Occurrence and characteristics of extended-spectrum ß-lactamase-producing Escherichia coli in Swiss and imported retail chicken meat
Objective: The occurrence of extended-spectrum ß-lactamase (ESBL) producing Enterobacterales in food is of concern because of the possibility of transmission of ESBL-producers and/or blaESBL genes to humans. This study aimed to investigate the presence of ESBL-producing Enterobacterales in Swiss and...
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Elsevier
2025-06-01
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| Series: | Journal of Global Antimicrobial Resistance |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213716525001158 |
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| author | Lucien Kelbert Karen Barmettler Michael Biggel Roger Stephan Magdalena Nüesch-Inderbinen |
| author_facet | Lucien Kelbert Karen Barmettler Michael Biggel Roger Stephan Magdalena Nüesch-Inderbinen |
| author_sort | Lucien Kelbert |
| collection | DOAJ |
| description | Objective: The occurrence of extended-spectrum ß-lactamase (ESBL) producing Enterobacterales in food is of concern because of the possibility of transmission of ESBL-producers and/or blaESBL genes to humans. This study aimed to investigate the presence of ESBL-producing Enterobacterales in Swiss and imported retail chicken meat. Methods: A total of 200 samples of different types of Swiss and imported chicken meat obtained at retail level in Switzerland were screened for ESBL-producing Enterobacterales using a selective culture medium. All non-intrinsically resistant isolates were characterised by antimicrobial susceptibility testing (AST), multilocus sequence typing (MLST), and whole genome sequencing (WGS). Results: Eighteen meat samples (9%) yielded a total of 19 ESBL-producing Escherichia coli (ESBL-EC). The proportion of samples containing ESBL-EC was higher in imported meat (14/55; 25%) than in Swiss meat (4/145; 3%). Co-resistance to ciprofloxacin was highly prevalent (18/19, 95%). E. coli sequence types (STs) included ST602, ST744, ST1844, and extraintestinal pathogenic (ExPEC) E. coli ST38. The blaESBL genes comprised blaCTX-M-1 (n=7), blaCTX-M-2 (n=1), blaCTX-M-8 (n=1), blaCTX-M-55 (n=1), blaSHV-12 (n=8), and blaTEM-52B (n=1). They were chromosomally (n=2) encoded or carried on plasmids belonging to IncB/O/K/Z (n=1), IncFII (n=1), IncI1-I(Alpha) (n=9), IncX1 (n=1), and IncX3 (n=5). Many of the plasmids were identical to those detected globally in ESBL-producers associated with broilers. Conclusions: The contamination with ESBL-EC in retail chicken meat in Switzerland is moderate; nevertheless, domestic and imported chicken meat is a potential vehicle for MDR ESBL-EC and for genes conferring resistance to clinically important antimicrobials including 3rd-generation cephalosporins and fluoroquinolones. |
| format | Article |
| id | doaj-art-1d5e667ccfaa4dcbba7101c8be6adab0 |
| institution | Kabale University |
| issn | 2213-7165 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
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| series | Journal of Global Antimicrobial Resistance |
| spelling | doaj-art-1d5e667ccfaa4dcbba7101c8be6adab02025-08-20T03:32:02ZengElsevierJournal of Global Antimicrobial Resistance2213-71652025-06-014328529210.1016/j.jgar.2025.05.013Occurrence and characteristics of extended-spectrum ß-lactamase-producing Escherichia coli in Swiss and imported retail chicken meatLucien Kelbert0Karen Barmettler1Michael Biggel2Roger Stephan3Magdalena Nüesch-Inderbinen4Institute for Food Safety and Hygiene, Vetsuisse Faculty, University of Zurich, Zurich, SwitzerlandInstitute for Food Safety and Hygiene, Vetsuisse Faculty, University of Zurich, Zurich, SwitzerlandInstitute for Food Safety and Hygiene, Vetsuisse Faculty, University of Zurich, Zurich, SwitzerlandInstitute for Food Safety and Hygiene, Vetsuisse Faculty, University of Zurich, Zurich, SwitzerlandCorresponding author. Mailing address: Magdalena Nüesch-Inderbinen, Institute for Food Safety and Hygiene, Vetsuisse Faculty, University of Zurich, Winterthurerstrasse 272, CH-8057 Zurich, Switzerland.; Institute for Food Safety and Hygiene, Vetsuisse Faculty, University of Zurich, Zurich, SwitzerlandObjective: The occurrence of extended-spectrum ß-lactamase (ESBL) producing Enterobacterales in food is of concern because of the possibility of transmission of ESBL-producers and/or blaESBL genes to humans. This study aimed to investigate the presence of ESBL-producing Enterobacterales in Swiss and imported retail chicken meat. Methods: A total of 200 samples of different types of Swiss and imported chicken meat obtained at retail level in Switzerland were screened for ESBL-producing Enterobacterales using a selective culture medium. All non-intrinsically resistant isolates were characterised by antimicrobial susceptibility testing (AST), multilocus sequence typing (MLST), and whole genome sequencing (WGS). Results: Eighteen meat samples (9%) yielded a total of 19 ESBL-producing Escherichia coli (ESBL-EC). The proportion of samples containing ESBL-EC was higher in imported meat (14/55; 25%) than in Swiss meat (4/145; 3%). Co-resistance to ciprofloxacin was highly prevalent (18/19, 95%). E. coli sequence types (STs) included ST602, ST744, ST1844, and extraintestinal pathogenic (ExPEC) E. coli ST38. The blaESBL genes comprised blaCTX-M-1 (n=7), blaCTX-M-2 (n=1), blaCTX-M-8 (n=1), blaCTX-M-55 (n=1), blaSHV-12 (n=8), and blaTEM-52B (n=1). They were chromosomally (n=2) encoded or carried on plasmids belonging to IncB/O/K/Z (n=1), IncFII (n=1), IncI1-I(Alpha) (n=9), IncX1 (n=1), and IncX3 (n=5). Many of the plasmids were identical to those detected globally in ESBL-producers associated with broilers. Conclusions: The contamination with ESBL-EC in retail chicken meat in Switzerland is moderate; nevertheless, domestic and imported chicken meat is a potential vehicle for MDR ESBL-EC and for genes conferring resistance to clinically important antimicrobials including 3rd-generation cephalosporins and fluoroquinolones.http://www.sciencedirect.com/science/article/pii/S2213716525001158Chicken meatEscherichia coliCTX-M-1SHV-12IncI1-I(Alpha)IncX3 |
| spellingShingle | Lucien Kelbert Karen Barmettler Michael Biggel Roger Stephan Magdalena Nüesch-Inderbinen Occurrence and characteristics of extended-spectrum ß-lactamase-producing Escherichia coli in Swiss and imported retail chicken meat Journal of Global Antimicrobial Resistance Chicken meat Escherichia coli CTX-M-1 SHV-12 IncI1-I(Alpha) IncX3 |
| title | Occurrence and characteristics of extended-spectrum ß-lactamase-producing Escherichia coli in Swiss and imported retail chicken meat |
| title_full | Occurrence and characteristics of extended-spectrum ß-lactamase-producing Escherichia coli in Swiss and imported retail chicken meat |
| title_fullStr | Occurrence and characteristics of extended-spectrum ß-lactamase-producing Escherichia coli in Swiss and imported retail chicken meat |
| title_full_unstemmed | Occurrence and characteristics of extended-spectrum ß-lactamase-producing Escherichia coli in Swiss and imported retail chicken meat |
| title_short | Occurrence and characteristics of extended-spectrum ß-lactamase-producing Escherichia coli in Swiss and imported retail chicken meat |
| title_sort | occurrence and characteristics of extended spectrum ss lactamase producing escherichia coli in swiss and imported retail chicken meat |
| topic | Chicken meat Escherichia coli CTX-M-1 SHV-12 IncI1-I(Alpha) IncX3 |
| url | http://www.sciencedirect.com/science/article/pii/S2213716525001158 |
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