Planting of nitrogen-fixing shrubs promote soil carbon sequestration by increasing mineral-associated organic fraction

Nitrogen-fixing shrubs have been planted worldwidely due to their excellent performances in soil improvement and economic benefits. However, their role and mechanisms on soil organic carbon (SOC) sequestration are unclear. In this paper, a field experiment in plantations of Caragana korshinskii and...

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Main Authors: Nan Huang, Han Yi He, Rong Fan, Xin Ying Li, Chang Ming Zhao, Jin Hua Li
Format: Article
Language:English
Published: Elsevier 2025-05-01
Series:Geoderma
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Online Access:http://www.sciencedirect.com/science/article/pii/S001670612500120X
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author Nan Huang
Han Yi He
Rong Fan
Xin Ying Li
Chang Ming Zhao
Jin Hua Li
author_facet Nan Huang
Han Yi He
Rong Fan
Xin Ying Li
Chang Ming Zhao
Jin Hua Li
author_sort Nan Huang
collection DOAJ
description Nitrogen-fixing shrubs have been planted worldwidely due to their excellent performances in soil improvement and economic benefits. However, their role and mechanisms on soil organic carbon (SOC) sequestration are unclear. In this paper, a field experiment in plantations of Caragana korshinskii and meta-analysis were conducted to investigate the effects of planting nitrogen-fixing shrubs on SOC accumulation globally, and to explore the underlying mechanisms. Field experiment demonstrated that planting of C. korshinskii enhanced mineral-associated organic carbon (MAOC) concentration and proportion, particularly in the subsoil. This was due to the nitrogen increase-induced microbial growth and accumulation of microbial necromass. The role of microorganisms in SOC fractions in soil profiles was different. Bacterial community structure affected degradation degree of lignin phenols while fungal and bacterial community co-regulated microbial necromass carbon and thus increased MAOC in topsoil. In subsoil, fungal community increased microbial necromass carbon and thus promoted MAOC whereas the role of bacteria was minimal. Results from meta-analysis confirmed that nitrogen-fixing plantations effectively promoted SOC sequestration by increasing the content and proportion of MAOC along stand age. The increase in SOC was mainly ascribed to the nitrogen-fixing shrubs-induced increases in nitrogen availability and microbial growth along stand age. In addition, the globally average proportions of MAOC were 64.7% and 80.5% in topsoil and subsoil respectively, whereas the predicted maximums were about 87.7% and 92.4% accordingly. In all, planting of nitrogen-fixing shrubs was a great way to promote SOC content and stability by increasing mineral-associated organic fraction in afforestation for carbon sequestration.
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spelling doaj-art-1cacc478379b4cd5aaafd863f83bc44b2025-08-20T03:14:01ZengElsevierGeoderma1872-62592025-05-0145711728210.1016/j.geoderma.2025.117282Planting of nitrogen-fixing shrubs promote soil carbon sequestration by increasing mineral-associated organic fractionNan Huang0Han Yi He1Rong Fan2Xin Ying Li3Chang Ming Zhao4Jin Hua Li5State Key Laboratory of Herbage Improvement and Grassland Agro-Ecosystems, College of Ecology, Lanzhou University, Lanzhou 730000, PR China; Gansu Provincial Field Scientific Observation and Research Station of Mountain Ecosystems, Lanzhou 730000, PR ChinaState Key Laboratory of Herbage Improvement and Grassland Agro-Ecosystems, College of Ecology, Lanzhou University, Lanzhou 730000, PR China; Gansu Provincial Field Scientific Observation and Research Station of Mountain Ecosystems, Lanzhou 730000, PR ChinaState Key Laboratory of Herbage Improvement and Grassland Agro-Ecosystems, College of Ecology, Lanzhou University, Lanzhou 730000, PR China; Gansu Provincial Field Scientific Observation and Research Station of Mountain Ecosystems, Lanzhou 730000, PR ChinaState Key Laboratory of Herbage Improvement and Grassland Agro-Ecosystems, College of Ecology, Lanzhou University, Lanzhou 730000, PR China; Gansu Provincial Field Scientific Observation and Research Station of Mountain Ecosystems, Lanzhou 730000, PR ChinaState Key Laboratory of Herbage Improvement and Grassland Agro-Ecosystems, College of Ecology, Lanzhou University, Lanzhou 730000, PR China; Gansu Provincial Field Scientific Observation and Research Station of Mountain Ecosystems, Lanzhou 730000, PR ChinaState Key Laboratory of Herbage Improvement and Grassland Agro-Ecosystems, College of Ecology, Lanzhou University, Lanzhou 730000, PR China; Gansu Provincial Field Scientific Observation and Research Station of Mountain Ecosystems, Lanzhou 730000, PR China; Corresponding author.Nitrogen-fixing shrubs have been planted worldwidely due to their excellent performances in soil improvement and economic benefits. However, their role and mechanisms on soil organic carbon (SOC) sequestration are unclear. In this paper, a field experiment in plantations of Caragana korshinskii and meta-analysis were conducted to investigate the effects of planting nitrogen-fixing shrubs on SOC accumulation globally, and to explore the underlying mechanisms. Field experiment demonstrated that planting of C. korshinskii enhanced mineral-associated organic carbon (MAOC) concentration and proportion, particularly in the subsoil. This was due to the nitrogen increase-induced microbial growth and accumulation of microbial necromass. The role of microorganisms in SOC fractions in soil profiles was different. Bacterial community structure affected degradation degree of lignin phenols while fungal and bacterial community co-regulated microbial necromass carbon and thus increased MAOC in topsoil. In subsoil, fungal community increased microbial necromass carbon and thus promoted MAOC whereas the role of bacteria was minimal. Results from meta-analysis confirmed that nitrogen-fixing plantations effectively promoted SOC sequestration by increasing the content and proportion of MAOC along stand age. The increase in SOC was mainly ascribed to the nitrogen-fixing shrubs-induced increases in nitrogen availability and microbial growth along stand age. In addition, the globally average proportions of MAOC were 64.7% and 80.5% in topsoil and subsoil respectively, whereas the predicted maximums were about 87.7% and 92.4% accordingly. In all, planting of nitrogen-fixing shrubs was a great way to promote SOC content and stability by increasing mineral-associated organic fraction in afforestation for carbon sequestration.http://www.sciencedirect.com/science/article/pii/S001670612500120XNitrogen-fixing shrublandsSoil organic carbonMicrobial necromass carbonMineral-associated organic carbonMicrobial community
spellingShingle Nan Huang
Han Yi He
Rong Fan
Xin Ying Li
Chang Ming Zhao
Jin Hua Li
Planting of nitrogen-fixing shrubs promote soil carbon sequestration by increasing mineral-associated organic fraction
Geoderma
Nitrogen-fixing shrublands
Soil organic carbon
Microbial necromass carbon
Mineral-associated organic carbon
Microbial community
title Planting of nitrogen-fixing shrubs promote soil carbon sequestration by increasing mineral-associated organic fraction
title_full Planting of nitrogen-fixing shrubs promote soil carbon sequestration by increasing mineral-associated organic fraction
title_fullStr Planting of nitrogen-fixing shrubs promote soil carbon sequestration by increasing mineral-associated organic fraction
title_full_unstemmed Planting of nitrogen-fixing shrubs promote soil carbon sequestration by increasing mineral-associated organic fraction
title_short Planting of nitrogen-fixing shrubs promote soil carbon sequestration by increasing mineral-associated organic fraction
title_sort planting of nitrogen fixing shrubs promote soil carbon sequestration by increasing mineral associated organic fraction
topic Nitrogen-fixing shrublands
Soil organic carbon
Microbial necromass carbon
Mineral-associated organic carbon
Microbial community
url http://www.sciencedirect.com/science/article/pii/S001670612500120X
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