Dual-functional injectable adhesive hydrogel delivering ginger-derived doxorubicin vesicles for osteosarcoma recurrence suppression and post-resection wound healing

IntroductionElderly osteosarcoma patients often face significant postoperative challenges, including high recurrence rates and delayed wound healing. These issues are primarily due to inadequate hemostasis, reactive oxygen species (ROS)-mediated microenvironmental inhibition, and compromised bone re...

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Main Authors: Qiang Zhang, Yu Zhang, Hui Chen, Lei-Na Sun, Bin Zhang, Dong-Sheng Yue, Chang-Li Wang, Zhen-Fa Zhang
Format: Article
Language:English
Published: Frontiers Media S.A. 2025-06-01
Series:Frontiers in Bioengineering and Biotechnology
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Online Access:https://www.frontiersin.org/articles/10.3389/fbioe.2025.1609673/full
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author Qiang Zhang
Qiang Zhang
Qiang Zhang
Qiang Zhang
Yu Zhang
Yu Zhang
Yu Zhang
Yu Zhang
Hui Chen
Hui Chen
Hui Chen
Hui Chen
Lei-Na Sun
Lei-Na Sun
Lei-Na Sun
Lei-Na Sun
Bin Zhang
Bin Zhang
Bin Zhang
Bin Zhang
Dong-Sheng Yue
Dong-Sheng Yue
Dong-Sheng Yue
Dong-Sheng Yue
Chang-Li Wang
Chang-Li Wang
Chang-Li Wang
Chang-Li Wang
Zhen-Fa Zhang
Zhen-Fa Zhang
Zhen-Fa Zhang
Zhen-Fa Zhang
author_facet Qiang Zhang
Qiang Zhang
Qiang Zhang
Qiang Zhang
Yu Zhang
Yu Zhang
Yu Zhang
Yu Zhang
Hui Chen
Hui Chen
Hui Chen
Hui Chen
Lei-Na Sun
Lei-Na Sun
Lei-Na Sun
Lei-Na Sun
Bin Zhang
Bin Zhang
Bin Zhang
Bin Zhang
Dong-Sheng Yue
Dong-Sheng Yue
Dong-Sheng Yue
Dong-Sheng Yue
Chang-Li Wang
Chang-Li Wang
Chang-Li Wang
Chang-Li Wang
Zhen-Fa Zhang
Zhen-Fa Zhang
Zhen-Fa Zhang
Zhen-Fa Zhang
author_sort Qiang Zhang
collection DOAJ
description IntroductionElderly osteosarcoma patients often face significant postoperative challenges, including high recurrence rates and delayed wound healing. These issues are primarily due to inadequate hemostasis, reactive oxygen species (ROS)-mediated microenvironmental inhibition, and compromised bone regeneration. This study aims to address these challenges by introducing a multifunctional adhesive hydrogel designed for synergistic therapy.MethodsThe hydrogel consists of carboxymethyl chitosan methacryloyl (CMCSMA) and tannic acid (TA), which form a dynamic, crosslinked polymer network capable of rapid tissue adhesion and adaptability to moist wound environments. The hydrogel incorporates ginger vesicles (GVs) loaded with doxorubicin (DOX), offering a dual therapeutic approach. The system facilitates hemostasis through physical barrier formation and activation of coagulation factors, while GVs and DOX provide controlled release for ROS scavenging, reduction of inflammation, and targeted tumor cell elimination.ResultsIn vitro experiments demonstrated the hydrogel’s ability to efficiently remove ROS and promote osteogenic differentiation. In a rat osteosarcoma resection model, the hydrogel significantly shortened hemostasis time compared to conventional sponges, reduced tumor recurrence, and accelerated wound healing.DiscussionThis study presents a multifunctional hydrogel that combines hemostasis, antioxidation, tissue repair, and recurrence prevention. The findings suggest that this integrated therapeutic approach holds substantial potential for clinical application in elderly osteosarcoma treatment, addressing critical postoperative challenges and improving patient outcomes.
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spelling doaj-art-1cd0e6ff685a44b585bfcc20655ebd182025-08-20T02:09:58ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852025-06-011310.3389/fbioe.2025.16096731609673Dual-functional injectable adhesive hydrogel delivering ginger-derived doxorubicin vesicles for osteosarcoma recurrence suppression and post-resection wound healingQiang Zhang0Qiang Zhang1Qiang Zhang2Qiang Zhang3Yu Zhang4Yu Zhang5Yu Zhang6Yu Zhang7Hui Chen8Hui Chen9Hui Chen10Hui Chen11Lei-Na Sun12Lei-Na Sun13Lei-Na Sun14Lei-Na Sun15Bin Zhang16Bin Zhang17Bin Zhang18Bin Zhang19Dong-Sheng Yue20Dong-Sheng Yue21Dong-Sheng Yue22Dong-Sheng Yue23Chang-Li Wang24Chang-Li Wang25Chang-Li Wang26Chang-Li Wang27Zhen-Fa Zhang28Zhen-Fa Zhang29Zhen-Fa Zhang30Zhen-Fa Zhang31Department of Lung Cancer, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Tianjin, ChinaKey Laboratory of Cancer Prevention and Therapy, Tianjin, ChinaTianjin Lung Cancer Center, Tianjin, ChinaTianjin’s Clinical Research Center for Cancer, Tianjin, ChinaDepartment of Lung Cancer, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Tianjin, ChinaKey Laboratory of Cancer Prevention and Therapy, Tianjin, ChinaTianjin Lung Cancer Center, Tianjin, ChinaTianjin’s Clinical Research Center for Cancer, Tianjin, ChinaDepartment of Lung Cancer, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Tianjin, ChinaKey Laboratory of Cancer Prevention and Therapy, Tianjin, ChinaTianjin Lung Cancer Center, Tianjin, ChinaTianjin’s Clinical Research Center for Cancer, Tianjin, ChinaKey Laboratory of Cancer Prevention and Therapy, Tianjin, ChinaTianjin Lung Cancer Center, Tianjin, ChinaDepartment of Pathology, Tianjin Medical University Cancer Institute and Hospital, Tianjin, ChinaTianjin’s Clinical Research Center for Cancer, Tianjin, ChinaDepartment of Lung Cancer, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Tianjin, ChinaKey Laboratory of Cancer Prevention and Therapy, Tianjin, ChinaTianjin Lung Cancer Center, Tianjin, ChinaTianjin’s Clinical Research Center for Cancer, Tianjin, ChinaDepartment of Lung Cancer, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Tianjin, ChinaKey Laboratory of Cancer Prevention and Therapy, Tianjin, ChinaTianjin Lung Cancer Center, Tianjin, ChinaTianjin’s Clinical Research Center for Cancer, Tianjin, ChinaDepartment of Lung Cancer, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Tianjin, ChinaKey Laboratory of Cancer Prevention and Therapy, Tianjin, ChinaTianjin Lung Cancer Center, Tianjin, ChinaTianjin’s Clinical Research Center for Cancer, Tianjin, ChinaDepartment of Lung Cancer, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Tianjin, ChinaKey Laboratory of Cancer Prevention and Therapy, Tianjin, ChinaTianjin Lung Cancer Center, Tianjin, ChinaTianjin’s Clinical Research Center for Cancer, Tianjin, ChinaIntroductionElderly osteosarcoma patients often face significant postoperative challenges, including high recurrence rates and delayed wound healing. These issues are primarily due to inadequate hemostasis, reactive oxygen species (ROS)-mediated microenvironmental inhibition, and compromised bone regeneration. This study aims to address these challenges by introducing a multifunctional adhesive hydrogel designed for synergistic therapy.MethodsThe hydrogel consists of carboxymethyl chitosan methacryloyl (CMCSMA) and tannic acid (TA), which form a dynamic, crosslinked polymer network capable of rapid tissue adhesion and adaptability to moist wound environments. The hydrogel incorporates ginger vesicles (GVs) loaded with doxorubicin (DOX), offering a dual therapeutic approach. The system facilitates hemostasis through physical barrier formation and activation of coagulation factors, while GVs and DOX provide controlled release for ROS scavenging, reduction of inflammation, and targeted tumor cell elimination.ResultsIn vitro experiments demonstrated the hydrogel’s ability to efficiently remove ROS and promote osteogenic differentiation. In a rat osteosarcoma resection model, the hydrogel significantly shortened hemostasis time compared to conventional sponges, reduced tumor recurrence, and accelerated wound healing.DiscussionThis study presents a multifunctional hydrogel that combines hemostasis, antioxidation, tissue repair, and recurrence prevention. The findings suggest that this integrated therapeutic approach holds substantial potential for clinical application in elderly osteosarcoma treatment, addressing critical postoperative challenges and improving patient outcomes.https://www.frontiersin.org/articles/10.3389/fbioe.2025.1609673/fullpostoperative osteosarcoma managementginger vesiclesadhesive hydrogeldoxorubicinantioxidation-enhanced bone regenerationtumor recurrence inhibition
spellingShingle Qiang Zhang
Qiang Zhang
Qiang Zhang
Qiang Zhang
Yu Zhang
Yu Zhang
Yu Zhang
Yu Zhang
Hui Chen
Hui Chen
Hui Chen
Hui Chen
Lei-Na Sun
Lei-Na Sun
Lei-Na Sun
Lei-Na Sun
Bin Zhang
Bin Zhang
Bin Zhang
Bin Zhang
Dong-Sheng Yue
Dong-Sheng Yue
Dong-Sheng Yue
Dong-Sheng Yue
Chang-Li Wang
Chang-Li Wang
Chang-Li Wang
Chang-Li Wang
Zhen-Fa Zhang
Zhen-Fa Zhang
Zhen-Fa Zhang
Zhen-Fa Zhang
Dual-functional injectable adhesive hydrogel delivering ginger-derived doxorubicin vesicles for osteosarcoma recurrence suppression and post-resection wound healing
Frontiers in Bioengineering and Biotechnology
postoperative osteosarcoma management
ginger vesicles
adhesive hydrogel
doxorubicin
antioxidation-enhanced bone regeneration
tumor recurrence inhibition
title Dual-functional injectable adhesive hydrogel delivering ginger-derived doxorubicin vesicles for osteosarcoma recurrence suppression and post-resection wound healing
title_full Dual-functional injectable adhesive hydrogel delivering ginger-derived doxorubicin vesicles for osteosarcoma recurrence suppression and post-resection wound healing
title_fullStr Dual-functional injectable adhesive hydrogel delivering ginger-derived doxorubicin vesicles for osteosarcoma recurrence suppression and post-resection wound healing
title_full_unstemmed Dual-functional injectable adhesive hydrogel delivering ginger-derived doxorubicin vesicles for osteosarcoma recurrence suppression and post-resection wound healing
title_short Dual-functional injectable adhesive hydrogel delivering ginger-derived doxorubicin vesicles for osteosarcoma recurrence suppression and post-resection wound healing
title_sort dual functional injectable adhesive hydrogel delivering ginger derived doxorubicin vesicles for osteosarcoma recurrence suppression and post resection wound healing
topic postoperative osteosarcoma management
ginger vesicles
adhesive hydrogel
doxorubicin
antioxidation-enhanced bone regeneration
tumor recurrence inhibition
url https://www.frontiersin.org/articles/10.3389/fbioe.2025.1609673/full
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