Effect of high glucose on expression of adiponectin through ERK1/2 signaling pathway in HK-2 cells
Objective Adiponectin is closely related to the development of diabetic kidney disease.Recent evidence suggests that human proximal tubular epithelial cells could express and secrete adiponectin and the expression of adiponectin could be inhibited by high glucose.However,the precise mechanisms remai...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | zho |
| Published: |
Editorial Department of Journal of Clinical Nephrology
2016-01-01
|
| Series: | Linchuang shenzangbing zazhi |
| Subjects: | |
| Online Access: | http://www.lcszb.com/thesisDetails?columnId=57918787&Fpath=home&index=0 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | Objective Adiponectin is closely related to the development of diabetic kidney disease.Recent evidence suggests that human proximal tubular epithelial cells could express and secrete adiponectin and the expression of adiponectin could be inhibited by high glucose.However,the precise mechanisms remain poorly understood.We aim to observe the effect of high glucose on the expression of adiponectin and to explore its possible mechanism in human proximal tubular epithelial cells in vitro.Methods Human proximal tubular epithelial cells were cultivated in four groups for 48 h:normal glucose group(NG,5.6 mmol/L D-glucose);PD98059 group(PD,5.6 mmol/L D-glucose with 50μmol/L PD98059);high glucose group(HG,30 mmol/L D-glucose);high glucose with PD98059group(HG+PD,30 mmol/L D-glucose with 50 μmol/L PD98059).The mRNA expression of peroxisome proliferator activator receptor(PPAR)-γ and adiponectin was detected by real-time polymerase chain reaction(PCR)and the protein level of adiponectin by Western blotting.Results HK-2 cells expressed adiponectin.As compared with normal glucose group,There was no significant difference in the expression level of PPAR-γ and adiponectin mRNA and adiponectin protein between normal glucose group and PD98059 group(P>0.05),but the expression level of PPAR-γ and adiponectin mRNA and adiponectin protein decreased significantly in high glucose group as compared with normal glucose group(P<0.05).As compared with high glucose group,the expression of PPAR-γ and adiponectin mRNA and adiponectin protein increased significantly in high glucose with PD98059 group(P<0.05).Conclusions HK-2 cells expressed adiponectin.The high glucose may reduce the expression of PPAR-γ mRNA through the ERK1/2 signaling pathway,thus reducing the expression of adiponectin in HK-2 cells. |
|---|---|
| ISSN: | 1671-2390 |