Trypanosoma congolense Infections: Induced Nitric Oxide Inhibits Parasite Growth In Vivo
Wild-type (WT) C57BL/6 mice infected intraperitoneally with 5×106 Trypanosoma congolense survive for more than 30 days. C57BL/6 mice deficient in inducible nitric oxide synthase (iNOS−/−) and infected with 103 or 5×106 parasites do not control the parasitemia and survive for only 14±7 or 6.8±0.1 da...
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| Format: | Article |
| Language: | English |
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Wiley
2011-01-01
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| Series: | Journal of Parasitology Research |
| Online Access: | http://dx.doi.org/10.1155/2011/316067 |
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| author | Wenfa Lu Guojian Wei Wanling Pan Henry Tabel |
| author_facet | Wenfa Lu Guojian Wei Wanling Pan Henry Tabel |
| author_sort | Wenfa Lu |
| collection | DOAJ |
| description | Wild-type (WT) C57BL/6 mice infected intraperitoneally with 5×106 Trypanosoma congolense survive for more than 30 days. C57BL/6 mice deficient in inducible nitric oxide synthase (iNOS−/−) and infected with 103 or 5×106 parasites do not control the parasitemia and survive for only 14±7 or 6.8±0.1 days, respectively. Bloodstream trypanosomes of iNOS−/− mice infected with 5×106 T. congolense had a significantly higher ratio of organisms in the S+G2+M phases of the cell cycle than trypanosomes in WT mice. We have reported that IgM anti-VSG-mediated phagocytosis of T. congolense by macrophages inhibits nitric oxide (NO) synthesis via CR3 (CD11b/CD18). Here, we show that during the first parasitemia, but not at later stages of infection, T. congolense-infected CD11b−/− mice produce more NO and have a significantly lower parasitemia than infected WT mice. We conclude that induced NO contributes to the control of parasitemia by inhibiting the growth of the trypanosomes. |
| format | Article |
| id | doaj-art-1ce99f3547a64da08c85b752e07ea578 |
| institution | Kabale University |
| issn | 2090-0023 2090-0031 |
| language | English |
| publishDate | 2011-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Parasitology Research |
| spelling | doaj-art-1ce99f3547a64da08c85b752e07ea5782025-08-20T03:54:51ZengWileyJournal of Parasitology Research2090-00232090-00312011-01-01201110.1155/2011/316067316067Trypanosoma congolense Infections: Induced Nitric Oxide Inhibits Parasite Growth In VivoWenfa Lu0Guojian Wei1Wanling Pan2Henry Tabel3College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, ChinaDepartment of Veterinary Microbiology, Western College of Veterinary Medicine, University of Saskatchewan, 52 Campus Drive, Saskatoon, SK, S7N 5B4, CanadaDepartment of Veterinary Microbiology, Western College of Veterinary Medicine, University of Saskatchewan, 52 Campus Drive, Saskatoon, SK, S7N 5B4, CanadaDepartment of Veterinary Microbiology, Western College of Veterinary Medicine, University of Saskatchewan, 52 Campus Drive, Saskatoon, SK, S7N 5B4, CanadaWild-type (WT) C57BL/6 mice infected intraperitoneally with 5×106 Trypanosoma congolense survive for more than 30 days. C57BL/6 mice deficient in inducible nitric oxide synthase (iNOS−/−) and infected with 103 or 5×106 parasites do not control the parasitemia and survive for only 14±7 or 6.8±0.1 days, respectively. Bloodstream trypanosomes of iNOS−/− mice infected with 5×106 T. congolense had a significantly higher ratio of organisms in the S+G2+M phases of the cell cycle than trypanosomes in WT mice. We have reported that IgM anti-VSG-mediated phagocytosis of T. congolense by macrophages inhibits nitric oxide (NO) synthesis via CR3 (CD11b/CD18). Here, we show that during the first parasitemia, but not at later stages of infection, T. congolense-infected CD11b−/− mice produce more NO and have a significantly lower parasitemia than infected WT mice. We conclude that induced NO contributes to the control of parasitemia by inhibiting the growth of the trypanosomes.http://dx.doi.org/10.1155/2011/316067 |
| spellingShingle | Wenfa Lu Guojian Wei Wanling Pan Henry Tabel Trypanosoma congolense Infections: Induced Nitric Oxide Inhibits Parasite Growth In Vivo Journal of Parasitology Research |
| title | Trypanosoma congolense Infections: Induced Nitric Oxide Inhibits Parasite Growth In Vivo |
| title_full | Trypanosoma congolense Infections: Induced Nitric Oxide Inhibits Parasite Growth In Vivo |
| title_fullStr | Trypanosoma congolense Infections: Induced Nitric Oxide Inhibits Parasite Growth In Vivo |
| title_full_unstemmed | Trypanosoma congolense Infections: Induced Nitric Oxide Inhibits Parasite Growth In Vivo |
| title_short | Trypanosoma congolense Infections: Induced Nitric Oxide Inhibits Parasite Growth In Vivo |
| title_sort | trypanosoma congolense infections induced nitric oxide inhibits parasite growth in vivo |
| url | http://dx.doi.org/10.1155/2011/316067 |
| work_keys_str_mv | AT wenfalu trypanosomacongolenseinfectionsinducednitricoxideinhibitsparasitegrowthinvivo AT guojianwei trypanosomacongolenseinfectionsinducednitricoxideinhibitsparasitegrowthinvivo AT wanlingpan trypanosomacongolenseinfectionsinducednitricoxideinhibitsparasitegrowthinvivo AT henrytabel trypanosomacongolenseinfectionsinducednitricoxideinhibitsparasitegrowthinvivo |