Correlation Analysis between Gut Microbiota and Metabolites in Children with Systemic Lupus Erythematosus
Systemic lupus erythematosus (SLE) is an autoimmune-mediated diffuse connective tissue disease characterized by immune inflammation with an unclear aetiology and pathogenesis. This work profiled the intestinal flora and faecal metabolome of patients with SLE using 16S RNA sequencing and gas chromato...
Saved in:
| Main Authors: | , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2021-01-01
|
| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2021/5579608 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850237420622053376 |
|---|---|
| author | Min Wen Taohua Liu Mingyi Zhao Xiqiang Dang Shipin Feng Xuewei Ding Zhiquan Xu Xiaoyan Huang Qiuyu Lin Wei Xiang Xiaoyan Li Xiaojie He Qingnan He |
| author_facet | Min Wen Taohua Liu Mingyi Zhao Xiqiang Dang Shipin Feng Xuewei Ding Zhiquan Xu Xiaoyan Huang Qiuyu Lin Wei Xiang Xiaoyan Li Xiaojie He Qingnan He |
| author_sort | Min Wen |
| collection | DOAJ |
| description | Systemic lupus erythematosus (SLE) is an autoimmune-mediated diffuse connective tissue disease characterized by immune inflammation with an unclear aetiology and pathogenesis. This work profiled the intestinal flora and faecal metabolome of patients with SLE using 16S RNA sequencing and gas chromatography-mass spectrometry (GC-MS). We identified unchanged alpha diversity and partially altered beta diversity of the intestinal flora. Another important finding was the increase in Proteobacteria and Enterobacteriales and the decrease in Ruminococcaceae among SLE patients. For metabolites, amino acids and short-chain fatty acids were enriched when long-chain fatty acids were downregulated in SLE faecal samples. KEGG analysis showed the significance of the protein digestion and absorption pathway, and association analysis revealed the key role of 3-phenylpropanoic acid and Sphingomonas. Sphingomonas were reported to be less abundant in healthy periodontal sites of SLE patients than in those of HCs, indicating transmission of oral species to the gut. This study contributes to the understanding of the pathogenesis of SLE disease from the perspective of intestinal microorganisms, explains the pathogenesis of SLE, and serves as a basis for exploring potential treatments for the disease. |
| format | Article |
| id | doaj-art-1d06f0af77cb429f91b25d723cd0b656 |
| institution | OA Journals |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-1d06f0af77cb429f91b25d723cd0b6562025-08-20T02:01:43ZengWileyJournal of Immunology Research2314-88612314-71562021-01-01202110.1155/2021/55796085579608Correlation Analysis between Gut Microbiota and Metabolites in Children with Systemic Lupus ErythematosusMin Wen0Taohua Liu1Mingyi Zhao2Xiqiang Dang3Shipin Feng4Xuewei Ding5Zhiquan Xu6Xiaoyan Huang7Qiuyu Lin8Wei Xiang9Xiaoyan Li10Xiaojie He11Qingnan He12Institute of Pediatrics, The Second Xiangya Hospital, Central South University, Changsha, ChinaDepartment of Pediatrics, The Third Xiangya Hospital, Central South University, Changsha, Hunan, ChinaDepartment of Pediatrics, The Third Xiangya Hospital, Central South University, Changsha, Hunan, ChinaInstitute of Pediatrics, The Second Xiangya Hospital, Central South University, Changsha, ChinaDepartment of Pediatric Nephrology, Chengdu Women’s and Children’s Central Hospital, School of Medicine, University of Electronic Science and Technology of China, Chengdu, Sichuan, ChinaInstitute of Pediatrics, The Second Xiangya Hospital, Central South University, Changsha, ChinaHainan Maternal and Children’s Medical Center, Haikou, Hainan, ChinaHainan Maternal and Children’s Medical Center, Haikou, Hainan, ChinaHainan Maternal and Children’s Medical Center, Haikou, Hainan, ChinaHainan Maternal and Children’s Medical Center, Haikou, Hainan, ChinaInstitute of Pediatrics, The Second Xiangya Hospital, Central South University, Changsha, ChinaInstitute of Pediatrics, The Second Xiangya Hospital, Central South University, Changsha, ChinaInstitute of Pediatrics, The Second Xiangya Hospital, Central South University, Changsha, ChinaSystemic lupus erythematosus (SLE) is an autoimmune-mediated diffuse connective tissue disease characterized by immune inflammation with an unclear aetiology and pathogenesis. This work profiled the intestinal flora and faecal metabolome of patients with SLE using 16S RNA sequencing and gas chromatography-mass spectrometry (GC-MS). We identified unchanged alpha diversity and partially altered beta diversity of the intestinal flora. Another important finding was the increase in Proteobacteria and Enterobacteriales and the decrease in Ruminococcaceae among SLE patients. For metabolites, amino acids and short-chain fatty acids were enriched when long-chain fatty acids were downregulated in SLE faecal samples. KEGG analysis showed the significance of the protein digestion and absorption pathway, and association analysis revealed the key role of 3-phenylpropanoic acid and Sphingomonas. Sphingomonas were reported to be less abundant in healthy periodontal sites of SLE patients than in those of HCs, indicating transmission of oral species to the gut. This study contributes to the understanding of the pathogenesis of SLE disease from the perspective of intestinal microorganisms, explains the pathogenesis of SLE, and serves as a basis for exploring potential treatments for the disease.http://dx.doi.org/10.1155/2021/5579608 |
| spellingShingle | Min Wen Taohua Liu Mingyi Zhao Xiqiang Dang Shipin Feng Xuewei Ding Zhiquan Xu Xiaoyan Huang Qiuyu Lin Wei Xiang Xiaoyan Li Xiaojie He Qingnan He Correlation Analysis between Gut Microbiota and Metabolites in Children with Systemic Lupus Erythematosus Journal of Immunology Research |
| title | Correlation Analysis between Gut Microbiota and Metabolites in Children with Systemic Lupus Erythematosus |
| title_full | Correlation Analysis between Gut Microbiota and Metabolites in Children with Systemic Lupus Erythematosus |
| title_fullStr | Correlation Analysis between Gut Microbiota and Metabolites in Children with Systemic Lupus Erythematosus |
| title_full_unstemmed | Correlation Analysis between Gut Microbiota and Metabolites in Children with Systemic Lupus Erythematosus |
| title_short | Correlation Analysis between Gut Microbiota and Metabolites in Children with Systemic Lupus Erythematosus |
| title_sort | correlation analysis between gut microbiota and metabolites in children with systemic lupus erythematosus |
| url | http://dx.doi.org/10.1155/2021/5579608 |
| work_keys_str_mv | AT minwen correlationanalysisbetweengutmicrobiotaandmetabolitesinchildrenwithsystemiclupuserythematosus AT taohualiu correlationanalysisbetweengutmicrobiotaandmetabolitesinchildrenwithsystemiclupuserythematosus AT mingyizhao correlationanalysisbetweengutmicrobiotaandmetabolitesinchildrenwithsystemiclupuserythematosus AT xiqiangdang correlationanalysisbetweengutmicrobiotaandmetabolitesinchildrenwithsystemiclupuserythematosus AT shipinfeng correlationanalysisbetweengutmicrobiotaandmetabolitesinchildrenwithsystemiclupuserythematosus AT xueweiding correlationanalysisbetweengutmicrobiotaandmetabolitesinchildrenwithsystemiclupuserythematosus AT zhiquanxu correlationanalysisbetweengutmicrobiotaandmetabolitesinchildrenwithsystemiclupuserythematosus AT xiaoyanhuang correlationanalysisbetweengutmicrobiotaandmetabolitesinchildrenwithsystemiclupuserythematosus AT qiuyulin correlationanalysisbetweengutmicrobiotaandmetabolitesinchildrenwithsystemiclupuserythematosus AT weixiang correlationanalysisbetweengutmicrobiotaandmetabolitesinchildrenwithsystemiclupuserythematosus AT xiaoyanli correlationanalysisbetweengutmicrobiotaandmetabolitesinchildrenwithsystemiclupuserythematosus AT xiaojiehe correlationanalysisbetweengutmicrobiotaandmetabolitesinchildrenwithsystemiclupuserythematosus AT qingnanhe correlationanalysisbetweengutmicrobiotaandmetabolitesinchildrenwithsystemiclupuserythematosus |