The Inhibitory Effect of PIK-75 on Inflammatory Mediator Response Induced by Hydrogen Peroxide in Feline Esophageal Epithelial Cells
Isoform-selective inhibitors of phosphoinositide 3-kinase (PI3K) activation have an anti-inflammatory effect by reducing proinflammatory cytokines. Cultured feline esophageal epithelial cells (EEC) of passages 3~4 were treated with hydrogen peroxide and PIK-75. The cell viability was measured by a M...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2014/178049 |
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| author | Jun Yeong Jeong Yeon Joo Lee Jeong Hoon Han Sun Young Park Kwang Woo Hwang Uy Dong Sohn |
| author_facet | Jun Yeong Jeong Yeon Joo Lee Jeong Hoon Han Sun Young Park Kwang Woo Hwang Uy Dong Sohn |
| author_sort | Jun Yeong Jeong |
| collection | DOAJ |
| description | Isoform-selective inhibitors of phosphoinositide 3-kinase (PI3K) activation have an anti-inflammatory effect by reducing proinflammatory cytokines. Cultured feline esophageal epithelial cells (EEC) of passages 3~4 were treated with hydrogen peroxide and PIK-75. The cell viability was measured by a MTT incorporation assay. The distribution of PI3K isoforms, p-Akt, IL-1β, and IL-8 was inferred from Western blots. The release of IL-6 was determined by ELISA. The cell morphology was not considerably different from nontreated cells if the cells were pretreated with PIK-75 and treated with 300 μM hydrogen peroxide. The density of p110α of PI3K was increased, but that of other types was not affected after the treatment with hydrogen peroxide. The density of p-Akt, when the cells were exposed to PIK-75 and hydrogen peroxide, was diminished dose dependently more than that of hydrogen peroxide treatment only. The decrease of p-Akt showed an inhibition of PI3K by PIK-75. PIK-75 dose dependently reduced the expression of IL-1β, IL-8, and the level of IL-6 compared with hydrogen peroxide treatment only. These results suggest evidence that p110α mediates esophageal inflammation and that PIK-75 has an anti-inflammatory effect by reducing proinflammatory cytokines on feline esophageal epithelial cultured cells. |
| format | Article |
| id | doaj-art-1d22bda939ea43ee806dbca42db75f3e |
| institution | OA Journals |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-1d22bda939ea43ee806dbca42db75f3e2025-08-20T02:01:39ZengWileyMediators of Inflammation0962-93511466-18612014-01-01201410.1155/2014/178049178049The Inhibitory Effect of PIK-75 on Inflammatory Mediator Response Induced by Hydrogen Peroxide in Feline Esophageal Epithelial CellsJun Yeong Jeong0Yeon Joo Lee1Jeong Hoon Han2Sun Young Park3Kwang Woo Hwang4Uy Dong Sohn5Signaling and Pharmacological Activity Research Lab, College of Pharmacy, Chung-Ang University, Seoul 156-756, Republic of KoreaSignaling and Pharmacological Activity Research Lab, College of Pharmacy, Chung-Ang University, Seoul 156-756, Republic of KoreaSignaling and Pharmacological Activity Research Lab, College of Pharmacy, Chung-Ang University, Seoul 156-756, Republic of KoreaSignaling and Pharmacological Activity Research Lab, College of Pharmacy, Chung-Ang University, Seoul 156-756, Republic of KoreaHost Defense Modulation Lab, College of Pharmacy, Chung-Ang University, Seoul 156-756, Republic of KoreaSignaling and Pharmacological Activity Research Lab, College of Pharmacy, Chung-Ang University, Seoul 156-756, Republic of KoreaIsoform-selective inhibitors of phosphoinositide 3-kinase (PI3K) activation have an anti-inflammatory effect by reducing proinflammatory cytokines. Cultured feline esophageal epithelial cells (EEC) of passages 3~4 were treated with hydrogen peroxide and PIK-75. The cell viability was measured by a MTT incorporation assay. The distribution of PI3K isoforms, p-Akt, IL-1β, and IL-8 was inferred from Western blots. The release of IL-6 was determined by ELISA. The cell morphology was not considerably different from nontreated cells if the cells were pretreated with PIK-75 and treated with 300 μM hydrogen peroxide. The density of p110α of PI3K was increased, but that of other types was not affected after the treatment with hydrogen peroxide. The density of p-Akt, when the cells were exposed to PIK-75 and hydrogen peroxide, was diminished dose dependently more than that of hydrogen peroxide treatment only. The decrease of p-Akt showed an inhibition of PI3K by PIK-75. PIK-75 dose dependently reduced the expression of IL-1β, IL-8, and the level of IL-6 compared with hydrogen peroxide treatment only. These results suggest evidence that p110α mediates esophageal inflammation and that PIK-75 has an anti-inflammatory effect by reducing proinflammatory cytokines on feline esophageal epithelial cultured cells.http://dx.doi.org/10.1155/2014/178049 |
| spellingShingle | Jun Yeong Jeong Yeon Joo Lee Jeong Hoon Han Sun Young Park Kwang Woo Hwang Uy Dong Sohn The Inhibitory Effect of PIK-75 on Inflammatory Mediator Response Induced by Hydrogen Peroxide in Feline Esophageal Epithelial Cells Mediators of Inflammation |
| title | The Inhibitory Effect of PIK-75 on Inflammatory Mediator Response Induced by Hydrogen Peroxide in Feline Esophageal Epithelial Cells |
| title_full | The Inhibitory Effect of PIK-75 on Inflammatory Mediator Response Induced by Hydrogen Peroxide in Feline Esophageal Epithelial Cells |
| title_fullStr | The Inhibitory Effect of PIK-75 on Inflammatory Mediator Response Induced by Hydrogen Peroxide in Feline Esophageal Epithelial Cells |
| title_full_unstemmed | The Inhibitory Effect of PIK-75 on Inflammatory Mediator Response Induced by Hydrogen Peroxide in Feline Esophageal Epithelial Cells |
| title_short | The Inhibitory Effect of PIK-75 on Inflammatory Mediator Response Induced by Hydrogen Peroxide in Feline Esophageal Epithelial Cells |
| title_sort | inhibitory effect of pik 75 on inflammatory mediator response induced by hydrogen peroxide in feline esophageal epithelial cells |
| url | http://dx.doi.org/10.1155/2014/178049 |
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