p57 Suppresses the Pluripotency and Proliferation of Mouse Embryonic Stem Cells by Positively Regulating p53 Activation
Embryonic stem cells (ESCs) are pluripotent stem cells that have indefinite self-renewal capacities under appropriate culture conditions in vitro. The pluripotency maintenance and proliferation of these cells are delicately governed by the concert effect of a complex transcriptional regulatory netwo...
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| Format: | Article |
| Language: | English |
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Wiley
2021-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2021/4968649 |
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| author | Na Li Zhaoyu Du Yunxiang Li Wenjing Xu Yumei Yang Haodong Peng Tianxiang Song Qihua Qin Huining Lei Jinlian Hua |
| author_facet | Na Li Zhaoyu Du Yunxiang Li Wenjing Xu Yumei Yang Haodong Peng Tianxiang Song Qihua Qin Huining Lei Jinlian Hua |
| author_sort | Na Li |
| collection | DOAJ |
| description | Embryonic stem cells (ESCs) are pluripotent stem cells that have indefinite self-renewal capacities under appropriate culture conditions in vitro. The pluripotency maintenance and proliferation of these cells are delicately governed by the concert effect of a complex transcriptional regulatory network. Herein, we discovered that p57Kip2 (p57), a cyclin-dependent kinase inhibitor canonically inhibiting cell proliferation, played a role in suppressing the pluripotency state of mouse ESCs (mESCs). p57 knockdown significantly stimulated the expressions of core pluripotency factors NANOG, OCT4, and SOX2, while p57 overexpression inhibited the expressions of these factors in mESCs. In addition, consistent with its function in somatic cells, p57 suppressed mESC proliferation. Further analysis showed that p57 could interact with and contribute to the activation of p53 in mESCs. In conclusion, the present study showed that p57 could antagonize the pluripotency state and the proliferation process of mESCs. This finding uncovers a novel function of p57 and provides new evidence for elucidating the complex regulatory of network of mESC fate. |
| format | Article |
| id | doaj-art-1cd55df98b914ce3bfabe9c94d885c59 |
| institution | Kabale University |
| issn | 1687-9678 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-1cd55df98b914ce3bfabe9c94d885c592025-02-03T01:21:16ZengWileyStem Cells International1687-96782021-01-01202110.1155/2021/4968649p57 Suppresses the Pluripotency and Proliferation of Mouse Embryonic Stem Cells by Positively Regulating p53 ActivationNa Li0Zhaoyu Du1Yunxiang Li2Wenjing Xu3Yumei Yang4Haodong Peng5Tianxiang Song6Qihua Qin7Huining Lei8Jinlian Hua9College of Veterinary MedicineCollege of Veterinary MedicineCollege of Veterinary MedicineCollege of Veterinary MedicineCollege of Veterinary MedicineCollege of Veterinary MedicineCollege of Veterinary MedicineCollege of Veterinary MedicineCollege of Veterinary MedicineCollege of Veterinary MedicineEmbryonic stem cells (ESCs) are pluripotent stem cells that have indefinite self-renewal capacities under appropriate culture conditions in vitro. The pluripotency maintenance and proliferation of these cells are delicately governed by the concert effect of a complex transcriptional regulatory network. Herein, we discovered that p57Kip2 (p57), a cyclin-dependent kinase inhibitor canonically inhibiting cell proliferation, played a role in suppressing the pluripotency state of mouse ESCs (mESCs). p57 knockdown significantly stimulated the expressions of core pluripotency factors NANOG, OCT4, and SOX2, while p57 overexpression inhibited the expressions of these factors in mESCs. In addition, consistent with its function in somatic cells, p57 suppressed mESC proliferation. Further analysis showed that p57 could interact with and contribute to the activation of p53 in mESCs. In conclusion, the present study showed that p57 could antagonize the pluripotency state and the proliferation process of mESCs. This finding uncovers a novel function of p57 and provides new evidence for elucidating the complex regulatory of network of mESC fate.http://dx.doi.org/10.1155/2021/4968649 |
| spellingShingle | Na Li Zhaoyu Du Yunxiang Li Wenjing Xu Yumei Yang Haodong Peng Tianxiang Song Qihua Qin Huining Lei Jinlian Hua p57 Suppresses the Pluripotency and Proliferation of Mouse Embryonic Stem Cells by Positively Regulating p53 Activation Stem Cells International |
| title | p57 Suppresses the Pluripotency and Proliferation of Mouse Embryonic Stem Cells by Positively Regulating p53 Activation |
| title_full | p57 Suppresses the Pluripotency and Proliferation of Mouse Embryonic Stem Cells by Positively Regulating p53 Activation |
| title_fullStr | p57 Suppresses the Pluripotency and Proliferation of Mouse Embryonic Stem Cells by Positively Regulating p53 Activation |
| title_full_unstemmed | p57 Suppresses the Pluripotency and Proliferation of Mouse Embryonic Stem Cells by Positively Regulating p53 Activation |
| title_short | p57 Suppresses the Pluripotency and Proliferation of Mouse Embryonic Stem Cells by Positively Regulating p53 Activation |
| title_sort | p57 suppresses the pluripotency and proliferation of mouse embryonic stem cells by positively regulating p53 activation |
| url | http://dx.doi.org/10.1155/2021/4968649 |
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