Estimation of MRSA Susceptibility to Oxacillin, Cefoxitine, Vancomycin and Daptomyci
Prevalence and therapy of infections due to MRSA remain one of the most serious problems in the world. Therefore, correct laboratory identification of the MRSA phenotype based on the use of the marker antibiotic cefoxitine, as a more susceptibile one vs. oxacillin, is of great importance. There is l...
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2020-05-01
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| Series: | Антибиотики и Химиотерапия |
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| Online Access: | https://www.antibiotics-chemotherapy.ru/jour/article/view/505 |
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| author | V. V. Gostev L. N. Popenko T. V. Chernenkaya Z. S. Naumenko T. M. Voroshilova Yu. A. Zakharova O. E. Khokhlova A. N. Kruglov M. G. Ershova S. N. Angelova E. D. Poletaeva I. V. Molchanova S. V. Sidorenko |
| author_facet | V. V. Gostev L. N. Popenko T. V. Chernenkaya Z. S. Naumenko T. M. Voroshilova Yu. A. Zakharova O. E. Khokhlova A. N. Kruglov M. G. Ershova S. N. Angelova E. D. Poletaeva I. V. Molchanova S. V. Sidorenko |
| author_sort | V. V. Gostev |
| collection | DOAJ |
| description | Prevalence and therapy of infections due to MRSA remain one of the most serious problems in the world. Therefore, correct laboratory identification of the MRSA phenotype based on the use of the marker antibiotic cefoxitine, as a more susceptibile one vs. oxacillin, is of great importance. There is lately being observed a tendency towards emergence of strains with lower susceptibility to the last reserve drugs protecting from MRSA, i. e. vancomycin amd daptomycin. Susceptibility of MSRA to these drugs was not investigated in Russia and there are no data on the prevalence of the VISA and hVISA phenotypes. The results of our study on estimation of susceptibility of 316 MRSA isolates from several regions of Russia to oxacillin, cefoxitine, vancomycin and daptomycin are presented herein. It was shown that the ranges of the oxacillin MIC were extremely wide, i. e. 0.5 to 512 mcg/ml, while 2.2±1% of the isolates was susceptible by the phenotype to oxacillin, in spite of the mecA gene presence. As for cefoxitine, the MRSA isolates were rather resistant to it at the MIC > 16 mcg/ml. The tests with serial microdilutions revealed that 30.7±7% of the isolates had a critical level of susceptibility to vancomycin at the MIC 2 mcg/ml. The E-tests revealed 1.3±1% of the isolates which were susceptible at the MIC 2-4 mcg/ml. The MRSA isolates were highly susceptible to daptomycin, while high levels of the MIC (2 mcg/ml) were characteristic of 2.8±1% of the isolates. Cross reduction of the susceptibility to vancomycin and daptomycin was observed. |
| format | Article |
| id | doaj-art-1d42577f8e424edda35d2a63424c4487 |
| institution | Kabale University |
| issn | 0235-2990 |
| language | Russian |
| publishDate | 2020-05-01 |
| publisher | LLC "Publishing House OKI" |
| record_format | Article |
| series | Антибиотики и Химиотерапия |
| spelling | doaj-art-1d42577f8e424edda35d2a63424c44872025-08-20T03:42:01ZrusLLC "Publishing House OKI"Антибиотики и Химиотерапия0235-29902020-05-01589-101320504Estimation of MRSA Susceptibility to Oxacillin, Cefoxitine, Vancomycin and DaptomyciV. V. Gostev0L. N. Popenko1T. V. Chernenkaya2Z. S. Naumenko3T. M. Voroshilova4Yu. A. Zakharova5O. E. Khokhlova6A. N. Kruglov7M. G. Ershova8S. N. Angelova9E. D. Poletaeva10I. V. Molchanova11S. V. Sidorenko12Research Institute of Children's InfectionsI.I. Dzhanelidze Research Institute of Emergency ServiceN.V. Sklifosovsky Research Institute of Emergency ServiceG.A. Ilizarov Russian Research Centre of Restorative Traumatology and OrthopedicsA.M. Nikiforov Russian Center of Emergency and Radiation MedicinePerm Clinical CentreV.F. Voino-Yasenetski Krasnoyarsk State Medical UniversityNational Agency of Clinical Pharmacology and PharmacyInfectious Clinical Hospital No. 1Infectious Clinical Hospital No. 1Infectious Clinical Hospital No. 1Chelyabinsk Regional Clinical HospitalResearch Institute of Children's InfectionsPrevalence and therapy of infections due to MRSA remain one of the most serious problems in the world. Therefore, correct laboratory identification of the MRSA phenotype based on the use of the marker antibiotic cefoxitine, as a more susceptibile one vs. oxacillin, is of great importance. There is lately being observed a tendency towards emergence of strains with lower susceptibility to the last reserve drugs protecting from MRSA, i. e. vancomycin amd daptomycin. Susceptibility of MSRA to these drugs was not investigated in Russia and there are no data on the prevalence of the VISA and hVISA phenotypes. The results of our study on estimation of susceptibility of 316 MRSA isolates from several regions of Russia to oxacillin, cefoxitine, vancomycin and daptomycin are presented herein. It was shown that the ranges of the oxacillin MIC were extremely wide, i. e. 0.5 to 512 mcg/ml, while 2.2±1% of the isolates was susceptible by the phenotype to oxacillin, in spite of the mecA gene presence. As for cefoxitine, the MRSA isolates were rather resistant to it at the MIC > 16 mcg/ml. The tests with serial microdilutions revealed that 30.7±7% of the isolates had a critical level of susceptibility to vancomycin at the MIC 2 mcg/ml. The E-tests revealed 1.3±1% of the isolates which were susceptible at the MIC 2-4 mcg/ml. The MRSA isolates were highly susceptible to daptomycin, while high levels of the MIC (2 mcg/ml) were characteristic of 2.8±1% of the isolates. Cross reduction of the susceptibility to vancomycin and daptomycin was observed.https://www.antibiotics-chemotherapy.ru/jour/article/view/505methicillin resistant staphylococci (mrsa)oxacillincefoxitinevancomycindaptomycin |
| spellingShingle | V. V. Gostev L. N. Popenko T. V. Chernenkaya Z. S. Naumenko T. M. Voroshilova Yu. A. Zakharova O. E. Khokhlova A. N. Kruglov M. G. Ershova S. N. Angelova E. D. Poletaeva I. V. Molchanova S. V. Sidorenko Estimation of MRSA Susceptibility to Oxacillin, Cefoxitine, Vancomycin and Daptomyci Антибиотики и Химиотерапия methicillin resistant staphylococci (mrsa) oxacillin cefoxitine vancomycin daptomycin |
| title | Estimation of MRSA Susceptibility to Oxacillin, Cefoxitine, Vancomycin and Daptomyci |
| title_full | Estimation of MRSA Susceptibility to Oxacillin, Cefoxitine, Vancomycin and Daptomyci |
| title_fullStr | Estimation of MRSA Susceptibility to Oxacillin, Cefoxitine, Vancomycin and Daptomyci |
| title_full_unstemmed | Estimation of MRSA Susceptibility to Oxacillin, Cefoxitine, Vancomycin and Daptomyci |
| title_short | Estimation of MRSA Susceptibility to Oxacillin, Cefoxitine, Vancomycin and Daptomyci |
| title_sort | estimation of mrsa susceptibility to oxacillin cefoxitine vancomycin and daptomyci |
| topic | methicillin resistant staphylococci (mrsa) oxacillin cefoxitine vancomycin daptomycin |
| url | https://www.antibiotics-chemotherapy.ru/jour/article/view/505 |
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