Altered diversity and composition of gut microbiota in Korean children with food allergy
Abstract Background This study aimed to comprehensively characterize the gut microbiome and identify individual and grouped gut microbes associated with food allergy (FA) using 16S rRNA gene sequencing. Methods Fecal samples were collected from children with IgE‐mediated FA and from sex‐ and age‐mat...
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Wiley
2025-03-01
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| Series: | Clinical and Translational Allergy |
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| Online Access: | https://doi.org/10.1002/clt2.70036 |
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| author | Minyoung Jung Ji Young Lee Sukyung Kim Jeongmin Song Sehun Jang Sanghee Shin Min Hee Lee Mi Jin Kim Jiwon Kim Han Byul Lee Yeonghee Kim Kangmo Ahn Minji Kim Jihyun Kim |
| author_facet | Minyoung Jung Ji Young Lee Sukyung Kim Jeongmin Song Sehun Jang Sanghee Shin Min Hee Lee Mi Jin Kim Jiwon Kim Han Byul Lee Yeonghee Kim Kangmo Ahn Minji Kim Jihyun Kim |
| author_sort | Minyoung Jung |
| collection | DOAJ |
| description | Abstract Background This study aimed to comprehensively characterize the gut microbiome and identify individual and grouped gut microbes associated with food allergy (FA) using 16S rRNA gene sequencing. Methods Fecal samples were collected from children with IgE‐mediated FA and from sex‐ and age‐matched controls. The V3–V4 variable regions of the 16S rRNA gene of the gut microbiome were profiled using next‐generation sequencing (Illumina, USA). Bacterial species richness, intracommunity diversity, and intergroup dissimilarity were evaluated. Functional profiles were predicted using Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt) and the Minimal Set of Pathways (MinPath) algorithm. Results Fecal samples were collected from children with IgE‐mediated FA (n = 66) and from sex‐ and age‐matched controls (n = 22). Gut microbiome richness (p < 0.0001), intra‐community diversity (p < 0.0001), and inter‐community diversity (p = 0.0004) were higher in the healthy group than in the FA group. Patients with FA were enriched in Blautia, Fusicatenibacter, and Ruminococcus_g5 compared with healthy control individuals (all p < 0.05). Healthy control individuals were significantly enriched in Oscillibacter and Ruminococcus compared with patients with FA (all p < 0.05). Functional pathway analysis identified enrichment in pathways related to endoglucanase in healthy controls and the ATP‐binding cassette (ABC) transport system in FA patients. Conclusions The gut microbiomes of patients with FA and healthy control individuals had different taxonomic abundances, and the microbiome richness and diversity of the bacterial flora of patients with FA were reduced compared with controls. |
| format | Article |
| id | doaj-art-1cd837eab7b649b585deeaff7baf734a |
| institution | Kabale University |
| issn | 2045-7022 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Wiley |
| record_format | Article |
| series | Clinical and Translational Allergy |
| spelling | doaj-art-1cd837eab7b649b585deeaff7baf734a2025-08-20T03:44:00ZengWileyClinical and Translational Allergy2045-70222025-03-01153n/an/a10.1002/clt2.70036Altered diversity and composition of gut microbiota in Korean children with food allergyMinyoung Jung0Ji Young Lee1Sukyung Kim2Jeongmin Song3Sehun Jang4Sanghee Shin5Min Hee Lee6Mi Jin Kim7Jiwon Kim8Han Byul Lee9Yeonghee Kim10Kangmo Ahn11Minji Kim12Jihyun Kim13Department of Pediatrics Samsung Medical Center Sungkyunkwan University School of Medicine Seoul South KoreaDepartment of Pediatrics Hallym University Chuncheon Sacred Heart Hospital Chuncheon South KoreaDepartment of Pediatrics Samsung Medical Center Sungkyunkwan University School of Medicine Seoul South KoreaDepartment of Pediatrics Samsung Medical Center Sungkyunkwan University School of Medicine Seoul South KoreaDepartment of Pediatrics Samsung Medical Center Sungkyunkwan University School of Medicine Seoul South KoreaDepartment of Pediatrics Chung‐Ang University Hospital Chung‐Ang University College of Medicine Seoul South KoreaDepartment of Pediatrics Samsung Medical Center Sungkyunkwan University School of Medicine Seoul South KoreaDepartment of Pediatrics Samsung Medical Center Sungkyunkwan University School of Medicine Seoul South KoreaDepartment of Pediatrics Chungnam National University Sejong Hospital Sejong South KoreaResearch Institute for Future Medical Science Chungnam National University Sejong Hospital Sejong South KoreaDepartment of Pediatrics Samsung Medical Center Sungkyunkwan University School of Medicine Seoul South KoreaDepartment of Pediatrics Samsung Medical Center Sungkyunkwan University School of Medicine Seoul South KoreaDepartment of Pediatrics Chungnam National University Sejong Hospital Sejong South KoreaDepartment of Pediatrics Samsung Medical Center Sungkyunkwan University School of Medicine Seoul South KoreaAbstract Background This study aimed to comprehensively characterize the gut microbiome and identify individual and grouped gut microbes associated with food allergy (FA) using 16S rRNA gene sequencing. Methods Fecal samples were collected from children with IgE‐mediated FA and from sex‐ and age‐matched controls. The V3–V4 variable regions of the 16S rRNA gene of the gut microbiome were profiled using next‐generation sequencing (Illumina, USA). Bacterial species richness, intracommunity diversity, and intergroup dissimilarity were evaluated. Functional profiles were predicted using Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt) and the Minimal Set of Pathways (MinPath) algorithm. Results Fecal samples were collected from children with IgE‐mediated FA (n = 66) and from sex‐ and age‐matched controls (n = 22). Gut microbiome richness (p < 0.0001), intra‐community diversity (p < 0.0001), and inter‐community diversity (p = 0.0004) were higher in the healthy group than in the FA group. Patients with FA were enriched in Blautia, Fusicatenibacter, and Ruminococcus_g5 compared with healthy control individuals (all p < 0.05). Healthy control individuals were significantly enriched in Oscillibacter and Ruminococcus compared with patients with FA (all p < 0.05). Functional pathway analysis identified enrichment in pathways related to endoglucanase in healthy controls and the ATP‐binding cassette (ABC) transport system in FA patients. Conclusions The gut microbiomes of patients with FA and healthy control individuals had different taxonomic abundances, and the microbiome richness and diversity of the bacterial flora of patients with FA were reduced compared with controls.https://doi.org/10.1002/clt2.7003616S rDNA sequencingdiversityfood allergymicrobiomerichness |
| spellingShingle | Minyoung Jung Ji Young Lee Sukyung Kim Jeongmin Song Sehun Jang Sanghee Shin Min Hee Lee Mi Jin Kim Jiwon Kim Han Byul Lee Yeonghee Kim Kangmo Ahn Minji Kim Jihyun Kim Altered diversity and composition of gut microbiota in Korean children with food allergy Clinical and Translational Allergy 16S rDNA sequencing diversity food allergy microbiome richness |
| title | Altered diversity and composition of gut microbiota in Korean children with food allergy |
| title_full | Altered diversity and composition of gut microbiota in Korean children with food allergy |
| title_fullStr | Altered diversity and composition of gut microbiota in Korean children with food allergy |
| title_full_unstemmed | Altered diversity and composition of gut microbiota in Korean children with food allergy |
| title_short | Altered diversity and composition of gut microbiota in Korean children with food allergy |
| title_sort | altered diversity and composition of gut microbiota in korean children with food allergy |
| topic | 16S rDNA sequencing diversity food allergy microbiome richness |
| url | https://doi.org/10.1002/clt2.70036 |
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