Allele variants of HLA II genes DRB1 and DQB1 regarding risk for type 1 diabetes mellitus in population of Bashkortostan
Aims. To estimate significance of HLA II DRB1 and DRB2 allele variants for development of type 1 diabetes mellitus (T1DM) in Bashkortostanpopulation (ethnical Russians, Tatar, Bashkir). Materials and methods. We analyzed DNA of 323 patients with T1DM and 683 healthy controls. DNA was derived from...
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Endocrinology Research Centre
2012-09-01
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| Series: | Сахарный диабет |
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| Online Access: | https://www.dia-endojournals.ru/jour/article/view/6081 |
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| author | Shamilevna Avzaletdinova Tatiana Vyacheslavovna Morugova Olga Evgen'evna Mustafina |
| author_facet | Shamilevna Avzaletdinova Tatiana Vyacheslavovna Morugova Olga Evgen'evna Mustafina |
| author_sort | Shamilevna Avzaletdinova |
| collection | DOAJ |
| description | Aims. To estimate significance of HLA II DRB1 and DRB2 allele variants for development of type 1 diabetes mellitus (T1DM) in Bashkortostanpopulation (ethnical Russians, Tatar, Bashkir). Materials and methods. We analyzed DNA of 323 patients with T1DM and 683 healthy controls. DNA was derived from venous bloodsamples by phenol-chloroform extraction. DRB1 and DQB1 gene typing was performed by PCR method. Amplification products wereidentified with electrophoresis on a 1% agarose gel. Statistica for Windows v6.0 and MS Excel 98 software were applied for statisticalprocessing of acquired data. Results. Common markers of high risk for T1DM were found to be DRB1*04, DRB1*17, genotype DRB1*04/*17. On the contrary,lower risk was associated with DRB1*15 allele. In ethnical Russians lower risk of T1DM is also determined by DRB1*11 allele andDRB1*01 in Tatars. Predisposition by DQB1-alleles in Russians and Bashkir realizes only within DRB1*04/*17 genotype. However,in Tatar subpopulation DQB1*0302 is an independent risk marker of T1DM development. Conclusion. Common low risk markers for all three ethnic groups are DQB1*0301, DQB1*0602-08 alleles. Their presence negates riskof disease in all studied subpopulations even within DRB1*04/*17-genotype. |
| format | Article |
| id | doaj-art-1cfca0c45ec446feb08d3a620c2af1c1 |
| institution | Kabale University |
| issn | 2072-0351 2072-0378 |
| language | English |
| publishDate | 2012-09-01 |
| publisher | Endocrinology Research Centre |
| record_format | Article |
| series | Сахарный диабет |
| spelling | doaj-art-1cfca0c45ec446feb08d3a620c2af1c12025-08-20T03:47:54ZengEndocrinology Research CentreСахарный диабет2072-03512072-03782012-09-01153182310.14341/2072-0351-60816039Allele variants of HLA II genes DRB1 and DQB1 regarding risk for type 1 diabetes mellitus in population of BashkortostanShamilevna Avzaletdinova0Tatiana Vyacheslavovna Morugova1Olga Evgen'evna Mustafina2Bashkir State Medical University, Ufa, Russian FederationBashkir State Medical University, Ufa, Russian FederationDepartment of Biochemistry and Genetics, Ufa, Russian FederationAims. To estimate significance of HLA II DRB1 and DRB2 allele variants for development of type 1 diabetes mellitus (T1DM) in Bashkortostanpopulation (ethnical Russians, Tatar, Bashkir). Materials and methods. We analyzed DNA of 323 patients with T1DM and 683 healthy controls. DNA was derived from venous bloodsamples by phenol-chloroform extraction. DRB1 and DQB1 gene typing was performed by PCR method. Amplification products wereidentified with electrophoresis on a 1% agarose gel. Statistica for Windows v6.0 and MS Excel 98 software were applied for statisticalprocessing of acquired data. Results. Common markers of high risk for T1DM were found to be DRB1*04, DRB1*17, genotype DRB1*04/*17. On the contrary,lower risk was associated with DRB1*15 allele. In ethnical Russians lower risk of T1DM is also determined by DRB1*11 allele andDRB1*01 in Tatars. Predisposition by DQB1-alleles in Russians and Bashkir realizes only within DRB1*04/*17 genotype. However,in Tatar subpopulation DQB1*0302 is an independent risk marker of T1DM development. Conclusion. Common low risk markers for all three ethnic groups are DQB1*0301, DQB1*0602-08 alleles. Their presence negates riskof disease in all studied subpopulations even within DRB1*04/*17-genotype.https://www.dia-endojournals.ru/jour/article/view/6081diabetes mellitus type 1genetic predispositionhla |
| spellingShingle | Shamilevna Avzaletdinova Tatiana Vyacheslavovna Morugova Olga Evgen'evna Mustafina Allele variants of HLA II genes DRB1 and DQB1 regarding risk for type 1 diabetes mellitus in population of Bashkortostan Сахарный диабет diabetes mellitus type 1 genetic predisposition hla |
| title | Allele variants of HLA II genes DRB1 and DQB1 regarding risk for type 1 diabetes mellitus in population of Bashkortostan |
| title_full | Allele variants of HLA II genes DRB1 and DQB1 regarding risk for type 1 diabetes mellitus in population of Bashkortostan |
| title_fullStr | Allele variants of HLA II genes DRB1 and DQB1 regarding risk for type 1 diabetes mellitus in population of Bashkortostan |
| title_full_unstemmed | Allele variants of HLA II genes DRB1 and DQB1 regarding risk for type 1 diabetes mellitus in population of Bashkortostan |
| title_short | Allele variants of HLA II genes DRB1 and DQB1 regarding risk for type 1 diabetes mellitus in population of Bashkortostan |
| title_sort | allele variants of hla ii genes drb1 and dqb1 regarding risk for type 1 diabetes mellitus in population of bashkortostan |
| topic | diabetes mellitus type 1 genetic predisposition hla |
| url | https://www.dia-endojournals.ru/jour/article/view/6081 |
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