A pan-genome perspective on the evolutionary dynamics of polyphyly, virulence, and antibiotic resistance in Salmonella enterica serovar Mbandaka highlights emerging threats to public health and food safety posed by cloud gene families
Salmonella enterica serovar Mbandaka, a prevalent foodborne pathogen, poses a threat to public health but remains poorly understood. We have determined the phylogenomic tree, genetic diversity, virulence, and antimicrobial resistance (AMR) profiles on a large genomic scale to elucidate the evolution...
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Elsevier
2025-01-01
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| Series: | Current Research in Food Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2665927124002843 |
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| author | Nai-peng Kan Zhiqiu Yin Yu-feng Qiu Enhui Zheng Jianhui Chen Jianzhong Huang Yuhui Du |
| author_facet | Nai-peng Kan Zhiqiu Yin Yu-feng Qiu Enhui Zheng Jianhui Chen Jianzhong Huang Yuhui Du |
| author_sort | Nai-peng Kan |
| collection | DOAJ |
| description | Salmonella enterica serovar Mbandaka, a prevalent foodborne pathogen, poses a threat to public health but remains poorly understood. We have determined the phylogenomic tree, genetic diversity, virulence, and antimicrobial resistance (AMR) profiles on a large genomic scale to elucidate the evolutionary dynamics within the Mbandaka pan-genome. The polyphyletic nature of this serovar is characterized by two distinct phylogenetic groups and inter-serovar recombination boundaries, that potentially arising from recombination events at the H2-antigen loci. The open pan-genome exhibited a flexible gene repertoire, with numerous cloud gene families involved in virulence and AMR. Extensive gene gain and loss observed at the terminal nodes of the phylogenetic tree indicate that Mbandaka individuals have undergone frequent gene turnover. The resulting changes in virulence and AMR genes potentially pose emerging threats to public health. We explored serovar conversion due to recombination of H-antigen loci, inter-serovar divergences in gene gain and loss, prophage-mediated acquisition of virulence factors, and the role of incompatibility group plasmids in acquiring resistance determinants as key molecular mechanisms driving the pathogenicity and antibiotic resistance of Mbandaka. Our work contributes to a comprehensive understanding of the complex mechanisms of pathogenesis and the ongoing evolutionary arms race with current therapeutic approaches in serovar Mbandaka. |
| format | Article |
| id | doaj-art-1cb76906db76495f96ea2041d41efe7f |
| institution | DOAJ |
| issn | 2665-9271 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Current Research in Food Science |
| spelling | doaj-art-1cb76906db76495f96ea2041d41efe7f2025-08-20T03:20:01ZengElsevierCurrent Research in Food Science2665-92712025-01-011010095710.1016/j.crfs.2024.100957A pan-genome perspective on the evolutionary dynamics of polyphyly, virulence, and antibiotic resistance in Salmonella enterica serovar Mbandaka highlights emerging threats to public health and food safety posed by cloud gene familiesNai-peng Kan0Zhiqiu Yin1Yu-feng Qiu2Enhui Zheng3Jianhui Chen4Jianzhong Huang5Yuhui Du6National Engineering Research Center of Industrial Microbiology and Fermentation Technology, College of Life Sciences, Fujian Normal University, Fuzhou, 350117, PR China; Fujian Provincial Center for Disease Control and Prevention, Fuzhou, 350012, PR ChinaDepartment of Clinical Laboratory, The Fifth Affiliated Hospital of Guangzhou Medical University, Guangzhou, 510700, Guangdong, PR China; Corresponding author.Fujian Provincial Center for Disease Control and Prevention, Fuzhou, 350012, PR ChinaFujian Provincial Center for Disease Control and Prevention, Fuzhou, 350012, PR ChinaFujian Provincial Center for Disease Control and Prevention, Fuzhou, 350012, PR ChinaNational Engineering Research Center of Industrial Microbiology and Fermentation Technology, College of Life Sciences, Fujian Normal University, Fuzhou, 350117, PR China; Corresponding author.MOE International Joint Research Laboratory on Synthetic Biology and Medicines, School of Biology and Biological Engineering, South China University of Technology, Guangzhou, 510006, PR China; Corresponding author.Salmonella enterica serovar Mbandaka, a prevalent foodborne pathogen, poses a threat to public health but remains poorly understood. We have determined the phylogenomic tree, genetic diversity, virulence, and antimicrobial resistance (AMR) profiles on a large genomic scale to elucidate the evolutionary dynamics within the Mbandaka pan-genome. The polyphyletic nature of this serovar is characterized by two distinct phylogenetic groups and inter-serovar recombination boundaries, that potentially arising from recombination events at the H2-antigen loci. The open pan-genome exhibited a flexible gene repertoire, with numerous cloud gene families involved in virulence and AMR. Extensive gene gain and loss observed at the terminal nodes of the phylogenetic tree indicate that Mbandaka individuals have undergone frequent gene turnover. The resulting changes in virulence and AMR genes potentially pose emerging threats to public health. We explored serovar conversion due to recombination of H-antigen loci, inter-serovar divergences in gene gain and loss, prophage-mediated acquisition of virulence factors, and the role of incompatibility group plasmids in acquiring resistance determinants as key molecular mechanisms driving the pathogenicity and antibiotic resistance of Mbandaka. Our work contributes to a comprehensive understanding of the complex mechanisms of pathogenesis and the ongoing evolutionary arms race with current therapeutic approaches in serovar Mbandaka.http://www.sciencedirect.com/science/article/pii/S2665927124002843MbandakaFoodborne pathogenEvolutionary dynamicPolyphyleticVirulenceAntimicrobial resistance |
| spellingShingle | Nai-peng Kan Zhiqiu Yin Yu-feng Qiu Enhui Zheng Jianhui Chen Jianzhong Huang Yuhui Du A pan-genome perspective on the evolutionary dynamics of polyphyly, virulence, and antibiotic resistance in Salmonella enterica serovar Mbandaka highlights emerging threats to public health and food safety posed by cloud gene families Current Research in Food Science Mbandaka Foodborne pathogen Evolutionary dynamic Polyphyletic Virulence Antimicrobial resistance |
| title | A pan-genome perspective on the evolutionary dynamics of polyphyly, virulence, and antibiotic resistance in Salmonella enterica serovar Mbandaka highlights emerging threats to public health and food safety posed by cloud gene families |
| title_full | A pan-genome perspective on the evolutionary dynamics of polyphyly, virulence, and antibiotic resistance in Salmonella enterica serovar Mbandaka highlights emerging threats to public health and food safety posed by cloud gene families |
| title_fullStr | A pan-genome perspective on the evolutionary dynamics of polyphyly, virulence, and antibiotic resistance in Salmonella enterica serovar Mbandaka highlights emerging threats to public health and food safety posed by cloud gene families |
| title_full_unstemmed | A pan-genome perspective on the evolutionary dynamics of polyphyly, virulence, and antibiotic resistance in Salmonella enterica serovar Mbandaka highlights emerging threats to public health and food safety posed by cloud gene families |
| title_short | A pan-genome perspective on the evolutionary dynamics of polyphyly, virulence, and antibiotic resistance in Salmonella enterica serovar Mbandaka highlights emerging threats to public health and food safety posed by cloud gene families |
| title_sort | pan genome perspective on the evolutionary dynamics of polyphyly virulence and antibiotic resistance in salmonella enterica serovar mbandaka highlights emerging threats to public health and food safety posed by cloud gene families |
| topic | Mbandaka Foodborne pathogen Evolutionary dynamic Polyphyletic Virulence Antimicrobial resistance |
| url | http://www.sciencedirect.com/science/article/pii/S2665927124002843 |
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