-dependent phosphate transporter NaPi2b by monoclonal antibodies N-NaPi2b in ovarian cancer cells |
| title_full_unstemmed | Recognition of the sodium-dependent phosphate transporter NaPi2b by monoclonal antibodies N-NaPi2b in ovarian cancer cells |
| title_short | Recognition of the sodium-dependent phosphate transporter NaPi2b by monoclonal antibodies N-NaPi2b in ovarian cancer cells |
| title_sort | recognition of the sodium dependent phosphate transporter napi2b by monoclonal antibodies n napi2b in ovarian cancer cells |
| topic | slc34a2 napi2b n-terminal domain of napi2b monoclonal antibodies ovarian cancer |
| url | https://kpfu.ru/uz-eng-ns-2020-4-2.html |
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