Comparison of Laboratory Diagnosis of Urinary Tract Infections Based on Leukocyte and Bacterial Parameters Using Standardized Microscopic and Flow Cytometry Methods
Background. Rapid and reliable tests are essential for the diagnostic laboratory confirmation of urinary tract infections (UTIs). Until now, UTI has been confirmed by the microbiology culture of urine, requiring at least 48-hour turnaround time (TAT), with a standardized microscopic method being wid...
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| Format: | Article |
| Language: | English |
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Wiley
2022-01-01
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| Series: | International Journal of Nephrology |
| Online Access: | http://dx.doi.org/10.1155/2022/9555121 |
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| author | Priskila Christy Hanna Evelina Sidjabat Anggia Augustasia Lumban Toruan Emmanuel Jairaj Moses Narazah Mohd Yussof Yessy Puspitasari Muhammad Robiul Fuadi null Aryati Ferdy Royland Marpaung |
| author_facet | Priskila Christy Hanna Evelina Sidjabat Anggia Augustasia Lumban Toruan Emmanuel Jairaj Moses Narazah Mohd Yussof Yessy Puspitasari Muhammad Robiul Fuadi null Aryati Ferdy Royland Marpaung |
| author_sort | Priskila Christy |
| collection | DOAJ |
| description | Background. Rapid and reliable tests are essential for the diagnostic laboratory confirmation of urinary tract infections (UTIs). Until now, UTI has been confirmed by the microbiology culture of urine, requiring at least 48-hour turnaround time (TAT), with a standardized microscopic method being widely favored. Automated urine flow cytometry, however, has recently been used to improve the rapid TAT by analyzing the urine sediment. This study therefore aimed to compare the diagnostic value of the Shih-Yung conventional microscopic and urine flow cytometry methods in the detection of leukocyte and bacterial parameters of patients with UTIs in an outpatient clinic. Methods. A cross-sectional study was conducted on a total of 100 patients. Seventy urine samples were positive for leukocytes and nitrite chemistry, and 30 were negative for both. The measurements of urine leukocytes and bacteria were compared between Sysmex UF-5000 urine flow cytometry and the Shih-Yung method. The diagnostic value was obtained from ROC analysis of urine flow cytometry and the culture. Results. A leukocyte cutoff value of 87.2/μL had a sensitivity and specificity of 98.33% and 95%, respectively, and 98.33% sensitivity and 75% specificity at a bacterial cutoff of 582.22/μL. Interestingly, our study identified strong and consistent agreement of leukocyte and bacterial parameters between urine flow cytometry and Shih-Yung (k = 0.959, p<0.001 and k = 0.939, p<0.001, respectively). Furthermore, through analyzing the dominance angle of the scattergram, a strong agreement was obtained with the culture result (k = 0.880, p<0.001). Conclusions. The Shih-Yung method showed consistent agreement with urine flow cytometry for the detection of leukocytes and bacteria. The use of certain cutoffs for bacterial and leukocyte parameters in urine flow cytometry demonstrated very good performance in detecting acquired symptomatic UTIs. |
| format | Article |
| id | doaj-art-1ce3f63d2b194b81a1fe4b09a6cc0595 |
| institution | Kabale University |
| issn | 2090-2158 |
| language | English |
| publishDate | 2022-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Nephrology |
| spelling | doaj-art-1ce3f63d2b194b81a1fe4b09a6cc05952025-08-20T03:54:51ZengWileyInternational Journal of Nephrology2090-21582022-01-01202210.1155/2022/9555121Comparison of Laboratory Diagnosis of Urinary Tract Infections Based on Leukocyte and Bacterial Parameters Using Standardized Microscopic and Flow Cytometry MethodsPriskila Christy0Hanna Evelina Sidjabat1Anggia Augustasia Lumban Toruan2Emmanuel Jairaj Moses3Narazah Mohd Yussof4Yessy Puspitasari5Muhammad Robiul Fuadi6null Aryati7Ferdy Royland Marpaung8Department of Clinical PathologyMenzies Health Institute QueenslandDepartment of UrologyDepartment of Biomedical SciencesDepartment of Biomedical SciencesDepartment of Clinical PathologyDepartment of Clinical PathologyDepartment of Clinical PathologyDepartment of Clinical PathologyBackground. Rapid and reliable tests are essential for the diagnostic laboratory confirmation of urinary tract infections (UTIs). Until now, UTI has been confirmed by the microbiology culture of urine, requiring at least 48-hour turnaround time (TAT), with a standardized microscopic method being widely favored. Automated urine flow cytometry, however, has recently been used to improve the rapid TAT by analyzing the urine sediment. This study therefore aimed to compare the diagnostic value of the Shih-Yung conventional microscopic and urine flow cytometry methods in the detection of leukocyte and bacterial parameters of patients with UTIs in an outpatient clinic. Methods. A cross-sectional study was conducted on a total of 100 patients. Seventy urine samples were positive for leukocytes and nitrite chemistry, and 30 were negative for both. The measurements of urine leukocytes and bacteria were compared between Sysmex UF-5000 urine flow cytometry and the Shih-Yung method. The diagnostic value was obtained from ROC analysis of urine flow cytometry and the culture. Results. A leukocyte cutoff value of 87.2/μL had a sensitivity and specificity of 98.33% and 95%, respectively, and 98.33% sensitivity and 75% specificity at a bacterial cutoff of 582.22/μL. Interestingly, our study identified strong and consistent agreement of leukocyte and bacterial parameters between urine flow cytometry and Shih-Yung (k = 0.959, p<0.001 and k = 0.939, p<0.001, respectively). Furthermore, through analyzing the dominance angle of the scattergram, a strong agreement was obtained with the culture result (k = 0.880, p<0.001). Conclusions. The Shih-Yung method showed consistent agreement with urine flow cytometry for the detection of leukocytes and bacteria. The use of certain cutoffs for bacterial and leukocyte parameters in urine flow cytometry demonstrated very good performance in detecting acquired symptomatic UTIs.http://dx.doi.org/10.1155/2022/9555121 |
| spellingShingle | Priskila Christy Hanna Evelina Sidjabat Anggia Augustasia Lumban Toruan Emmanuel Jairaj Moses Narazah Mohd Yussof Yessy Puspitasari Muhammad Robiul Fuadi null Aryati Ferdy Royland Marpaung Comparison of Laboratory Diagnosis of Urinary Tract Infections Based on Leukocyte and Bacterial Parameters Using Standardized Microscopic and Flow Cytometry Methods International Journal of Nephrology |
| title | Comparison of Laboratory Diagnosis of Urinary Tract Infections Based on Leukocyte and Bacterial Parameters Using Standardized Microscopic and Flow Cytometry Methods |
| title_full | Comparison of Laboratory Diagnosis of Urinary Tract Infections Based on Leukocyte and Bacterial Parameters Using Standardized Microscopic and Flow Cytometry Methods |
| title_fullStr | Comparison of Laboratory Diagnosis of Urinary Tract Infections Based on Leukocyte and Bacterial Parameters Using Standardized Microscopic and Flow Cytometry Methods |
| title_full_unstemmed | Comparison of Laboratory Diagnosis of Urinary Tract Infections Based on Leukocyte and Bacterial Parameters Using Standardized Microscopic and Flow Cytometry Methods |
| title_short | Comparison of Laboratory Diagnosis of Urinary Tract Infections Based on Leukocyte and Bacterial Parameters Using Standardized Microscopic and Flow Cytometry Methods |
| title_sort | comparison of laboratory diagnosis of urinary tract infections based on leukocyte and bacterial parameters using standardized microscopic and flow cytometry methods |
| url | http://dx.doi.org/10.1155/2022/9555121 |
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