Disulfiram inhibits migration, invasion and epithelial-mesenchymal transition of gastric cancer cells via β-catenin/CTSB pathway
DSF is a well-known alcohol withdrawal agent that has shown considerable anticancer effects in preclinical studies. In the present study, we aimed to elucidate the mechanism by which DSF and CTSB modulate of the migration and invasion of GCs. Our results illustrate that DSF plays a pivotal role in s...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-12-01
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| Series: | Advances in Cancer Biology - Metastasis |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2667394025000127 |
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| author | XianYi Zhou JiuLin Li XiuYong Liao ChengYu Ding SaiJiao Na JiangRong Du QingHui Meng Peng Zheng Fan Sun |
| author_facet | XianYi Zhou JiuLin Li XiuYong Liao ChengYu Ding SaiJiao Na JiangRong Du QingHui Meng Peng Zheng Fan Sun |
| author_sort | XianYi Zhou |
| collection | DOAJ |
| description | DSF is a well-known alcohol withdrawal agent that has shown considerable anticancer effects in preclinical studies. In the present study, we aimed to elucidate the mechanism by which DSF and CTSB modulate of the migration and invasion of GCs. Our results illustrate that DSF plays a pivotal role in suppressing tumor migration, invasion, and EMT in GC by modulating CTSB expression. Furthermore, DSF was found to aggravate the reduction in β-catenin expression via GSK-3β, consequently inhibiting CTSB expression, which has a pronounced effect on invading gastric cancer cells as a metastasis-promoting gene. Notably, β-catenin was found to increase CTSB transcriptional activity by binding to the promoter of CTSB, leading to an increase in CTSB protein levels. Collectively, our findings elucidate the molecular mechanism by which DSF suppresses EMT, migration, and invasion in GC through the GSK-3β/β-catenin/CTSB pathway, underscoring the potential of CTSB as a therapeutic target for GC. |
| format | Article |
| id | doaj-art-1cc3ff4e9bc546d7a64fc23c1550371d |
| institution | Kabale University |
| issn | 2667-3940 |
| language | English |
| publishDate | 2025-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Advances in Cancer Biology - Metastasis |
| spelling | doaj-art-1cc3ff4e9bc546d7a64fc23c1550371d2025-08-20T03:29:10ZengElsevierAdvances in Cancer Biology - Metastasis2667-39402025-12-011510014310.1016/j.adcanc.2025.100143Disulfiram inhibits migration, invasion and epithelial-mesenchymal transition of gastric cancer cells via β-catenin/CTSB pathwayXianYi Zhou0JiuLin Li1XiuYong Liao2ChengYu Ding3SaiJiao Na4JiangRong Du5QingHui Meng6Peng Zheng7Fan Sun8College of Life Science and Health, Wuhan University of Science and Technology, Wuhan, Hubei Province, ChinaCollege of Life Science and Health, Wuhan University of Science and Technology, Wuhan, Hubei Province, ChinaQianjiang Central Hospital of Chongqing, Chongqing University Qianjiang Hospital, Chongqing, ChinaCollege of Life Science and Health, Wuhan University of Science and Technology, Wuhan, Hubei Province, ChinaCollege of Life Science and Health, Wuhan University of Science and Technology, Wuhan, Hubei Province, ChinaQianjiang Central Hospital of Chongqing, Chongqing University Qianjiang Hospital, Chongqing, ChinaQianjiang Central for Disease Control and Prevention, Chongqing, ChinaCollege of Life Science and Health, Wuhan University of Science and Technology, Wuhan, Hubei Province, China; Corresponding author.No. 2, Huangjia lake Road, Wuhan, 430065, China.College of Life Science and Health, Wuhan University of Science and Technology, Wuhan, Hubei Province, China; Corresponding author.No. 2, Huangjia lake Road, Wuhan, 430065, China.DSF is a well-known alcohol withdrawal agent that has shown considerable anticancer effects in preclinical studies. In the present study, we aimed to elucidate the mechanism by which DSF and CTSB modulate of the migration and invasion of GCs. Our results illustrate that DSF plays a pivotal role in suppressing tumor migration, invasion, and EMT in GC by modulating CTSB expression. Furthermore, DSF was found to aggravate the reduction in β-catenin expression via GSK-3β, consequently inhibiting CTSB expression, which has a pronounced effect on invading gastric cancer cells as a metastasis-promoting gene. Notably, β-catenin was found to increase CTSB transcriptional activity by binding to the promoter of CTSB, leading to an increase in CTSB protein levels. Collectively, our findings elucidate the molecular mechanism by which DSF suppresses EMT, migration, and invasion in GC through the GSK-3β/β-catenin/CTSB pathway, underscoring the potential of CTSB as a therapeutic target for GC.http://www.sciencedirect.com/science/article/pii/S2667394025000127DSFCTSBβ-cateninMigrationInvasion |
| spellingShingle | XianYi Zhou JiuLin Li XiuYong Liao ChengYu Ding SaiJiao Na JiangRong Du QingHui Meng Peng Zheng Fan Sun Disulfiram inhibits migration, invasion and epithelial-mesenchymal transition of gastric cancer cells via β-catenin/CTSB pathway Advances in Cancer Biology - Metastasis DSF CTSB β-catenin Migration Invasion |
| title | Disulfiram inhibits migration, invasion and epithelial-mesenchymal transition of gastric cancer cells via β-catenin/CTSB pathway |
| title_full | Disulfiram inhibits migration, invasion and epithelial-mesenchymal transition of gastric cancer cells via β-catenin/CTSB pathway |
| title_fullStr | Disulfiram inhibits migration, invasion and epithelial-mesenchymal transition of gastric cancer cells via β-catenin/CTSB pathway |
| title_full_unstemmed | Disulfiram inhibits migration, invasion and epithelial-mesenchymal transition of gastric cancer cells via β-catenin/CTSB pathway |
| title_short | Disulfiram inhibits migration, invasion and epithelial-mesenchymal transition of gastric cancer cells via β-catenin/CTSB pathway |
| title_sort | disulfiram inhibits migration invasion and epithelial mesenchymal transition of gastric cancer cells via β catenin ctsb pathway |
| topic | DSF CTSB β-catenin Migration Invasion |
| url | http://www.sciencedirect.com/science/article/pii/S2667394025000127 |
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