Study of the Chemical Composition and Anti-Inflammatory Mechanism of Shiyiwei Golden Pill Based on UPLC-Q-TOF/MS and Network Pharmacology
Cong Han,1,* Jing Chen,2,* Chuanlin Shen,1 Qiuxia Liang,1 Ying An,1 Chaoyi Zhou,1 Kechun Liu,1 Qing Xia,1 Qiuxia He,1 Huazheng Zhang3 1Biology Institute, Qilu University of Technology (Shandong Academy of Sciences), Jinan, Shandong, People’s Republic of China; 2Department of Tibetan...
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Dove Medical Press
2025-04-01
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| author | Han C Chen J Shen C Liang Q An Y Zhou C Liu K Xia Q He Q Zhang H |
| author_facet | Han C Chen J Shen C Liang Q An Y Zhou C Liu K Xia Q He Q Zhang H |
| author_sort | Han C |
| collection | DOAJ |
| description | Cong Han,1,* Jing Chen,2,* Chuanlin Shen,1 Qiuxia Liang,1 Ying An,1 Chaoyi Zhou,1 Kechun Liu,1 Qing Xia,1 Qiuxia He,1 Huazheng Zhang3 1Biology Institute, Qilu University of Technology (Shandong Academy of Sciences), Jinan, Shandong, People’s Republic of China; 2Department of Tibetan Medicine, Tibetan Traditional Medicine College, Lhasa, Tibet, People’s Republic of China; 3Shandong Academy of Chinese Medicine, Jinan, Shandong, People’s Republic of China*These authors contributed equally to this workCorrespondence: Qing Xia, Biology Institute, Qilu University of Technology (Shandong Academy of Sciences), Jinan, 250103, People’s Republic of China, Email sdxq1021@163.com Huazheng Zhang, Shandong Academy of Chinese Medicine, Jinan, 250014, People’s Republic of China, Email zhanghuazheng1109@163.comPurpose: Shiyiwei Golden Pill (SYW) is a classic traditional prescription used to treat mKhris-pa according to the theory of Tibetan medicine. At present, SYW is widely used to treat cholecystitis in Tibetan areas. However, the chemical constituents and anti-inflammatory mechanisms are still largely undiscovered. This study aimed to investigate the chemical composition and anti-inflammatory effects of SYW, as well as its potential mechanisms.Methods: The components of SYW were identified using ultrahigh-performance liquid chromatography with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS). The anti-inflammatory effects of SYW were determined on zebrafish and RAW264.7 cell inflammation models. Additionally, we predicted the targets and pathways of SYW to confirm its anti-inflammatory effects using network pharmacology approaches. Finally, a quantitative real-time polymerase chain reaction (qRT–PCR) was performed to validate the expression of genes associated with anti-inflammatory pathways.Results: We identified 94 compounds in SYW, mainly alkaloids, phenols, and flavonoids. SYW inhibited inflammatory cell proliferation and migration in the three zebrafish inflammation models. In the RAW264.7 cell model, SYW suppressed the levels of NO and pro-inflammatory cytokines. In addition, network pharmacology analysis revealed that ALB, IL6, TNF, AKT1, and EGFR were identified as the potential key targets of SYW. KEGG enrichment and qRT–PCR analysis showed that PI3K/Akt/FoxO signaling pathway was involved in the anti-inflammatory effects of SYW.Conclusion: Herein, we identified 94 chemical constituents of SYW and demonstrated that SYW exerts anti-inflammatory effects by regulating the PI3K/Akt/FoxO signaling pathway.Keywords: shiyiwei golden pill, inflammation, zebrafish, network pharmacology, PI3K/Akt/FoxO signaling pathway, cholecystitis |
| format | Article |
| id | doaj-art-1d7bbad2cd1d441bb5aa6b405d823367 |
| institution | Kabale University |
| issn | 1177-8881 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Dove Medical Press |
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| series | Drug Design, Development and Therapy |
| spelling | doaj-art-1d7bbad2cd1d441bb5aa6b405d8233672025-08-20T03:53:32ZengDove Medical PressDrug Design, Development and Therapy1177-88812025-04-01Volume 1931593177102357Study of the Chemical Composition and Anti-Inflammatory Mechanism of Shiyiwei Golden Pill Based on UPLC-Q-TOF/MS and Network PharmacologyHan CChen JShen CLiang QAn YZhou CLiu KXia QHe QZhang HCong Han,1,* Jing Chen,2,* Chuanlin Shen,1 Qiuxia Liang,1 Ying An,1 Chaoyi Zhou,1 Kechun Liu,1 Qing Xia,1 Qiuxia He,1 Huazheng Zhang3 1Biology Institute, Qilu University of Technology (Shandong Academy of Sciences), Jinan, Shandong, People’s Republic of China; 2Department of Tibetan Medicine, Tibetan Traditional Medicine College, Lhasa, Tibet, People’s Republic of China; 3Shandong Academy of Chinese Medicine, Jinan, Shandong, People’s Republic of China*These authors contributed equally to this workCorrespondence: Qing Xia, Biology Institute, Qilu University of Technology (Shandong Academy of Sciences), Jinan, 250103, People’s Republic of China, Email sdxq1021@163.com Huazheng Zhang, Shandong Academy of Chinese Medicine, Jinan, 250014, People’s Republic of China, Email zhanghuazheng1109@163.comPurpose: Shiyiwei Golden Pill (SYW) is a classic traditional prescription used to treat mKhris-pa according to the theory of Tibetan medicine. At present, SYW is widely used to treat cholecystitis in Tibetan areas. However, the chemical constituents and anti-inflammatory mechanisms are still largely undiscovered. This study aimed to investigate the chemical composition and anti-inflammatory effects of SYW, as well as its potential mechanisms.Methods: The components of SYW were identified using ultrahigh-performance liquid chromatography with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS). The anti-inflammatory effects of SYW were determined on zebrafish and RAW264.7 cell inflammation models. Additionally, we predicted the targets and pathways of SYW to confirm its anti-inflammatory effects using network pharmacology approaches. Finally, a quantitative real-time polymerase chain reaction (qRT–PCR) was performed to validate the expression of genes associated with anti-inflammatory pathways.Results: We identified 94 compounds in SYW, mainly alkaloids, phenols, and flavonoids. SYW inhibited inflammatory cell proliferation and migration in the three zebrafish inflammation models. In the RAW264.7 cell model, SYW suppressed the levels of NO and pro-inflammatory cytokines. In addition, network pharmacology analysis revealed that ALB, IL6, TNF, AKT1, and EGFR were identified as the potential key targets of SYW. KEGG enrichment and qRT–PCR analysis showed that PI3K/Akt/FoxO signaling pathway was involved in the anti-inflammatory effects of SYW.Conclusion: Herein, we identified 94 chemical constituents of SYW and demonstrated that SYW exerts anti-inflammatory effects by regulating the PI3K/Akt/FoxO signaling pathway.Keywords: shiyiwei golden pill, inflammation, zebrafish, network pharmacology, PI3K/Akt/FoxO signaling pathway, cholecystitishttps://www.dovepress.com/study-of-the-chemical-composition-and-anti-inflammatory-mechanism-of-s-peer-reviewed-fulltext-article-DDDTshiyiwei golden pillinflammationzebrafishnetwork pharmacologypi3k/akt/foxo signaling pathwaycholecystitis |
| spellingShingle | Han C Chen J Shen C Liang Q An Y Zhou C Liu K Xia Q He Q Zhang H Study of the Chemical Composition and Anti-Inflammatory Mechanism of Shiyiwei Golden Pill Based on UPLC-Q-TOF/MS and Network Pharmacology Drug Design, Development and Therapy shiyiwei golden pill inflammation zebrafish network pharmacology pi3k/akt/foxo signaling pathway cholecystitis |
| title | Study of the Chemical Composition and Anti-Inflammatory Mechanism of Shiyiwei Golden Pill Based on UPLC-Q-TOF/MS and Network Pharmacology |
| title_full | Study of the Chemical Composition and Anti-Inflammatory Mechanism of Shiyiwei Golden Pill Based on UPLC-Q-TOF/MS and Network Pharmacology |
| title_fullStr | Study of the Chemical Composition and Anti-Inflammatory Mechanism of Shiyiwei Golden Pill Based on UPLC-Q-TOF/MS and Network Pharmacology |
| title_full_unstemmed | Study of the Chemical Composition and Anti-Inflammatory Mechanism of Shiyiwei Golden Pill Based on UPLC-Q-TOF/MS and Network Pharmacology |
| title_short | Study of the Chemical Composition and Anti-Inflammatory Mechanism of Shiyiwei Golden Pill Based on UPLC-Q-TOF/MS and Network Pharmacology |
| title_sort | study of the chemical composition and anti inflammatory mechanism of shiyiwei golden pill based on uplc q tof ms and network pharmacology |
| topic | shiyiwei golden pill inflammation zebrafish network pharmacology pi3k/akt/foxo signaling pathway cholecystitis |
| url | https://www.dovepress.com/study-of-the-chemical-composition-and-anti-inflammatory-mechanism-of-s-peer-reviewed-fulltext-article-DDDT |
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