Molecular identification and artificial cultivation of a wild isolate of oyster mushroom in Albania
Basidiomata of a wild mushroom macroscopically recognised as Pleurotus ostreatus were observed on an oak trunk in a mixed wood of northern Albania. Pure cultures of the fungus were then obtained on potato-dextrose-agar medium. Molecular analyses of genomic DNA of the fungus confirmed its identificat...
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| Format: | Article |
| Language: | English |
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Elsevier
2016-03-01
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| Series: | Italian Journal of Agronomy |
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| Online Access: | https://www.agronomy.it/index.php/agro/article/view/704 |
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| author | Jordan Merkuri Stefania Mirela Mang Ippolito Camele Magdalena Cara Gian Luigi Rana |
| author_facet | Jordan Merkuri Stefania Mirela Mang Ippolito Camele Magdalena Cara Gian Luigi Rana |
| author_sort | Jordan Merkuri |
| collection | DOAJ |
| description | Basidiomata of a wild mushroom macroscopically recognised as Pleurotus ostreatus were observed on an oak trunk in a mixed wood of northern Albania. Pure cultures of the fungus were then obtained on potato-dextrose-agar medium. Molecular analyses of genomic DNA of the fungus confirmed its identification. The rDNA ITS region nucleotide sequence of the studied Pleurotacea matched at 99% those of two P. ostreatus strains already present in NCBI GenBank database. The rDNA ITS nucelotide sequences of two pure cultures of the Albanian P. ostreatus were deposited in EMBL database under the accession numbers LN849458 and LN849459. One of the fungus isolates was subsequently cultivated under protected and semi-natural conditions. Productivity and biological efficiency of the Albanian P. ostreatus ranged from about 10% to 16% and from 33 to 53.33%, respectively. This seems to be the first report on the artificial cultivation of P. ostreatus in Albania and could have, in the next future, a high economic impact on development and diffusion of this important edible mushroom over the country. |
| format | Article |
| id | doaj-art-1cf405fe70c64756a2f618fe75bd8a2f |
| institution | OA Journals |
| issn | 1125-4718 2039-6805 |
| language | English |
| publishDate | 2016-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Italian Journal of Agronomy |
| spelling | doaj-art-1cf405fe70c64756a2f618fe75bd8a2f2025-08-20T01:55:01ZengElsevierItalian Journal of Agronomy1125-47182039-68052016-03-0111110.4081/ija.2016.704534Molecular identification and artificial cultivation of a wild isolate of oyster mushroom in AlbaniaJordan Merkuri0Stefania Mirela Mang1Ippolito Camele2Magdalena Cara3Gian Luigi Rana4Department of Plant Protection, Agricultural University of Tirana, TiranaSchool of Agricultural, Forestry, Food and Environmental Sciences, University of Basilicata, PotenzaSchool of Agricultural, Forestry, Food and Environmental Sciences, University of Basilicata, PotenzaDepartment of Plant Protection, Agricultural University of Tirana, TiranaSchool of Agricultural, Forestry, Food and Environmental Sciences, University of Basilicata, PotenzaBasidiomata of a wild mushroom macroscopically recognised as Pleurotus ostreatus were observed on an oak trunk in a mixed wood of northern Albania. Pure cultures of the fungus were then obtained on potato-dextrose-agar medium. Molecular analyses of genomic DNA of the fungus confirmed its identification. The rDNA ITS region nucleotide sequence of the studied Pleurotacea matched at 99% those of two P. ostreatus strains already present in NCBI GenBank database. The rDNA ITS nucelotide sequences of two pure cultures of the Albanian P. ostreatus were deposited in EMBL database under the accession numbers LN849458 and LN849459. One of the fungus isolates was subsequently cultivated under protected and semi-natural conditions. Productivity and biological efficiency of the Albanian P. ostreatus ranged from about 10% to 16% and from 33 to 53.33%, respectively. This seems to be the first report on the artificial cultivation of P. ostreatus in Albania and could have, in the next future, a high economic impact on development and diffusion of this important edible mushroom over the country.https://www.agronomy.it/index.php/agro/article/view/704Cultivationmolecular analysispolymerase chain reactionPleurotus ostreatussubstrate pasteurisation. |
| spellingShingle | Jordan Merkuri Stefania Mirela Mang Ippolito Camele Magdalena Cara Gian Luigi Rana Molecular identification and artificial cultivation of a wild isolate of oyster mushroom in Albania Italian Journal of Agronomy Cultivation molecular analysis polymerase chain reaction Pleurotus ostreatus substrate pasteurisation. |
| title | Molecular identification and artificial cultivation of a wild isolate of oyster mushroom in Albania |
| title_full | Molecular identification and artificial cultivation of a wild isolate of oyster mushroom in Albania |
| title_fullStr | Molecular identification and artificial cultivation of a wild isolate of oyster mushroom in Albania |
| title_full_unstemmed | Molecular identification and artificial cultivation of a wild isolate of oyster mushroom in Albania |
| title_short | Molecular identification and artificial cultivation of a wild isolate of oyster mushroom in Albania |
| title_sort | molecular identification and artificial cultivation of a wild isolate of oyster mushroom in albania |
| topic | Cultivation molecular analysis polymerase chain reaction Pleurotus ostreatus substrate pasteurisation. |
| url | https://www.agronomy.it/index.php/agro/article/view/704 |
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