Features of immune status in COVID-19 convalescents
Study objective — assessment of the humoral and cell-mediated immunity features in COVID-19 convalescents three months after their discharge from the hospital. Material and Methods — The study involved 78 COVID-19 convalescents who, depending on the profile of specific IgM and IgG antibodies to SAR...
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| Format: | Article |
| Language: | English |
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Limited liability company «Science and Innovations» (Saratov)
2022-06-01
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| Series: | Russian Open Medical Journal |
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| Online Access: | https://romj.org/node/468 |
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| author | Alina S. Shkaberda Elena A. Kordovskaya Diana Yu. Bakuta Lyubov I. Plotnichenko Alexander A. Gorbunov Leya E. Sorokina |
| author_facet | Alina S. Shkaberda Elena A. Kordovskaya Diana Yu. Bakuta Lyubov I. Plotnichenko Alexander A. Gorbunov Leya E. Sorokina |
| author_sort | Alina S. Shkaberda |
| collection | DOAJ |
| description | Study objective — assessment of the humoral and cell-mediated immunity features in COVID-19 convalescents three months after their discharge from the hospital.
Material and Methods — The study involved 78 COVID-19 convalescents who, depending on the profile of specific IgM and IgG antibodies to SARS-CoV-2, were divided into three groups. The control group consisted of 50 volunteers. Detection of IgM and IgG in blood serum was performed by ELISA. Determination of CRP concentration was conducted using the immunoturbidimetric assay. To determine the levels of IL-6, a sandwich version of the solid-phase ELISA was employed. Immunophenotyping of lymphocytes was performed via flow cytometry.
Results — Of 78 COVID-19 convalescents three months after their discharge from the hospital, 30.8% of them had a profile of specific antibodies IgM(+)IgG(+), 37.2% had IgM(-)IgG(+), and 32.0% were characterized by IgM(-)IgG (-). COVID-19 convalescents with an IgM(-)IgG(-) profile had the highest levels of NK cells, T helper cells, B lymphocytes (p<0.001) and were characterized by hyperproduction of proinflammatory IL-6 (p<0.001). COVID-19 convalescents with an IgM(+)IgG(+) specific antibody profile were characterized by the highest levels of cytotoxic T lymphocytes (p<0.001). In a COVID-19 convalescent with an IgM(-)IgG(+) specific antibody profile, we observed an increase in the number of lymphocytes expressing late activation/apoptosis molecules (p<0.001).
Conclusion — The collected data is of potential importance in clinical practice for developing a prognosis for epidemiological situation development, as well as for planning preventive measures to COVID-19. |
| format | Article |
| id | doaj-art-1d2f6c96fe4c490883e31d1f4fc38782 |
| institution | OA Journals |
| issn | 2304-3415 |
| language | English |
| publishDate | 2022-06-01 |
| publisher | Limited liability company «Science and Innovations» (Saratov) |
| record_format | Article |
| series | Russian Open Medical Journal |
| spelling | doaj-art-1d2f6c96fe4c490883e31d1f4fc387822025-08-20T02:03:05ZengLimited liability company «Science and Innovations» (Saratov)Russian Open Medical Journal2304-34152022-06-01112e020610.15275/rusomj.2022.0206Features of immune status in COVID-19 convalescentsAlina S. ShkaberdaElena A. KordovskayaDiana Yu. BakutaLyubov I. PlotnichenkoAlexander A. GorbunovLeya E. SorokinaStudy objective — assessment of the humoral and cell-mediated immunity features in COVID-19 convalescents three months after their discharge from the hospital. Material and Methods — The study involved 78 COVID-19 convalescents who, depending on the profile of specific IgM and IgG antibodies to SARS-CoV-2, were divided into three groups. The control group consisted of 50 volunteers. Detection of IgM and IgG in blood serum was performed by ELISA. Determination of CRP concentration was conducted using the immunoturbidimetric assay. To determine the levels of IL-6, a sandwich version of the solid-phase ELISA was employed. Immunophenotyping of lymphocytes was performed via flow cytometry. Results — Of 78 COVID-19 convalescents three months after their discharge from the hospital, 30.8% of them had a profile of specific antibodies IgM(+)IgG(+), 37.2% had IgM(-)IgG(+), and 32.0% were characterized by IgM(-)IgG (-). COVID-19 convalescents with an IgM(-)IgG(-) profile had the highest levels of NK cells, T helper cells, B lymphocytes (p<0.001) and were characterized by hyperproduction of proinflammatory IL-6 (p<0.001). COVID-19 convalescents with an IgM(+)IgG(+) specific antibody profile were characterized by the highest levels of cytotoxic T lymphocytes (p<0.001). In a COVID-19 convalescent with an IgM(-)IgG(+) specific antibody profile, we observed an increase in the number of lymphocytes expressing late activation/apoptosis molecules (p<0.001). Conclusion — The collected data is of potential importance in clinical practice for developing a prognosis for epidemiological situation development, as well as for planning preventive measures to COVID-19.https://romj.org/node/468covid-19specific antibodiesimmune status |
| spellingShingle | Alina S. Shkaberda Elena A. Kordovskaya Diana Yu. Bakuta Lyubov I. Plotnichenko Alexander A. Gorbunov Leya E. Sorokina Features of immune status in COVID-19 convalescents Russian Open Medical Journal covid-19 specific antibodies immune status |
| title | Features of immune status in COVID-19 convalescents |
| title_full | Features of immune status in COVID-19 convalescents |
| title_fullStr | Features of immune status in COVID-19 convalescents |
| title_full_unstemmed | Features of immune status in COVID-19 convalescents |
| title_short | Features of immune status in COVID-19 convalescents |
| title_sort | features of immune status in covid 19 convalescents |
| topic | covid-19 specific antibodies immune status |
| url | https://romj.org/node/468 |
| work_keys_str_mv | AT alinasshkaberda featuresofimmunestatusincovid19convalescents AT elenaakordovskaya featuresofimmunestatusincovid19convalescents AT dianayubakuta featuresofimmunestatusincovid19convalescents AT lyuboviplotnichenko featuresofimmunestatusincovid19convalescents AT alexanderagorbunov featuresofimmunestatusincovid19convalescents AT leyaesorokina featuresofimmunestatusincovid19convalescents |