OsHYPK/NatA-mediated N-terminal acetylation regulates the homeostasis of NLR immune protein to fine-tune rice immune responses and growth
Summary: Keeping nucleotide-binding leucine-rich repeat (NLR) protein at appropriate levels is critical for plant survival. Huntingtin Yeast Partner K (OsHYPK) was previously identified as a positive regulator of N-terminal acetyltransferase A (NatA) activity in rice. Here, we find that oshypk shows...
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| Language: | English |
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Elsevier
2025-05-01
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| Series: | Cell Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211124725004905 |
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| author | Yaqian Huang Xiaodi Gong Hui Shi Peiyi Wang Yundong Yuan Cuilian Kong Jie Zhou Dianxing Wu Yan Liang Yonghong Wang Jing Wang |
| author_facet | Yaqian Huang Xiaodi Gong Hui Shi Peiyi Wang Yundong Yuan Cuilian Kong Jie Zhou Dianxing Wu Yan Liang Yonghong Wang Jing Wang |
| author_sort | Yaqian Huang |
| collection | DOAJ |
| description | Summary: Keeping nucleotide-binding leucine-rich repeat (NLR) protein at appropriate levels is critical for plant survival. Huntingtin Yeast Partner K (OsHYPK) was previously identified as a positive regulator of N-terminal acetyltransferase A (NatA) activity in rice. Here, we find that oshypk shows enhanced resistance to Magnaporthe oryzae (M. oryzae). Through screening for suppressors of oshypk (soh), we identify suppressor soh74, which contains a mutation in RESISTANCE TO P. SYRINGAE PV MACULICOLA1 (RPM1)-like NLR protein (RPM1-L1) and exhibits compromised resistance to M. oryzae. Mechanistically, declined N-terminal acetylation (NTA) degree in oshypk leads to protein accumulation of RPM1-L1, contributing to enhanced disease resistance. To restrict RPM1-L1 accumulation, OsHYPK is expressed at high levels under normal conditions. However, pathogen infection reduces OsHYPK level to release the inhibition on RPM1-L1, leading to immune activation. This study reveals a vital pathway in which OsHYPK/NatA-mediated NTA rapidly fine-tunes NLR-mediated immune response. |
| format | Article |
| id | doaj-art-1ce8d5466fee4ccba319c83905381afd |
| institution | Kabale University |
| issn | 2211-1247 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cell Reports |
| spelling | doaj-art-1ce8d5466fee4ccba319c83905381afd2025-08-20T03:49:17ZengElsevierCell Reports2211-12472025-05-0144511571910.1016/j.celrep.2025.115719OsHYPK/NatA-mediated N-terminal acetylation regulates the homeostasis of NLR immune protein to fine-tune rice immune responses and growthYaqian Huang0Xiaodi Gong1Hui Shi2Peiyi Wang3Yundong Yuan4Cuilian Kong5Jie Zhou6Dianxing Wu7Yan Liang8Yonghong Wang9Jing Wang10Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China; University of Chinese Academy of Sciences, Beijing 100039, ChinaInstitute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China; University of Chinese Academy of Sciences, Beijing 100039, ChinaState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University at Wenjiang, Chengdu, Sichuan 611130, ChinaCollege of Life Sciences, Shandong Agriculture University, Tai’an, Shandong 271018, ChinaCollege of Life Sciences, Shandong Agriculture University, Tai’an, Shandong 271018, ChinaState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University at Wenjiang, Chengdu, Sichuan 611130, ChinaInstitute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, ChinaCollege of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, ChinaCollege of Life Sciences, Shandong Agriculture University, Tai’an, Shandong 271018, ChinaInstitute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China; University of Chinese Academy of Sciences, Beijing 100039, China; College of Life Sciences, Shandong Agriculture University, Tai’an, Shandong 271018, China; Corresponding authorState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University at Wenjiang, Chengdu, Sichuan 611130, China; College of Agronomy, Sichuan Agricultural University at Wenjiang, Chengdu, Sichuan 611130, China; Corresponding authorSummary: Keeping nucleotide-binding leucine-rich repeat (NLR) protein at appropriate levels is critical for plant survival. Huntingtin Yeast Partner K (OsHYPK) was previously identified as a positive regulator of N-terminal acetyltransferase A (NatA) activity in rice. Here, we find that oshypk shows enhanced resistance to Magnaporthe oryzae (M. oryzae). Through screening for suppressors of oshypk (soh), we identify suppressor soh74, which contains a mutation in RESISTANCE TO P. SYRINGAE PV MACULICOLA1 (RPM1)-like NLR protein (RPM1-L1) and exhibits compromised resistance to M. oryzae. Mechanistically, declined N-terminal acetylation (NTA) degree in oshypk leads to protein accumulation of RPM1-L1, contributing to enhanced disease resistance. To restrict RPM1-L1 accumulation, OsHYPK is expressed at high levels under normal conditions. However, pathogen infection reduces OsHYPK level to release the inhibition on RPM1-L1, leading to immune activation. This study reveals a vital pathway in which OsHYPK/NatA-mediated NTA rapidly fine-tunes NLR-mediated immune response.http://www.sciencedirect.com/science/article/pii/S2211124725004905CP: Plants |
| spellingShingle | Yaqian Huang Xiaodi Gong Hui Shi Peiyi Wang Yundong Yuan Cuilian Kong Jie Zhou Dianxing Wu Yan Liang Yonghong Wang Jing Wang OsHYPK/NatA-mediated N-terminal acetylation regulates the homeostasis of NLR immune protein to fine-tune rice immune responses and growth Cell Reports CP: Plants |
| title | OsHYPK/NatA-mediated N-terminal acetylation regulates the homeostasis of NLR immune protein to fine-tune rice immune responses and growth |
| title_full | OsHYPK/NatA-mediated N-terminal acetylation regulates the homeostasis of NLR immune protein to fine-tune rice immune responses and growth |
| title_fullStr | OsHYPK/NatA-mediated N-terminal acetylation regulates the homeostasis of NLR immune protein to fine-tune rice immune responses and growth |
| title_full_unstemmed | OsHYPK/NatA-mediated N-terminal acetylation regulates the homeostasis of NLR immune protein to fine-tune rice immune responses and growth |
| title_short | OsHYPK/NatA-mediated N-terminal acetylation regulates the homeostasis of NLR immune protein to fine-tune rice immune responses and growth |
| title_sort | oshypk nata mediated n terminal acetylation regulates the homeostasis of nlr immune protein to fine tune rice immune responses and growth |
| topic | CP: Plants |
| url | http://www.sciencedirect.com/science/article/pii/S2211124725004905 |
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