Dynamic Variation in Hippocampal Metabolism after Acute Stress Exposure: An In Vivo Proton Magnetic Resonance Spectroscopy Study at 9.4 T
An acute stress response is a complex process that activates the neuroendocrine and metabolic systems for homeostasis. A study on acute stress is important to understand how an organism adapts to stress for survival. However, most studies have focused on chronic stress, and there are few studies on...
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| Format: | Article |
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Wiley
2021-01-01
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| Series: | Journal of Spectroscopy |
| Online Access: | http://dx.doi.org/10.1155/2021/6533727 |
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| author | Yoon Ho Hwang Min-Hee Lee Chang-Soo Yun Yong-Tae Kim Hyeon-Man Baek Bong Soo Han Dong Youn Kim |
| author_facet | Yoon Ho Hwang Min-Hee Lee Chang-Soo Yun Yong-Tae Kim Hyeon-Man Baek Bong Soo Han Dong Youn Kim |
| author_sort | Yoon Ho Hwang |
| collection | DOAJ |
| description | An acute stress response is a complex process that activates the neuroendocrine and metabolic systems for homeostasis. A study on acute stress is important to understand how an organism adapts to stress for survival. However, most studies have focused on chronic stress, and there are few studies on acute stress. They have analyzed the metabolic alterations in the brain at a particular time after acute stress. This study explored the temporal variations of the brain metabolites in the hippocampus after acute restraint stress using proton magnetic resonance spectroscopy. All mice in the acute stress group were physically restrained for two hours in a 50 mL conical tube. A 9.4 T animal MRI and MRS scanner was used with point-resolved spectroscopy technique for data acquisition, which was repeated four times without interscan interval. Metabolites were quantified from the data using LCModel with a simulated basis set. Based on the change in concentration of metabolites, the data were statistically analyzed using two-way repeated-measures analysis of variance between groups and a support vector machine for all time points and Student’s t-test with FDR correction for each time point. The present study found that the differences between groups are significantly (P<0.05) presented in alanine and glutamate. The effect of time of the two metabolites significantly exists (P<0.05): the first, second, and third time points in alanine and the first and second time points in glutamate. A combination of stress-specific metabolites (alanine, glutamate, N-acetyl-aspartate) that best reflect the influence of acute stress was determined using a support vector machine. These findings may indicate the importance of the timing of analysis after acute stress and provide new insights into a deeper understanding of acute stress response, including the molecular mechanism of stress-related disorders and stress resilience or vulnerability. |
| format | Article |
| id | doaj-art-1cc3b7a210ee455f8931048fe20caf3e |
| institution | Kabale University |
| issn | 2314-4920 2314-4939 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Spectroscopy |
| spelling | doaj-art-1cc3b7a210ee455f8931048fe20caf3e2025-02-03T07:24:01ZengWileyJournal of Spectroscopy2314-49202314-49392021-01-01202110.1155/2021/65337276533727Dynamic Variation in Hippocampal Metabolism after Acute Stress Exposure: An In Vivo Proton Magnetic Resonance Spectroscopy Study at 9.4 TYoon Ho Hwang0Min-Hee Lee1Chang-Soo Yun2Yong-Tae Kim3Hyeon-Man Baek4Bong Soo Han5Dong Youn Kim6Department of Biomedical Engineering, Yonsei University, Wonju 26493, Republic of KoreaInstitute of Human Genomic Study, Korea University Ansan Hospital, Ansan 15355, Republic of KoreaDepartment of Radiation Convergence Engineering, Yonsei University, Wonju 26493, Republic of KoreaDepartment of Basic Medical Sciences, Lee Gil Ya Cancer and Diabetes Institute, Gachon University, Incheon 21999, Republic of KoreaDepartment of Basic Medical Sciences, Lee Gil Ya Cancer and Diabetes Institute, Gachon University, Incheon 21999, Republic of KoreaDepartment of Radiation Convergence Engineering, Yonsei University, Wonju 26493, Republic of KoreaDepartment of Biomedical Engineering, Yonsei University, Wonju 26493, Republic of KoreaAn acute stress response is a complex process that activates the neuroendocrine and metabolic systems for homeostasis. A study on acute stress is important to understand how an organism adapts to stress for survival. However, most studies have focused on chronic stress, and there are few studies on acute stress. They have analyzed the metabolic alterations in the brain at a particular time after acute stress. This study explored the temporal variations of the brain metabolites in the hippocampus after acute restraint stress using proton magnetic resonance spectroscopy. All mice in the acute stress group were physically restrained for two hours in a 50 mL conical tube. A 9.4 T animal MRI and MRS scanner was used with point-resolved spectroscopy technique for data acquisition, which was repeated four times without interscan interval. Metabolites were quantified from the data using LCModel with a simulated basis set. Based on the change in concentration of metabolites, the data were statistically analyzed using two-way repeated-measures analysis of variance between groups and a support vector machine for all time points and Student’s t-test with FDR correction for each time point. The present study found that the differences between groups are significantly (P<0.05) presented in alanine and glutamate. The effect of time of the two metabolites significantly exists (P<0.05): the first, second, and third time points in alanine and the first and second time points in glutamate. A combination of stress-specific metabolites (alanine, glutamate, N-acetyl-aspartate) that best reflect the influence of acute stress was determined using a support vector machine. These findings may indicate the importance of the timing of analysis after acute stress and provide new insights into a deeper understanding of acute stress response, including the molecular mechanism of stress-related disorders and stress resilience or vulnerability.http://dx.doi.org/10.1155/2021/6533727 |
| spellingShingle | Yoon Ho Hwang Min-Hee Lee Chang-Soo Yun Yong-Tae Kim Hyeon-Man Baek Bong Soo Han Dong Youn Kim Dynamic Variation in Hippocampal Metabolism after Acute Stress Exposure: An In Vivo Proton Magnetic Resonance Spectroscopy Study at 9.4 T Journal of Spectroscopy |
| title | Dynamic Variation in Hippocampal Metabolism after Acute Stress Exposure: An In Vivo Proton Magnetic Resonance Spectroscopy Study at 9.4 T |
| title_full | Dynamic Variation in Hippocampal Metabolism after Acute Stress Exposure: An In Vivo Proton Magnetic Resonance Spectroscopy Study at 9.4 T |
| title_fullStr | Dynamic Variation in Hippocampal Metabolism after Acute Stress Exposure: An In Vivo Proton Magnetic Resonance Spectroscopy Study at 9.4 T |
| title_full_unstemmed | Dynamic Variation in Hippocampal Metabolism after Acute Stress Exposure: An In Vivo Proton Magnetic Resonance Spectroscopy Study at 9.4 T |
| title_short | Dynamic Variation in Hippocampal Metabolism after Acute Stress Exposure: An In Vivo Proton Magnetic Resonance Spectroscopy Study at 9.4 T |
| title_sort | dynamic variation in hippocampal metabolism after acute stress exposure an in vivo proton magnetic resonance spectroscopy study at 9 4 t |
| url | http://dx.doi.org/10.1155/2021/6533727 |
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