Robust Controlled Degradation of Enzyme Loaded PCL‐Based Fibrous Scaffolds Toward Scarless Skin Tissue Regeneration
Abstract Uncontrolled degradation of wound dressings may result in residues, causing several negative effects on wound healing, such as secondary damage, undesirable inflammation, and scar skin formation. Here, an available strategy associated with the synthesis of enzyme‐loaded (Burkholderia cepaci...
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Wiley
2025-07-01
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| Series: | Advanced Science |
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| Online Access: | https://doi.org/10.1002/advs.202501053 |
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| author | Lingling Fan Weiliang Dong Jianqi Lu Yujia Peng Bin Xie Ping Wei Min Jiang Su Chen |
| author_facet | Lingling Fan Weiliang Dong Jianqi Lu Yujia Peng Bin Xie Ping Wei Min Jiang Su Chen |
| author_sort | Lingling Fan |
| collection | DOAJ |
| description | Abstract Uncontrolled degradation of wound dressings may result in residues, causing several negative effects on wound healing, such as secondary damage, undesirable inflammation, and scar skin formation. Here, an available strategy associated with the synthesis of enzyme‐loaded (Burkholderia cepacia lipase, BCL) polycaprolactone (PCL) nanofiber scaffolds, aligning with wound healing effects is reported. These scaffolds are fabricated via fiber microfluidic electrospinning degradation‐control technique. The obtained scaffolds exhibit tunable degradation rates, achieving complete degradation within 12–72‐h cycles. The acidic degradation products are further elucidated and reveal the potential degradation mechanism. The acidic degradation products create an optimal microenvironment during the hemostasis and inflammation stages of wound healing. Notably, in vivo experiments demonstrate the enzyme‐loaded scaffolds effectively promote angiogenesis, reduce inflammatory responses, mitigate collagen deposition, and regulate fibroblast differentiation. This promotes rapid wound healing with a remarkable scarless rate of over 99% by day 21. New guidelines for scar‐free healing dressings are proposed, which carry out faster degradation without microplastics (MPs) and toxic byproducts before scar formation. These principles might provide valuable insights and promise for developing more effective wound dressings. |
| format | Article |
| id | doaj-art-1d86112ce6eb42cda55336250a8346d9 |
| institution | DOAJ |
| issn | 2198-3844 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Wiley |
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| series | Advanced Science |
| spelling | doaj-art-1d86112ce6eb42cda55336250a8346d92025-08-20T03:15:35ZengWileyAdvanced Science2198-38442025-07-011225n/an/a10.1002/advs.202501053Robust Controlled Degradation of Enzyme Loaded PCL‐Based Fibrous Scaffolds Toward Scarless Skin Tissue RegenerationLingling Fan0Weiliang Dong1Jianqi Lu2Yujia Peng3Bin Xie4Ping Wei5Min Jiang6Su Chen7Key Laboratory for Waste Plastics Biocatalytic Degradation and RecyclingCollege of Biotechnology and Pharmaceutical EngineeringNanjing Tech UniversityNanjing 211816 ChinaKey Laboratory for Waste Plastics Biocatalytic Degradation and RecyclingCollege of Biotechnology and Pharmaceutical EngineeringNanjing Tech UniversityNanjing 211816 ChinaKey Laboratory for Waste Plastics Biocatalytic Degradation and RecyclingCollege of Biotechnology and Pharmaceutical EngineeringNanjing Tech UniversityNanjing 211816 ChinaKey Laboratory for Waste Plastics Biocatalytic Degradation and RecyclingCollege of Biotechnology and Pharmaceutical EngineeringNanjing Tech UniversityNanjing 211816 ChinaKey Laboratory for Waste Plastics Biocatalytic Degradation and RecyclingCollege of Biotechnology and Pharmaceutical EngineeringNanjing Tech UniversityNanjing 211816 ChinaKey Laboratory for Waste Plastics Biocatalytic Degradation and RecyclingCollege of Biotechnology and Pharmaceutical EngineeringNanjing Tech UniversityNanjing 211816 ChinaKey Laboratory for Waste Plastics Biocatalytic Degradation and RecyclingCollege of Biotechnology and Pharmaceutical EngineeringNanjing Tech UniversityNanjing 211816 ChinaState Key Laboratory of Materials‐Oriented Chemical EngineeringNanjing Tech UniversityNanjing 211816 ChinaAbstract Uncontrolled degradation of wound dressings may result in residues, causing several negative effects on wound healing, such as secondary damage, undesirable inflammation, and scar skin formation. Here, an available strategy associated with the synthesis of enzyme‐loaded (Burkholderia cepacia lipase, BCL) polycaprolactone (PCL) nanofiber scaffolds, aligning with wound healing effects is reported. These scaffolds are fabricated via fiber microfluidic electrospinning degradation‐control technique. The obtained scaffolds exhibit tunable degradation rates, achieving complete degradation within 12–72‐h cycles. The acidic degradation products are further elucidated and reveal the potential degradation mechanism. The acidic degradation products create an optimal microenvironment during the hemostasis and inflammation stages of wound healing. Notably, in vivo experiments demonstrate the enzyme‐loaded scaffolds effectively promote angiogenesis, reduce inflammatory responses, mitigate collagen deposition, and regulate fibroblast differentiation. This promotes rapid wound healing with a remarkable scarless rate of over 99% by day 21. New guidelines for scar‐free healing dressings are proposed, which carry out faster degradation without microplastics (MPs) and toxic byproducts before scar formation. These principles might provide valuable insights and promise for developing more effective wound dressings.https://doi.org/10.1002/advs.202501053degradation‐control fibrous scaffoldsenzyme‐loaded wound dressingsmicroplasticsnon‐toxic by‐productsscarless skin tissue regeneration |
| spellingShingle | Lingling Fan Weiliang Dong Jianqi Lu Yujia Peng Bin Xie Ping Wei Min Jiang Su Chen Robust Controlled Degradation of Enzyme Loaded PCL‐Based Fibrous Scaffolds Toward Scarless Skin Tissue Regeneration Advanced Science degradation‐control fibrous scaffolds enzyme‐loaded wound dressings microplastics non‐toxic by‐products scarless skin tissue regeneration |
| title | Robust Controlled Degradation of Enzyme Loaded PCL‐Based Fibrous Scaffolds Toward Scarless Skin Tissue Regeneration |
| title_full | Robust Controlled Degradation of Enzyme Loaded PCL‐Based Fibrous Scaffolds Toward Scarless Skin Tissue Regeneration |
| title_fullStr | Robust Controlled Degradation of Enzyme Loaded PCL‐Based Fibrous Scaffolds Toward Scarless Skin Tissue Regeneration |
| title_full_unstemmed | Robust Controlled Degradation of Enzyme Loaded PCL‐Based Fibrous Scaffolds Toward Scarless Skin Tissue Regeneration |
| title_short | Robust Controlled Degradation of Enzyme Loaded PCL‐Based Fibrous Scaffolds Toward Scarless Skin Tissue Regeneration |
| title_sort | robust controlled degradation of enzyme loaded pcl based fibrous scaffolds toward scarless skin tissue regeneration |
| topic | degradation‐control fibrous scaffolds enzyme‐loaded wound dressings microplastics non‐toxic by‐products scarless skin tissue regeneration |
| url | https://doi.org/10.1002/advs.202501053 |
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