Value of collaborative investigation by hospital infection control, public health services and a national reference laboratory during an increase in puerperal sepsis

Abstract Background In a Dutch tertiary care hospital, two cases of puerperal sepsis were diagnosed within 16 days in June-July 2022. The subsequent outbreak investigation emphasizes the value of collaboration between hospital infection control, regional public health services (PHS) and a national r...

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Main Authors: Irene V. Hoogendijk, Diane de Zwart - Slats, Stefan A. Boers, Boas C.L. van der Putten, Nina M. van Sorge, Bibi D.H. Molenaar, Myrthe M.A. Toorop, Marieke B. Veenhof, Karin Ellen Veldkamp, Adriënne S. van der Schoor, Joffrey van Prehn
Format: Article
Language:English
Published: BMC 2025-05-01
Series:Antimicrobial Resistance and Infection Control
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Online Access:https://doi.org/10.1186/s13756-025-01564-z
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author Irene V. Hoogendijk
Diane de Zwart - Slats
Stefan A. Boers
Boas C.L. van der Putten
Nina M. van Sorge
Bibi D.H. Molenaar
Myrthe M.A. Toorop
Marieke B. Veenhof
Karin Ellen Veldkamp
Adriënne S. van der Schoor
Joffrey van Prehn
author_facet Irene V. Hoogendijk
Diane de Zwart - Slats
Stefan A. Boers
Boas C.L. van der Putten
Nina M. van Sorge
Bibi D.H. Molenaar
Myrthe M.A. Toorop
Marieke B. Veenhof
Karin Ellen Veldkamp
Adriënne S. van der Schoor
Joffrey van Prehn
author_sort Irene V. Hoogendijk
collection DOAJ
description Abstract Background In a Dutch tertiary care hospital, two cases of puerperal sepsis were diagnosed within 16 days in June-July 2022. The subsequent outbreak investigation emphasizes the value of collaboration between hospital infection control, regional public health services (PHS) and a national reference laboratory. The aim was to identify possible causes of this increase to prevent further cases of puerperal sepsis. Methods Hospital infection control identified a group of puerperal sepsis cases clustered within the last year in the hospital, a cluster caused by S. pyogenes emm12.0. The hospital and PHS performed contact tracing of cases and HCW involved, investigating epidemiological links, and screening of HCW. The Netherlands Reference Laboratory for Bacterial Meningitis (NRLBM) identified additional regional cases. Subsequently, whole genome sequencing (WGS) analysis was performed on clinical, HCW and regional S. pyogenes isolates. Results Four maternity ward patients were diagnosed with puerperal sepsis caused by S. pyogenes emm12.0 between April and November 2022. Although no additional epidemiological links were identified, all four cases resided within a 6.6 km radius. WGS analysis showed that the four cases were part of an 11-case cluster. Screening of HCW (n = 197) identified two individuals carrying clonally related S. pyogenes isolates. Conclusions Collaboration between hospital, PHS, and NRLBM resulted in an overview of possible epidemiological links. Centralized collection of iGAS case information and strain typing are critical to place hospital clusters in the context of local epidemiology. An increase in healthcare-associated infections may not necessarily imply in-hospital transmission.
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spelling doaj-art-1d9da737912f4b99a6f313016efd5ac02025-08-20T03:16:31ZengBMCAntimicrobial Resistance and Infection Control2047-29942025-05-011411710.1186/s13756-025-01564-zValue of collaborative investigation by hospital infection control, public health services and a national reference laboratory during an increase in puerperal sepsisIrene V. Hoogendijk0Diane de Zwart - Slats1Stefan A. Boers2Boas C.L. van der Putten3Nina M. van Sorge4Bibi D.H. Molenaar5Myrthe M.A. Toorop6Marieke B. Veenhof7Karin Ellen Veldkamp8Adriënne S. van der Schoor9Joffrey van Prehn10Department of Medical Microbiology and Infection Prevention, Centre for Infectious Diseases (LU-CID), Leiden University, Leiden University Medical CentreDepartment of infectious disease, Public Health Service region Hollands MiddenDepartment of Medical Microbiology and Infection Prevention, Centre for Infectious Diseases (LU-CID), Leiden University, Leiden University Medical CentreDepartment of Medical Microbiology and Infection Prevention, Netherlands Reference Laboratory for Bacterial Meningitis, Amsterdam University Medical CentreDepartment of Medical Microbiology and Infection Prevention, Netherlands Reference Laboratory for Bacterial Meningitis, Amsterdam University Medical CentreDepartment of Occupational Medicine, Leiden University Medical CentreDepartment of Medical Microbiology and Infection Prevention, Centre for Infectious Diseases (LU-CID), Leiden University, Leiden University Medical CentreDepartment of Obstetrics, Leiden University Medical CentreDepartment of Medical Microbiology and Infection Prevention, Centre for Infectious Diseases (LU-CID), Leiden University, Leiden University Medical CentreDepartment of infectious disease, Public Health Service region Hollands MiddenDepartment of Medical Microbiology and Infection Prevention, Centre for Infectious Diseases (LU-CID), Leiden University, Leiden University Medical CentreAbstract Background In a Dutch tertiary care hospital, two cases of puerperal sepsis were diagnosed within 16 days in June-July 2022. The subsequent outbreak investigation emphasizes the value of collaboration between hospital infection control, regional public health services (PHS) and a national reference laboratory. The aim was to identify possible causes of this increase to prevent further cases of puerperal sepsis. Methods Hospital infection control identified a group of puerperal sepsis cases clustered within the last year in the hospital, a cluster caused by S. pyogenes emm12.0. The hospital and PHS performed contact tracing of cases and HCW involved, investigating epidemiological links, and screening of HCW. The Netherlands Reference Laboratory for Bacterial Meningitis (NRLBM) identified additional regional cases. Subsequently, whole genome sequencing (WGS) analysis was performed on clinical, HCW and regional S. pyogenes isolates. Results Four maternity ward patients were diagnosed with puerperal sepsis caused by S. pyogenes emm12.0 between April and November 2022. Although no additional epidemiological links were identified, all four cases resided within a 6.6 km radius. WGS analysis showed that the four cases were part of an 11-case cluster. Screening of HCW (n = 197) identified two individuals carrying clonally related S. pyogenes isolates. Conclusions Collaboration between hospital, PHS, and NRLBM resulted in an overview of possible epidemiological links. Centralized collection of iGAS case information and strain typing are critical to place hospital clusters in the context of local epidemiology. An increase in healthcare-associated infections may not necessarily imply in-hospital transmission.https://doi.org/10.1186/s13756-025-01564-zS. pyogenesGroup A StreptococcusInfectionPuerperal sepsisMolecular typingWhole-Genome sequencing
spellingShingle Irene V. Hoogendijk
Diane de Zwart - Slats
Stefan A. Boers
Boas C.L. van der Putten
Nina M. van Sorge
Bibi D.H. Molenaar
Myrthe M.A. Toorop
Marieke B. Veenhof
Karin Ellen Veldkamp
Adriënne S. van der Schoor
Joffrey van Prehn
Value of collaborative investigation by hospital infection control, public health services and a national reference laboratory during an increase in puerperal sepsis
Antimicrobial Resistance and Infection Control
S. pyogenes
Group A Streptococcus
Infection
Puerperal sepsis
Molecular typing
Whole-Genome sequencing
title Value of collaborative investigation by hospital infection control, public health services and a national reference laboratory during an increase in puerperal sepsis
title_full Value of collaborative investigation by hospital infection control, public health services and a national reference laboratory during an increase in puerperal sepsis
title_fullStr Value of collaborative investigation by hospital infection control, public health services and a national reference laboratory during an increase in puerperal sepsis
title_full_unstemmed Value of collaborative investigation by hospital infection control, public health services and a national reference laboratory during an increase in puerperal sepsis
title_short Value of collaborative investigation by hospital infection control, public health services and a national reference laboratory during an increase in puerperal sepsis
title_sort value of collaborative investigation by hospital infection control public health services and a national reference laboratory during an increase in puerperal sepsis
topic S. pyogenes
Group A Streptococcus
Infection
Puerperal sepsis
Molecular typing
Whole-Genome sequencing
url https://doi.org/10.1186/s13756-025-01564-z
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