Assessment of Alpha-Tocopheryl Acetate and Metformin Hydrochloride as Independent Agents on Human Dermal Fibroblast Viability: Findings from MTT Assay
Background: Alpha-tocopheryl acetate (Vitamin E) and metformin hydrochloride have been tested as anti-aging compounds at various concentrations. This study aimed to identify the most effective concentrations of alpha-tocopheryl acetate and metformin hydrochloride in promoting the viability of human...
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| Format: | Article |
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Universitas Padjadjaran
2025-06-01
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| Series: | Althea Medical Journal |
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| Online Access: | https://journal.fk.unpad.ac.id/index.php/amj/article/view/3954 |
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| author | Nayla Majeda Alfarafisa Astrid Feinisa Khairani Achadiyani Achadiyani Yoan Chou Fitri Aulia Muhammad Bintang Firdaus |
| author_facet | Nayla Majeda Alfarafisa Astrid Feinisa Khairani Achadiyani Achadiyani Yoan Chou Fitri Aulia Muhammad Bintang Firdaus |
| author_sort | Nayla Majeda Alfarafisa |
| collection | DOAJ |
| description | Background: Alpha-tocopheryl acetate (Vitamin E) and metformin hydrochloride have been tested as anti-aging compounds at various concentrations. This study aimed to identify the most effective concentrations of alpha-tocopheryl acetate and metformin hydrochloride in promoting the viability of human dermal fibroblasts (HDFs), a primary cell type in skin aging research.
Methods: HDFs were isolated using a mechanical isolation method and cultured under standard conditions. Cells were treated with varying concentrations of alpha-tocopheryl acetate and metformin hydrochloride as independents agents. After 48 hours of incubation, cell viability was measured using the MTT assay.
Results: Alpha-tocopheryl acetate had the highest HDF cell viability (107%) at a concentration of 50 μM. Metformin hydrochloride had the maximum HDF cell viability (158%) at 5 μM. However, the viability response varied across different concentrations for both agents, indicating that optimal dosing was essential for maximizing their effectiveness.
Conclusions: Alpha-tocopheryl acetate at 50 μM and metformin hydrochloride at 50 μM yield the highest viability of HDFs in vitro. These findings suggest potential roles for both agents in anti-aging skin therapies. Further research is recommended to explore their mechanisms of action and to optimize dosing strategies for clinical applications. |
| format | Article |
| id | doaj-art-1d2d23d6950944cfa28563ff334dbfa6 |
| institution | Kabale University |
| issn | 2337-4330 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Universitas Padjadjaran |
| record_format | Article |
| series | Althea Medical Journal |
| spelling | doaj-art-1d2d23d6950944cfa28563ff334dbfa62025-08-20T03:58:11ZengUniversitas PadjadjaranAlthea Medical Journal2337-43302025-06-0112212513210.15850/amj.v12n2.39541865Assessment of Alpha-Tocopheryl Acetate and Metformin Hydrochloride as Independent Agents on Human Dermal Fibroblast Viability: Findings from MTT AssayNayla Majeda Alfarafisa0Astrid Feinisa Khairani1Achadiyani Achadiyani2Yoan Chou3Fitri Aulia4Muhammad Bintang Firdaus5Department of Biomedical Sciences, Faculty of Medicine, Universitas PadjadjaranDepartment of Biomedical Sciences, Faculty of Medicine, Universitas PadjadjaranDepartment of Biomedical Sciences, Faculty of Medicine, Universitas PadjadjaranMaster Program in Anti-aging and Aesthetic Medicine, Faculty of Medicine, Universitas PadjadjaranMaster Program in Anti-aging and Aesthetic Medicine, Faculty of Medicine, Universitas PadjadjaranFaculty of Medicine Universitas PadjadjaranBackground: Alpha-tocopheryl acetate (Vitamin E) and metformin hydrochloride have been tested as anti-aging compounds at various concentrations. This study aimed to identify the most effective concentrations of alpha-tocopheryl acetate and metformin hydrochloride in promoting the viability of human dermal fibroblasts (HDFs), a primary cell type in skin aging research. Methods: HDFs were isolated using a mechanical isolation method and cultured under standard conditions. Cells were treated with varying concentrations of alpha-tocopheryl acetate and metformin hydrochloride as independents agents. After 48 hours of incubation, cell viability was measured using the MTT assay. Results: Alpha-tocopheryl acetate had the highest HDF cell viability (107%) at a concentration of 50 μM. Metformin hydrochloride had the maximum HDF cell viability (158%) at 5 μM. However, the viability response varied across different concentrations for both agents, indicating that optimal dosing was essential for maximizing their effectiveness. Conclusions: Alpha-tocopheryl acetate at 50 μM and metformin hydrochloride at 50 μM yield the highest viability of HDFs in vitro. These findings suggest potential roles for both agents in anti-aging skin therapies. Further research is recommended to explore their mechanisms of action and to optimize dosing strategies for clinical applications.https://journal.fk.unpad.ac.id/index.php/amj/article/view/3954alpha-tocopheryl acetate, anti-aging, cell viability, metformin hydrocholride, mtt assay |
| spellingShingle | Nayla Majeda Alfarafisa Astrid Feinisa Khairani Achadiyani Achadiyani Yoan Chou Fitri Aulia Muhammad Bintang Firdaus Assessment of Alpha-Tocopheryl Acetate and Metformin Hydrochloride as Independent Agents on Human Dermal Fibroblast Viability: Findings from MTT Assay Althea Medical Journal alpha-tocopheryl acetate, anti-aging, cell viability, metformin hydrocholride, mtt assay |
| title | Assessment of Alpha-Tocopheryl Acetate and Metformin Hydrochloride as Independent Agents on Human Dermal Fibroblast Viability: Findings from MTT Assay |
| title_full | Assessment of Alpha-Tocopheryl Acetate and Metformin Hydrochloride as Independent Agents on Human Dermal Fibroblast Viability: Findings from MTT Assay |
| title_fullStr | Assessment of Alpha-Tocopheryl Acetate and Metformin Hydrochloride as Independent Agents on Human Dermal Fibroblast Viability: Findings from MTT Assay |
| title_full_unstemmed | Assessment of Alpha-Tocopheryl Acetate and Metformin Hydrochloride as Independent Agents on Human Dermal Fibroblast Viability: Findings from MTT Assay |
| title_short | Assessment of Alpha-Tocopheryl Acetate and Metformin Hydrochloride as Independent Agents on Human Dermal Fibroblast Viability: Findings from MTT Assay |
| title_sort | assessment of alpha tocopheryl acetate and metformin hydrochloride as independent agents on human dermal fibroblast viability findings from mtt assay |
| topic | alpha-tocopheryl acetate, anti-aging, cell viability, metformin hydrocholride, mtt assay |
| url | https://journal.fk.unpad.ac.id/index.php/amj/article/view/3954 |
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