Setting of graded levels of a protein in yeast by a t-degron technique as applied to phosphoglycerate mutase
Abstract Background Setting of graded levels of a protein for in vivo studies by controlled gene expression has inconveniences, and we here explore the use of the t-degron technique instead. Results In a yeast t-degron (ubiquitin-argDHFRts)- phosphoglycerate mutase (GPM1) fusion strain, increasing p...
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| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2002-07-01
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| Series: | BMC Genetics |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/1471-2156-3-39 |
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