High uric acid model in Caenorhabditis elegans
To establish experimental high uric acid model in C. elegans. Hypoxanthine, adenine, xanthine, and uric acid were used to treat C. elegans and then hyperuricemic C. elegans was evaluated by allopurinol. Hyperuricemic C. elegans were obtained after normal worms were treated by xanthine (0.25 mg/mL, 1...
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| Format: | Article |
| Language: | English |
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Tsinghua University Press
2019-03-01
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| Series: | Food Science and Human Wellness |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213453018301381 |
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| author | Zhenjing Li Yibin Xue Nifei Wang Jingli Cheng Xiaoying Dong Qingbin Guo Changlu Wang |
| author_facet | Zhenjing Li Yibin Xue Nifei Wang Jingli Cheng Xiaoying Dong Qingbin Guo Changlu Wang |
| author_sort | Zhenjing Li |
| collection | DOAJ |
| description | To establish experimental high uric acid model in C. elegans. Hypoxanthine, adenine, xanthine, and uric acid were used to treat C. elegans and then hyperuricemic C. elegans was evaluated by allopurinol. Hyperuricemic C. elegans were obtained after normal worms were treated by xanthine (0.25 mg/mL, 18 h). For hyperuricemic worms, there was a statistically significant increase in the uric acid level (p < 0.001) and a lower drug damage (p > 0.05). Moreover, the model was proved to keep a high uric acid level for up to 12 h. After given allopurinol (0.25 mg/mL, 12 h), the uric acid of hyperuricemic C. elegans had a significant reduction by 15%. Furthermore, xanthine oxidase activity in hyperuricemic C. elegans showed a statistically significant increase (p < 0.001), which resulted in a raised uric acid content. A high uric acid model with low drug damage and high efficiency and stability was established in C. elegans after simply xanthine treatment. Keywords: Hyperuricemia, C. elegans, Uric acid, Xanthine, Xanthine oxidase |
| format | Article |
| id | doaj-art-1d794c3fc075401285ee776ae17139b9 |
| institution | Kabale University |
| issn | 2213-4530 |
| language | English |
| publishDate | 2019-03-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-1d794c3fc075401285ee776ae17139b92025-02-03T06:55:44ZengTsinghua University PressFood Science and Human Wellness2213-45302019-03-01816366High uric acid model in Caenorhabditis elegansZhenjing Li0Yibin Xue1Nifei Wang2Jingli Cheng3Xiaoying Dong4Qingbin Guo5Changlu Wang6State Key Laboratory of Food Nutrition and Safety, Tianjin University of Science & Technology, Tianjin 300457, China; Key Laboratory of Food Nutrition and Safety, Ministry of Education, School of Food Engineering and Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, ChinaState Key Laboratory of Food Nutrition and Safety, Tianjin University of Science & Technology, Tianjin 300457, China; Key Laboratory of Food Nutrition and Safety, Ministry of Education, School of Food Engineering and Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, ChinaState Key Laboratory of Food Nutrition and Safety, Tianjin University of Science & Technology, Tianjin 300457, China; Key Laboratory of Food Nutrition and Safety, Ministry of Education, School of Food Engineering and Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, ChinaDepartment of nephrology, Metabolic Disease Hospital, Tianjin Medical University, Key Laboratory of Hormones and Development, Ministry of Health, Tianjin 300070, ChinaState Key Laboratory of Food Nutrition and Safety, Tianjin University of Science & Technology, Tianjin 300457, China; Key Laboratory of Food Nutrition and Safety, Ministry of Education, School of Food Engineering and Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, ChinaState Key Laboratory of Food Nutrition and Safety, Tianjin University of Science & Technology, Tianjin 300457, China; Key Laboratory of Food Nutrition and Safety, Ministry of Education, School of Food Engineering and Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, ChinaState Key Laboratory of Food Nutrition and Safety, Tianjin University of Science & Technology, Tianjin 300457, China; Key Laboratory of Food Nutrition and Safety, Ministry of Education, School of Food Engineering and Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China; Corresponding author at: Tianjin University of Science & Technology, Economic and Technological Development Zone, 13th Avenue No. 29, Tianjin 300457, China.To establish experimental high uric acid model in C. elegans. Hypoxanthine, adenine, xanthine, and uric acid were used to treat C. elegans and then hyperuricemic C. elegans was evaluated by allopurinol. Hyperuricemic C. elegans were obtained after normal worms were treated by xanthine (0.25 mg/mL, 18 h). For hyperuricemic worms, there was a statistically significant increase in the uric acid level (p < 0.001) and a lower drug damage (p > 0.05). Moreover, the model was proved to keep a high uric acid level for up to 12 h. After given allopurinol (0.25 mg/mL, 12 h), the uric acid of hyperuricemic C. elegans had a significant reduction by 15%. Furthermore, xanthine oxidase activity in hyperuricemic C. elegans showed a statistically significant increase (p < 0.001), which resulted in a raised uric acid content. A high uric acid model with low drug damage and high efficiency and stability was established in C. elegans after simply xanthine treatment. Keywords: Hyperuricemia, C. elegans, Uric acid, Xanthine, Xanthine oxidasehttp://www.sciencedirect.com/science/article/pii/S2213453018301381 |
| spellingShingle | Zhenjing Li Yibin Xue Nifei Wang Jingli Cheng Xiaoying Dong Qingbin Guo Changlu Wang High uric acid model in Caenorhabditis elegans Food Science and Human Wellness |
| title | High uric acid model in Caenorhabditis elegans |
| title_full | High uric acid model in Caenorhabditis elegans |
| title_fullStr | High uric acid model in Caenorhabditis elegans |
| title_full_unstemmed | High uric acid model in Caenorhabditis elegans |
| title_short | High uric acid model in Caenorhabditis elegans |
| title_sort | high uric acid model in caenorhabditis elegans |
| url | http://www.sciencedirect.com/science/article/pii/S2213453018301381 |
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