In Silico Development of a Multi-Epitope Subunit Vaccine against Bluetongue Virus in <i>Ovis aries</i> Using Immunoinformatics
The bluetongue virus (BTV), transmitted by biting midges, poses a significant threat to livestock globally. This orbivirus induces bluetongue disease, leading to substantial economic losses in the agricultural sector. The current control measures have limitations, necessitating the development of no...
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MDPI AG
2024-10-01
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| author | Priyansha Raj Sinha Shubhada R. Hegde Ruchika Mittal Chikkamagaluru Chandrashekhar Jagat Ullas Gowda Rathna Chandrashekhar Gayathri Muthaiah Samer Shamshad Mohammed Mudassar Chanda Vishweshwar Ganji Kalyani Putty Divakar Hemadri |
| author_facet | Priyansha Raj Sinha Shubhada R. Hegde Ruchika Mittal Chikkamagaluru Chandrashekhar Jagat Ullas Gowda Rathna Chandrashekhar Gayathri Muthaiah Samer Shamshad Mohammed Mudassar Chanda Vishweshwar Ganji Kalyani Putty Divakar Hemadri |
| author_sort | Priyansha Raj Sinha |
| collection | DOAJ |
| description | The bluetongue virus (BTV), transmitted by biting midges, poses a significant threat to livestock globally. This orbivirus induces bluetongue disease, leading to substantial economic losses in the agricultural sector. The current control measures have limitations, necessitating the development of novel, efficient vaccines. In this study, an immunoinformatics approach is employed to design a multi-epitope subunit vaccine for <i>Ovis aries</i> targeting six BTV serotypes. Focusing on the VP2 capsid protein, the vaccine incorporates B-cell, helper-T lymphocytes (HTL), and cytotoxic T-cell lymphocytes (CTL) epitopes. Molecular docking reveals stable interactions with TLR2 and TLR4 receptors, suggesting the stability of the complex, indicating the potential viability of the multi-epitope vaccine. The computational approach offers a rapid and tailored strategy for vaccine development, highlighting potential efficacy and safety against BTV outbreaks. This work contributes to understanding BTV and presents a promising avenue for effective control. |
| format | Article |
| id | doaj-art-1cf1c66ac354463f91f5b391e88abddb |
| institution | DOAJ |
| issn | 2076-0817 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj-art-1cf1c66ac354463f91f5b391e88abddb2025-08-20T02:48:07ZengMDPI AGPathogens2076-08172024-10-01131194410.3390/pathogens13110944In Silico Development of a Multi-Epitope Subunit Vaccine against Bluetongue Virus in <i>Ovis aries</i> Using ImmunoinformaticsPriyansha Raj Sinha0Shubhada R. Hegde1Ruchika Mittal2Chikkamagaluru Chandrashekhar Jagat3Ullas Gowda4Rathna Chandrashekhar5Gayathri Muthaiah6Samer Shamshad7Mohammed Mudassar Chanda8Vishweshwar Ganji9Kalyani Putty10Divakar Hemadri11Institute of Bioinformatics and Applied Biotechnology (IBAB), Bengaluru 560100, IndiaInstitute of Bioinformatics and Applied Biotechnology (IBAB), Bengaluru 560100, IndiaInstitute of Bioinformatics and Applied Biotechnology (IBAB), Bengaluru 560100, IndiaICAR—National Institute of Veterinary Epidemiology and Disease Informatics, Bengaluru 560064, IndiaICAR—National Institute of Veterinary Epidemiology and Disease Informatics, Bengaluru 560064, IndiaICAR—National Institute of Veterinary Epidemiology and Disease Informatics, Bengaluru 560064, IndiaICAR—National Institute of Veterinary Epidemiology and Disease Informatics, Bengaluru 560064, IndiaICAR—National Institute of Veterinary Epidemiology and Disease Informatics, Bengaluru 560064, IndiaICAR—National Institute of Veterinary Epidemiology and Disease Informatics, Bengaluru 560064, IndiaDepartment of Veterinary Biotechnology, CoVsc, PVNRTVU, Hyderabad 500030, IndiaDepartment of Veterinary Biotechnology, CoVsc, PVNRTVU, Hyderabad 500030, IndiaICAR—National Institute of Veterinary Epidemiology and Disease Informatics, Bengaluru 560064, IndiaThe bluetongue virus (BTV), transmitted by biting midges, poses a significant threat to livestock globally. This orbivirus induces bluetongue disease, leading to substantial economic losses in the agricultural sector. The current control measures have limitations, necessitating the development of novel, efficient vaccines. In this study, an immunoinformatics approach is employed to design a multi-epitope subunit vaccine for <i>Ovis aries</i> targeting six BTV serotypes. Focusing on the VP2 capsid protein, the vaccine incorporates B-cell, helper-T lymphocytes (HTL), and cytotoxic T-cell lymphocytes (CTL) epitopes. Molecular docking reveals stable interactions with TLR2 and TLR4 receptors, suggesting the stability of the complex, indicating the potential viability of the multi-epitope vaccine. The computational approach offers a rapid and tailored strategy for vaccine development, highlighting potential efficacy and safety against BTV outbreaks. This work contributes to understanding BTV and presents a promising avenue for effective control.https://www.mdpi.com/2076-0817/13/11/944bluetongue virusepitopessubunit vaccine<i>Ovis aries</i> |
| spellingShingle | Priyansha Raj Sinha Shubhada R. Hegde Ruchika Mittal Chikkamagaluru Chandrashekhar Jagat Ullas Gowda Rathna Chandrashekhar Gayathri Muthaiah Samer Shamshad Mohammed Mudassar Chanda Vishweshwar Ganji Kalyani Putty Divakar Hemadri In Silico Development of a Multi-Epitope Subunit Vaccine against Bluetongue Virus in <i>Ovis aries</i> Using Immunoinformatics Pathogens bluetongue virus epitopes subunit vaccine <i>Ovis aries</i> |
| title | In Silico Development of a Multi-Epitope Subunit Vaccine against Bluetongue Virus in <i>Ovis aries</i> Using Immunoinformatics |
| title_full | In Silico Development of a Multi-Epitope Subunit Vaccine against Bluetongue Virus in <i>Ovis aries</i> Using Immunoinformatics |
| title_fullStr | In Silico Development of a Multi-Epitope Subunit Vaccine against Bluetongue Virus in <i>Ovis aries</i> Using Immunoinformatics |
| title_full_unstemmed | In Silico Development of a Multi-Epitope Subunit Vaccine against Bluetongue Virus in <i>Ovis aries</i> Using Immunoinformatics |
| title_short | In Silico Development of a Multi-Epitope Subunit Vaccine against Bluetongue Virus in <i>Ovis aries</i> Using Immunoinformatics |
| title_sort | in silico development of a multi epitope subunit vaccine against bluetongue virus in i ovis aries i using immunoinformatics |
| topic | bluetongue virus epitopes subunit vaccine <i>Ovis aries</i> |
| url | https://www.mdpi.com/2076-0817/13/11/944 |
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