Viromics-based precision diagnosis of reproductive abnormalities in cows reveals a reassortant Akabane disease virus
Abstract Background At the end of 2021, an epidemic of reproductive abnormalities in cows occurred in Jilin Province, China, posing an urgent need for a rapid diagnosis. Results To identify the cause of the disease, a total of 172 samples were collected from 21 dead calves and 45 aborting or pregnan...
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| Language: | English |
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BMC
2024-11-01
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| Series: | BMC Veterinary Research |
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| Online Access: | https://doi.org/10.1186/s12917-024-04400-5 |
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| author | Yue Sun Ru Zhang Huiyu Wang Zheng Sun Le Yi Changchun Tu Yanling Yang Biao He |
| author_facet | Yue Sun Ru Zhang Huiyu Wang Zheng Sun Le Yi Changchun Tu Yanling Yang Biao He |
| author_sort | Yue Sun |
| collection | DOAJ |
| description | Abstract Background At the end of 2021, an epidemic of reproductive abnormalities in cows occurred in Jilin Province, China, posing an urgent need for a rapid diagnosis. Results To identify the cause of the disease, a total of 172 samples were collected from 21 dead calves and 45 aborting or pregnant cows in 10 farms across the province. Routine PCR or RT-PCR detection did not find any common abortion-related agents. We then employed the viromics-based precision diagnosis method to analyze these samples, and the read-based annotation showed signals of an Akabane disease virus (AKAV) in some libraries. To further identify the virus, nested RT-PCR detection revealed that 52.3% (11/21) of dead calves and 26.6% (12/45) of cows were positive for the virus. Phylogenetic analysis of the partial fragments showed that the S segment of the virus was 100% identical to the Chinese strain TJ2016, but its M and L segments shared 94.3% and 96.5% identities with an Israeli strain. Conclusions The viromic and molecular results suggested that these animals were infected with a reassortant AKAV. Coupled with the clinical signs, the virus should be responsible for the epizootic, highlighting that molecular and serological surveys of the virus in cows during early pregnancy, as well as ecological investigation in its arthropod vectors, are necessary. |
| format | Article |
| id | doaj-art-1dad4a21207b45809f5c1f1ce7c9cd0e |
| institution | OA Journals |
| issn | 1746-6148 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Veterinary Research |
| spelling | doaj-art-1dad4a21207b45809f5c1f1ce7c9cd0e2025-08-20T02:08:16ZengBMCBMC Veterinary Research1746-61482024-11-012011910.1186/s12917-024-04400-5Viromics-based precision diagnosis of reproductive abnormalities in cows reveals a reassortant Akabane disease virusYue Sun0Ru Zhang1Huiyu Wang2Zheng Sun3Le Yi4Changchun Tu5Yanling Yang6Biao He7Changchun Veterinary Research Institute, Chinese Academy of Agricultural SciencesInstitute of Special Animal and Plant Sciences, Chinese Academy of Agricultural SciencesChangchun Veterinary Research Institute, Chinese Academy of Agricultural SciencesChangchun Veterinary Research Institute, Chinese Academy of Agricultural SciencesChangchun Veterinary Research Institute, Chinese Academy of Agricultural SciencesChangchun Veterinary Research Institute, Chinese Academy of Agricultural SciencesInstitute of Special Animal and Plant Sciences, Chinese Academy of Agricultural SciencesChangchun Veterinary Research Institute, Chinese Academy of Agricultural SciencesAbstract Background At the end of 2021, an epidemic of reproductive abnormalities in cows occurred in Jilin Province, China, posing an urgent need for a rapid diagnosis. Results To identify the cause of the disease, a total of 172 samples were collected from 21 dead calves and 45 aborting or pregnant cows in 10 farms across the province. Routine PCR or RT-PCR detection did not find any common abortion-related agents. We then employed the viromics-based precision diagnosis method to analyze these samples, and the read-based annotation showed signals of an Akabane disease virus (AKAV) in some libraries. To further identify the virus, nested RT-PCR detection revealed that 52.3% (11/21) of dead calves and 26.6% (12/45) of cows were positive for the virus. Phylogenetic analysis of the partial fragments showed that the S segment of the virus was 100% identical to the Chinese strain TJ2016, but its M and L segments shared 94.3% and 96.5% identities with an Israeli strain. Conclusions The viromic and molecular results suggested that these animals were infected with a reassortant AKAV. Coupled with the clinical signs, the virus should be responsible for the epizootic, highlighting that molecular and serological surveys of the virus in cows during early pregnancy, as well as ecological investigation in its arthropod vectors, are necessary.https://doi.org/10.1186/s12917-024-04400-5ViromeAkabane disease virusArbovirusCattleReassortment |
| spellingShingle | Yue Sun Ru Zhang Huiyu Wang Zheng Sun Le Yi Changchun Tu Yanling Yang Biao He Viromics-based precision diagnosis of reproductive abnormalities in cows reveals a reassortant Akabane disease virus BMC Veterinary Research Virome Akabane disease virus Arbovirus Cattle Reassortment |
| title | Viromics-based precision diagnosis of reproductive abnormalities in cows reveals a reassortant Akabane disease virus |
| title_full | Viromics-based precision diagnosis of reproductive abnormalities in cows reveals a reassortant Akabane disease virus |
| title_fullStr | Viromics-based precision diagnosis of reproductive abnormalities in cows reveals a reassortant Akabane disease virus |
| title_full_unstemmed | Viromics-based precision diagnosis of reproductive abnormalities in cows reveals a reassortant Akabane disease virus |
| title_short | Viromics-based precision diagnosis of reproductive abnormalities in cows reveals a reassortant Akabane disease virus |
| title_sort | viromics based precision diagnosis of reproductive abnormalities in cows reveals a reassortant akabane disease virus |
| topic | Virome Akabane disease virus Arbovirus Cattle Reassortment |
| url | https://doi.org/10.1186/s12917-024-04400-5 |
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