Silk Fibroin Microparticle/Carboxymethyl Cellulose Composite Gel for Wound Healing Applications
Silk fibroin has recently gained considerable attention as a promising biomaterial for use in medical and bioengineering technologies due to its biocompatibility and favorable mechanical properties. In this study, composite gel based on silk fibroin microparticles and carboxymethyl cellulose was dev...
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MDPI AG
2025-07-01
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| author | Alexander Pashutin Ekaterina Podbolotova Luidmila Kirsanova Onur Dosi Anton E. Efimov Olga Agapova Igor Agapov |
| author_facet | Alexander Pashutin Ekaterina Podbolotova Luidmila Kirsanova Onur Dosi Anton E. Efimov Olga Agapova Igor Agapov |
| author_sort | Alexander Pashutin |
| collection | DOAJ |
| description | Silk fibroin has recently gained considerable attention as a promising biomaterial for use in medical and bioengineering technologies due to its biocompatibility and favorable mechanical properties. In this study, composite gel based on silk fibroin microparticles and carboxymethyl cellulose was developed, characterized by a viscous, homogeneous white mass containing uniformly distributed fibroin microparticles ranging from 1 to 20 μm in size. The gel exhibited a kinematic viscosity of 36.5 × 10<sup>−6</sup> St, allowing for convenient application to wounds using a syringe or spatula while preventing uncontrolled spreading. The cytocompatibility of the gel was confirmed using the methylthiazol tetrazolium (MTT) assay, which showed no cytotoxic effects on 3T3 fibroblast cells. Furthermore, the gel remained stable for over one year when stored at 10 °C, in contrast to conventional fibroin solutions, which typically lose stability within a month under similar conditions. In a full-thickness skin wound model in rats, the application of the gel significantly accelerated skin regeneration, with complete wound closure observed by day 15, compared with 30 days in the control group. Histological analysis confirmed the restoration of all skin layers. These findings demonstrate the high potential of the gel for applications in regenerative medicine and tissue engineering. |
| format | Article |
| id | doaj-art-1cff42e0f0744ee299fdb466a370daad |
| institution | DOAJ |
| issn | 2313-7673 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | MDPI AG |
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| series | Biomimetics |
| spelling | doaj-art-1cff42e0f0744ee299fdb466a370daad2025-08-20T03:07:54ZengMDPI AGBiomimetics2313-76732025-07-0110743410.3390/biomimetics10070434Silk Fibroin Microparticle/Carboxymethyl Cellulose Composite Gel for Wound Healing ApplicationsAlexander Pashutin0Ekaterina Podbolotova1Luidmila Kirsanova2Onur Dosi3Anton E. Efimov4Olga Agapova5Igor Agapov6Academician V.I. Shumakov National Medical Research Center of Transplantology and Artificial Organs, Ministry of Health of the Russian Federation, 123182 Moscow, RussiaAcademician V.I. Shumakov National Medical Research Center of Transplantology and Artificial Organs, Ministry of Health of the Russian Federation, 123182 Moscow, RussiaAcademician V.I. Shumakov National Medical Research Center of Transplantology and Artificial Organs, Ministry of Health of the Russian Federation, 123182 Moscow, RussiaAcademician V.I. Shumakov National Medical Research Center of Transplantology and Artificial Organs, Ministry of Health of the Russian Federation, 123182 Moscow, RussiaAcademician V.I. Shumakov National Medical Research Center of Transplantology and Artificial Organs, Ministry of Health of the Russian Federation, 123182 Moscow, RussiaAcademician V.I. Shumakov National Medical Research Center of Transplantology and Artificial Organs, Ministry of Health of the Russian Federation, 123182 Moscow, RussiaAcademician V.I. Shumakov National Medical Research Center of Transplantology and Artificial Organs, Ministry of Health of the Russian Federation, 123182 Moscow, RussiaSilk fibroin has recently gained considerable attention as a promising biomaterial for use in medical and bioengineering technologies due to its biocompatibility and favorable mechanical properties. In this study, composite gel based on silk fibroin microparticles and carboxymethyl cellulose was developed, characterized by a viscous, homogeneous white mass containing uniformly distributed fibroin microparticles ranging from 1 to 20 μm in size. The gel exhibited a kinematic viscosity of 36.5 × 10<sup>−6</sup> St, allowing for convenient application to wounds using a syringe or spatula while preventing uncontrolled spreading. The cytocompatibility of the gel was confirmed using the methylthiazol tetrazolium (MTT) assay, which showed no cytotoxic effects on 3T3 fibroblast cells. Furthermore, the gel remained stable for over one year when stored at 10 °C, in contrast to conventional fibroin solutions, which typically lose stability within a month under similar conditions. In a full-thickness skin wound model in rats, the application of the gel significantly accelerated skin regeneration, with complete wound closure observed by day 15, compared with 30 days in the control group. Histological analysis confirmed the restoration of all skin layers. These findings demonstrate the high potential of the gel for applications in regenerative medicine and tissue engineering.https://www.mdpi.com/2313-7673/10/7/434silk fibroin microparticlescarboxymethyl cellulosecomposite hydrogelwound healingcytocompatibility |
| spellingShingle | Alexander Pashutin Ekaterina Podbolotova Luidmila Kirsanova Onur Dosi Anton E. Efimov Olga Agapova Igor Agapov Silk Fibroin Microparticle/Carboxymethyl Cellulose Composite Gel for Wound Healing Applications Biomimetics silk fibroin microparticles carboxymethyl cellulose composite hydrogel wound healing cytocompatibility |
| title | Silk Fibroin Microparticle/Carboxymethyl Cellulose Composite Gel for Wound Healing Applications |
| title_full | Silk Fibroin Microparticle/Carboxymethyl Cellulose Composite Gel for Wound Healing Applications |
| title_fullStr | Silk Fibroin Microparticle/Carboxymethyl Cellulose Composite Gel for Wound Healing Applications |
| title_full_unstemmed | Silk Fibroin Microparticle/Carboxymethyl Cellulose Composite Gel for Wound Healing Applications |
| title_short | Silk Fibroin Microparticle/Carboxymethyl Cellulose Composite Gel for Wound Healing Applications |
| title_sort | silk fibroin microparticle carboxymethyl cellulose composite gel for wound healing applications |
| topic | silk fibroin microparticles carboxymethyl cellulose composite hydrogel wound healing cytocompatibility |
| url | https://www.mdpi.com/2313-7673/10/7/434 |
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