Screening of Lignocellulose-Degrading Superior Mushroom Strains and Determination of Their CMCase and Laccase Activity
In order to screen lignocellulose-degrading superior mushroom strains ten strains of mushrooms (Lentinus edodes939, Pholiota nameko, Lentinus edodes868, Coprinus comatus, Macrolepiota procera, Auricularia auricula, Hericium erinaceus, Grifola frondosa, Pleurotus nebrodensis, and Shiraia bambusicola)...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2014/763108 |
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| author | Li Fen Zhu Xuwei Li Nanyi Zhang Puyu Zhang Shuang Zhao Xue Li Pengju Zhu Qichao Lin Haiping |
| author_facet | Li Fen Zhu Xuwei Li Nanyi Zhang Puyu Zhang Shuang Zhao Xue Li Pengju Zhu Qichao Lin Haiping |
| author_sort | Li Fen |
| collection | DOAJ |
| description | In order to screen lignocellulose-degrading superior mushroom strains ten strains of mushrooms (Lentinus edodes939, Pholiota nameko, Lentinus edodes868, Coprinus comatus, Macrolepiota procera, Auricularia auricula, Hericium erinaceus, Grifola frondosa, Pleurotus nebrodensis, and Shiraia bambusicola) were inoculated onto carboxymethylcellulose agar-Congo red plates to evaluate their ability to produce carbomethyl cellulase (CMCase). The results showed that the ratio of transparent circle to mycelium circle of Hericium erinaceus was 8.16 (P<0.01) higher than other strains. The filter paper culture screening test showed that Hericium erinaceus and Macrolepiota procera grew well and showed extreme decomposition of the filter paper. When cultivated in guaiacol culture medium to detect their abilities to secrete laccase, Hericium erinaceus showed the highest ability with the largest reddish brown circles of 4.330 cm. CMCase activity determination indicated that Coprinus comatus and Hericium erinaceus had the ability to produce CMCase with 33.92 U/L on the 9th day and 22.58 U/L on the 10th day, respectively, while Coprinus comatus and Pleurotus nebrodensis had the ability to produce laccase with 496.67 U/L and 489.17 U/L on the 16th day and 18th day. Based on the results, Coprinus comatus might be the most promising lignocellulose-degrading strain to produce both CMCase and laccase at high levels. |
| format | Article |
| id | doaj-art-1dd66c1542454ebbbdae9fbb39937dc6 |
| institution | Kabale University |
| issn | 2356-6140 1537-744X |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-1dd66c1542454ebbbdae9fbb39937dc62025-02-03T06:07:34ZengWileyThe Scientific World Journal2356-61401537-744X2014-01-01201410.1155/2014/763108763108Screening of Lignocellulose-Degrading Superior Mushroom Strains and Determination of Their CMCase and Laccase ActivityLi Fen0Zhu Xuwei1Li Nanyi2Zhang Puyu3Zhang Shuang4Zhao Xue5Li Pengju6Zhu Qichao7Lin Haiping8The Nurturing Station for the State Key Laboratory of Subtropical Silviculture, Provincial Engineering Laboratory of Biopesticide Preparation, Zhejiang A&F University, Lin’an 311300, ChinaThe Nurturing Station for the State Key Laboratory of Subtropical Silviculture, Provincial Engineering Laboratory of Biopesticide Preparation, Zhejiang A&F University, Lin’an 311300, ChinaSchool of Agricultural and Food Science, Zhejiang A&F University, Lin’an 311300, ChinaThe Nurturing Station for the State Key Laboratory of Subtropical Silviculture, Provincial Engineering Laboratory of Biopesticide Preparation, Zhejiang A&F University, Lin’an 311300, ChinaThe Nurturing Station for the State Key Laboratory of Subtropical Silviculture, Provincial Engineering Laboratory of Biopesticide Preparation, Zhejiang A&F University, Lin’an 311300, ChinaThe Nurturing Station for the State Key Laboratory of Subtropical Silviculture, Provincial Engineering Laboratory of Biopesticide Preparation, Zhejiang A&F University, Lin’an 311300, ChinaThe Nurturing Station for the State Key Laboratory of Subtropical Silviculture, Provincial Engineering Laboratory of Biopesticide Preparation, Zhejiang A&F University, Lin’an 311300, ChinaQingyuan Forestry Bureau of Zhejiang, Qingyuan 323800, ChinaThe Nurturing Station for the State Key Laboratory of Subtropical Silviculture, Provincial Engineering Laboratory of Biopesticide Preparation, Zhejiang A&F University, Lin’an 311300, ChinaIn order to screen lignocellulose-degrading superior mushroom strains ten strains of mushrooms (Lentinus edodes939, Pholiota nameko, Lentinus edodes868, Coprinus comatus, Macrolepiota procera, Auricularia auricula, Hericium erinaceus, Grifola frondosa, Pleurotus nebrodensis, and Shiraia bambusicola) were inoculated onto carboxymethylcellulose agar-Congo red plates to evaluate their ability to produce carbomethyl cellulase (CMCase). The results showed that the ratio of transparent circle to mycelium circle of Hericium erinaceus was 8.16 (P<0.01) higher than other strains. The filter paper culture screening test showed that Hericium erinaceus and Macrolepiota procera grew well and showed extreme decomposition of the filter paper. When cultivated in guaiacol culture medium to detect their abilities to secrete laccase, Hericium erinaceus showed the highest ability with the largest reddish brown circles of 4.330 cm. CMCase activity determination indicated that Coprinus comatus and Hericium erinaceus had the ability to produce CMCase with 33.92 U/L on the 9th day and 22.58 U/L on the 10th day, respectively, while Coprinus comatus and Pleurotus nebrodensis had the ability to produce laccase with 496.67 U/L and 489.17 U/L on the 16th day and 18th day. Based on the results, Coprinus comatus might be the most promising lignocellulose-degrading strain to produce both CMCase and laccase at high levels.http://dx.doi.org/10.1155/2014/763108 |
| spellingShingle | Li Fen Zhu Xuwei Li Nanyi Zhang Puyu Zhang Shuang Zhao Xue Li Pengju Zhu Qichao Lin Haiping Screening of Lignocellulose-Degrading Superior Mushroom Strains and Determination of Their CMCase and Laccase Activity The Scientific World Journal |
| title | Screening of Lignocellulose-Degrading Superior Mushroom Strains and Determination of Their CMCase and Laccase Activity |
| title_full | Screening of Lignocellulose-Degrading Superior Mushroom Strains and Determination of Their CMCase and Laccase Activity |
| title_fullStr | Screening of Lignocellulose-Degrading Superior Mushroom Strains and Determination of Their CMCase and Laccase Activity |
| title_full_unstemmed | Screening of Lignocellulose-Degrading Superior Mushroom Strains and Determination of Their CMCase and Laccase Activity |
| title_short | Screening of Lignocellulose-Degrading Superior Mushroom Strains and Determination of Their CMCase and Laccase Activity |
| title_sort | screening of lignocellulose degrading superior mushroom strains and determination of their cmcase and laccase activity |
| url | http://dx.doi.org/10.1155/2014/763108 |
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