Auxin-Producing Bacteria Used as Microbial Biostimulants Improve the Growth of Tomato (<i>Solanum lycopersicum</i> L.) Seedlings in Hydroponic Systems
Seven auxin-producing endophytic bacterial strains (<i>Azospirillum</i> spp., <i>Methylobacterium symbioticum</i>, <i>Bacillus</i> spp.), and two different combinations of these strains were used to verify their influence on tomato during germination and developme...
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2024-08-01
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| author | Livia Pappalettere Susanna Bartolini Annita Toffanin |
| author_facet | Livia Pappalettere Susanna Bartolini Annita Toffanin |
| author_sort | Livia Pappalettere |
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| description | Seven auxin-producing endophytic bacterial strains (<i>Azospirillum</i> spp., <i>Methylobacterium symbioticum</i>, <i>Bacillus</i> spp.), and two different combinations of these strains were used to verify their influence on tomato during germination and development in hydroponic conditions where, as a novelty for Canestrino di Lucca cultivar, endophytic bacteria were inoculated. To emphasize the presence of bacterial auxins in roots and stems of seedlings, both in situ staining qualitative assessment and quantitative analysis were carried out. Moreover, hypogeal and epigeal growth of the plantlets were measured, and correlation analyses were conducted to examine the relationship between the amount of indolacetic acid (IAA) produced by the bacterial strains and root and stem parameters. Plantlets treated with microbial inoculants showed a significant increase in the survival rate compared to the control treatment. The best results as IAA producers were from <i>Azospirillum baldaniorum</i> Sp245 and <i>A. brasilense</i> Cd, which also induced significant root growth. On the other hand, <i>Bacillus amyloliquefaciens</i> and <i>B. licheniformis</i> induced the best rates in stem growth. These findings highlight the potential for using endophytic bacterial strains in a hydroponic co-cultivation system that enables inoculating plantlets, at an early stage of growth (5 days old). |
| format | Article |
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| language | English |
| publishDate | 2024-08-01 |
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| spelling | doaj-art-1d7f38dd2e104fc3b9466bf375817a9e2025-08-20T01:56:11ZengMDPI AGBioTech2673-62842024-08-011333210.3390/biotech13030032Auxin-Producing Bacteria Used as Microbial Biostimulants Improve the Growth of Tomato (<i>Solanum lycopersicum</i> L.) Seedlings in Hydroponic SystemsLivia Pappalettere0Susanna Bartolini1Annita Toffanin2Institute of Crop Science, Sant’Anna School of Advanced Studies, Piazza Martiri della Libertà 33, 56127 Pisa, ItalyInstitute of Crop Science, Sant’Anna School of Advanced Studies, Piazza Martiri della Libertà 33, 56127 Pisa, ItalyDepartment of Agriculture Food and Environment (DAFE), University of Pisa, Via del Borghetto 80, 56124 Pisa, ItalySeven auxin-producing endophytic bacterial strains (<i>Azospirillum</i> spp., <i>Methylobacterium symbioticum</i>, <i>Bacillus</i> spp.), and two different combinations of these strains were used to verify their influence on tomato during germination and development in hydroponic conditions where, as a novelty for Canestrino di Lucca cultivar, endophytic bacteria were inoculated. To emphasize the presence of bacterial auxins in roots and stems of seedlings, both in situ staining qualitative assessment and quantitative analysis were carried out. Moreover, hypogeal and epigeal growth of the plantlets were measured, and correlation analyses were conducted to examine the relationship between the amount of indolacetic acid (IAA) produced by the bacterial strains and root and stem parameters. Plantlets treated with microbial inoculants showed a significant increase in the survival rate compared to the control treatment. The best results as IAA producers were from <i>Azospirillum baldaniorum</i> Sp245 and <i>A. brasilense</i> Cd, which also induced significant root growth. On the other hand, <i>Bacillus amyloliquefaciens</i> and <i>B. licheniformis</i> induced the best rates in stem growth. These findings highlight the potential for using endophytic bacterial strains in a hydroponic co-cultivation system that enables inoculating plantlets, at an early stage of growth (5 days old).https://www.mdpi.com/2673-6284/13/3/32endophytic bacteria<i>Azospirillum</i> spp.<i>Bacillus</i> spp.hydroponicIAArhizobacteria |
| spellingShingle | Livia Pappalettere Susanna Bartolini Annita Toffanin Auxin-Producing Bacteria Used as Microbial Biostimulants Improve the Growth of Tomato (<i>Solanum lycopersicum</i> L.) Seedlings in Hydroponic Systems BioTech endophytic bacteria <i>Azospirillum</i> spp. <i>Bacillus</i> spp. hydroponic IAA rhizobacteria |
| title | Auxin-Producing Bacteria Used as Microbial Biostimulants Improve the Growth of Tomato (<i>Solanum lycopersicum</i> L.) Seedlings in Hydroponic Systems |
| title_full | Auxin-Producing Bacteria Used as Microbial Biostimulants Improve the Growth of Tomato (<i>Solanum lycopersicum</i> L.) Seedlings in Hydroponic Systems |
| title_fullStr | Auxin-Producing Bacteria Used as Microbial Biostimulants Improve the Growth of Tomato (<i>Solanum lycopersicum</i> L.) Seedlings in Hydroponic Systems |
| title_full_unstemmed | Auxin-Producing Bacteria Used as Microbial Biostimulants Improve the Growth of Tomato (<i>Solanum lycopersicum</i> L.) Seedlings in Hydroponic Systems |
| title_short | Auxin-Producing Bacteria Used as Microbial Biostimulants Improve the Growth of Tomato (<i>Solanum lycopersicum</i> L.) Seedlings in Hydroponic Systems |
| title_sort | auxin producing bacteria used as microbial biostimulants improve the growth of tomato i solanum lycopersicum i l seedlings in hydroponic systems |
| topic | endophytic bacteria <i>Azospirillum</i> spp. <i>Bacillus</i> spp. hydroponic IAA rhizobacteria |
| url | https://www.mdpi.com/2673-6284/13/3/32 |
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