CD63 Promotes Hemocyte-Mediated Phagocytosis in the Clam, Paphia undulata
As one of the surface membrane proteins of tetraspanin family, CD63 plays a crucial role in cellular trafficking and endocytosis, which also is associated with activation of a wide variety of immune cells. Here, the homolog of CD63 was characterized from one marine mollusk, Paphia undulata, which is...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2016/7893490 |
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| author | Mingjia Yu Shanjun Yang Hongxia Sun Qiang Xia |
| author_facet | Mingjia Yu Shanjun Yang Hongxia Sun Qiang Xia |
| author_sort | Mingjia Yu |
| collection | DOAJ |
| description | As one of the surface membrane proteins of tetraspanin family, CD63 plays a crucial role in cellular trafficking and endocytosis, which also is associated with activation of a wide variety of immune cells. Here, the homolog of CD63 was characterized from one marine mollusk, Paphia undulata, which is designated as Pu-CD63. The complete cDNA of Pu-CD63 is 1,738 bp in length with an open reading frame (ORF) of 849 bp, encoding a 282 amino acid protein with four putative hydrophobic transmembrane helixes. Bioinformatic analysis revealed that Pu-CD63 contains one putative YXXØ consensus motif of “110-YVII-113” and one N-glycosylation site “155-NGT-157” within the large extracellular loop (LEL) region, supporting its conserved function in plasma membrane and endosomal/lysosomal trafficking. Moreover, temporal expression profile analysis demonstrates a drastic induction in the expression of CD63 in hemocytes after pathogenic challenge with either V. parahaemolyticus or V. alginolyticus. By performing dsRNA-mediate RNAi knockdowns of CD63, a dramatic reduction in hemocytes phagocytic activity to pathogenic Vibrio is recorded by flow cytometry, revealing the definite role of Pu-CD63 in promoting hemocyte-mediated phagocytosis. Therefore, our work has greatly enhanced our understanding about primitive character of innate immunity in marine mollusk. |
| format | Article |
| id | doaj-art-1d876d570cbb476884be6f6d57c98f0b |
| institution | Kabale University |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-1d876d570cbb476884be6f6d57c98f0b2025-02-03T05:48:27ZengWileyJournal of Immunology Research2314-88612314-71562016-01-01201610.1155/2016/78934907893490CD63 Promotes Hemocyte-Mediated Phagocytosis in the Clam, Paphia undulataMingjia Yu0Shanjun Yang1Hongxia Sun2Qiang Xia3Engineering Research Center of Marine Biological Resource Comprehensive Utilization, Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, ChinaEngineering Research Center of Marine Biological Resource Comprehensive Utilization, Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, ChinaDepartment of Immunology and Pathogen Biology, Zunyi Medical College, Zhuhai Campus, Zhuhai 519041, ChinaDepartment of Immunology and Pathogen Biology, Zunyi Medical College, Zhuhai Campus, Zhuhai 519041, ChinaAs one of the surface membrane proteins of tetraspanin family, CD63 plays a crucial role in cellular trafficking and endocytosis, which also is associated with activation of a wide variety of immune cells. Here, the homolog of CD63 was characterized from one marine mollusk, Paphia undulata, which is designated as Pu-CD63. The complete cDNA of Pu-CD63 is 1,738 bp in length with an open reading frame (ORF) of 849 bp, encoding a 282 amino acid protein with four putative hydrophobic transmembrane helixes. Bioinformatic analysis revealed that Pu-CD63 contains one putative YXXØ consensus motif of “110-YVII-113” and one N-glycosylation site “155-NGT-157” within the large extracellular loop (LEL) region, supporting its conserved function in plasma membrane and endosomal/lysosomal trafficking. Moreover, temporal expression profile analysis demonstrates a drastic induction in the expression of CD63 in hemocytes after pathogenic challenge with either V. parahaemolyticus or V. alginolyticus. By performing dsRNA-mediate RNAi knockdowns of CD63, a dramatic reduction in hemocytes phagocytic activity to pathogenic Vibrio is recorded by flow cytometry, revealing the definite role of Pu-CD63 in promoting hemocyte-mediated phagocytosis. Therefore, our work has greatly enhanced our understanding about primitive character of innate immunity in marine mollusk.http://dx.doi.org/10.1155/2016/7893490 |
| spellingShingle | Mingjia Yu Shanjun Yang Hongxia Sun Qiang Xia CD63 Promotes Hemocyte-Mediated Phagocytosis in the Clam, Paphia undulata Journal of Immunology Research |
| title | CD63 Promotes Hemocyte-Mediated Phagocytosis in the Clam, Paphia undulata |
| title_full | CD63 Promotes Hemocyte-Mediated Phagocytosis in the Clam, Paphia undulata |
| title_fullStr | CD63 Promotes Hemocyte-Mediated Phagocytosis in the Clam, Paphia undulata |
| title_full_unstemmed | CD63 Promotes Hemocyte-Mediated Phagocytosis in the Clam, Paphia undulata |
| title_short | CD63 Promotes Hemocyte-Mediated Phagocytosis in the Clam, Paphia undulata |
| title_sort | cd63 promotes hemocyte mediated phagocytosis in the clam paphia undulata |
| url | http://dx.doi.org/10.1155/2016/7893490 |
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