Extraction of raw cellular wall of Enterobacter cloacae and study of its toxicity
The crude cell wall of E.cloacae was extracted by using Lysozyme , DNase and RNase enzymes, the contents of the proteins and carbohydrates were 24% and 1.44% respectively . The pathogenicity of the bacteria was studied by testing its lethality, in which the LD50 was identified to both...
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| Format: | Article |
| Language: | English |
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University of Baghdad, College of Science for Women
2024-10-01
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| Series: | مجلة بغداد للعلوم |
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| Online Access: | https://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/11907 |
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| author | N. Sh. M. Hussain M. T. flaih |
| author_facet | N. Sh. M. Hussain M. T. flaih |
| author_sort | N. Sh. M. Hussain |
| collection | DOAJ |
| description |
The crude cell wall of E.cloacae was extracted by using Lysozyme , DNase and RNase enzymes, the contents of the proteins and carbohydrates were 24% and 1.44% respectively .
The pathogenicity of the bacteria was studied by testing its lethality, in which the LD50 was identified to both bacterial suspension and the extract of the crude cell wall of bacteria by using white swiss female mice. The value of the LD50 of bacterial suspension was 3.16x109 CFU / ml . The cell wall extract didn’t have any lethal effect on the mice, but it had a great effect on their physiological state which led to a deficit in their weight by an average of 8 grams.
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| format | Article |
| id | doaj-art-1cc2b7a3cb89468bb1bd26fb8ea0c1af |
| institution | Kabale University |
| issn | 2078-8665 2411-7986 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | University of Baghdad, College of Science for Women |
| record_format | Article |
| series | مجلة بغداد للعلوم |
| spelling | doaj-art-1cc2b7a3cb89468bb1bd26fb8ea0c1af2025-08-20T03:34:05ZengUniversity of Baghdad, College of Science for Womenمجلة بغداد للعلوم2078-86652411-79862024-10-017110.21123/bsj.2010.11907Extraction of raw cellular wall of Enterobacter cloacae and study of its toxicityN. Sh. M. HussainM. T. flaih The crude cell wall of E.cloacae was extracted by using Lysozyme , DNase and RNase enzymes, the contents of the proteins and carbohydrates were 24% and 1.44% respectively . The pathogenicity of the bacteria was studied by testing its lethality, in which the LD50 was identified to both bacterial suspension and the extract of the crude cell wall of bacteria by using white swiss female mice. The value of the LD50 of bacterial suspension was 3.16x109 CFU / ml . The cell wall extract didn’t have any lethal effect on the mice, but it had a great effect on their physiological state which led to a deficit in their weight by an average of 8 grams. https://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/11907- |
| spellingShingle | N. Sh. M. Hussain M. T. flaih Extraction of raw cellular wall of Enterobacter cloacae and study of its toxicity مجلة بغداد للعلوم - |
| title | Extraction of raw cellular wall of Enterobacter cloacae and study of its toxicity |
| title_full | Extraction of raw cellular wall of Enterobacter cloacae and study of its toxicity |
| title_fullStr | Extraction of raw cellular wall of Enterobacter cloacae and study of its toxicity |
| title_full_unstemmed | Extraction of raw cellular wall of Enterobacter cloacae and study of its toxicity |
| title_short | Extraction of raw cellular wall of Enterobacter cloacae and study of its toxicity |
| title_sort | extraction of raw cellular wall of enterobacter cloacae and study of its toxicity |
| topic | - |
| url | https://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/11907 |
| work_keys_str_mv | AT nshmhussain extractionofrawcellularwallofenterobactercloacaeandstudyofitstoxicity AT mtflaih extractionofrawcellularwallofenterobactercloacaeandstudyofitstoxicity |