Comparative Performance Evaluation of Continuous Monitoring Blood Culture Systems Using Simulated Septic Specimen
<b>Background/Objectives:</b> Continuous monitoring blood culture systems (CMBCSs) are revolutionary automated instruments that facilitate the rapid identification of pathogens in blood samples from patients with sepsis. However, with only a few CMBCSs being widely used as references, us...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-02-01
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| Series: | Diagnostics |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2075-4418/15/4/468 |
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| Summary: | <b>Background/Objectives:</b> Continuous monitoring blood culture systems (CMBCSs) are revolutionary automated instruments that facilitate the rapid identification of pathogens in blood samples from patients with sepsis. However, with only a few CMBCSs being widely used as references, user dependency on these limited options has grown. In response, a new CMBCS was developed and compared with existing systems to evaluate microbial growth. <b>Methods:</b> HubCentra84 was compared to BacT/Alert<sup>®</sup> 3D and BACTEC™ FX. <i>Staphylococcus aureus</i>, <i>Streptococcus pneumoniae</i>, <i>Escherichia coli</i>, <i>Pseudomonas aeruginosa</i>, <i>Bacteroides fragilis</i>, and <i>Candida albicans</i> were selected as representative clinically infectious microorganisms. Colonies from pure cultures were diluted with 0.9% saline to create simulated sepsis specimens (SSSs). The SSSs were injected into dedicated culture bottles for each instrument. Thirty paired tests were performed for each strain. <b>Results:</b> Colony-forming units of the added SSSs were consistent according to bacteria, and all strains demonstrated robust growth in three CMBCSs. Time-to-positivity was uniformly observed according to the instruments used. The novel CMBCS detected the growth of the clinically significant bacteria <i>S. aureus</i>, <i>S. pneumoniae</i>, <i>E. coli</i>, and <i>P. aeruginosa</i> approximately 2 h faster than the other two systems. However, it was approximately 200 min slower for <i>C. albicans</i> and 3000 min for <i>B. fragilis</i>. <b>Conclusions:</b> The novel CMBCS demonstrates advantages in detecting the growth of common clinical bacteria. Although slow growth was detected for certain microorganisms, it successfully captured the growth of all tested microorganisms. |
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| ISSN: | 2075-4418 |