Antioxidant and Anti-Inflammatory Activities of Cutlassfish Head Peptone in RAW 264.7 Macrophages
The rapid growth of the fisheries industry has resulted in numerous by-products, usually called waste, causing environmental and economic challenges. Recent advances in valorization techniques have highlighted the potential of these by-products as sources of bioactive compounds. This study aimed to...
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MDPI AG
2025-02-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/14/3/286 |
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| author | Su-Jin Lee Jeonghyeon Im Svini Dileepa Marasinghe Eunyoung Jo Minthari Sakethanika Bandara Youngdeuk Lee Jaewon Lee Gun-Hoo Park Chulhong Oh |
| author_facet | Su-Jin Lee Jeonghyeon Im Svini Dileepa Marasinghe Eunyoung Jo Minthari Sakethanika Bandara Youngdeuk Lee Jaewon Lee Gun-Hoo Park Chulhong Oh |
| author_sort | Su-Jin Lee |
| collection | DOAJ |
| description | The rapid growth of the fisheries industry has resulted in numerous by-products, usually called waste, causing environmental and economic challenges. Recent advances in valorization techniques have highlighted the potential of these by-products as sources of bioactive compounds. This study aimed to investigate the antioxidant and anti-inflammatory activities of cutlassfish <i>(Trichiurus lepturus)</i> head peptone (CP) in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages. CP exhibited significant antioxidant activity, reducing ABTS and DPPH radical scavenging activity by up to 79.66% and 64.69%, respectively, with a maximum ferric-reducing antioxidant power (FRAP) value of 224.54 μM. CP enhanced macrophage proliferation (33.3%) and significantly mitigated LPS-induced oxidative and inflammatory responses, reducing nitric oxide (NO) production (60%) and reactive oxygen species levels (49.14%). CP suppressed the expression of inflammatory mediators, including inducible nitric oxide synthase (iNOS) and cyclooxygen-ase-2, and selectively inhibited the pro-inflammatory cytokines interleukin (IL)-1β and IL-6. Western blot analysis revealed that CP inhibited the phosphorylation of mitogen-activated protein kinases, including ERK, JNK, and p38, highlighting its role in modulating upstream inflammatory signaling pathways. CP exhibited significant antioxidant effects, particularly in scavenging ABTS and DPPH radicals, as well as reducing oxidative stress markers and inflammatory responses in LPS-stimulated macrophages. These findings suggest its potential not only as a therapeutic agent for conditions related to chronic inflammation, such as cardiovascular diseases and arthritis, but also as a functional ingredient in foods and nutraceuticals aimed at alleviating inflammation-related disorders. |
| format | Article |
| id | doaj-art-1c8d4d2f94f141e0a67d42705cbe0900 |
| institution | DOAJ |
| issn | 2076-3921 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj-art-1c8d4d2f94f141e0a67d42705cbe09002025-08-20T02:42:35ZengMDPI AGAntioxidants2076-39212025-02-0114328610.3390/antiox14030286Antioxidant and Anti-Inflammatory Activities of Cutlassfish Head Peptone in RAW 264.7 MacrophagesSu-Jin Lee0Jeonghyeon Im1Svini Dileepa Marasinghe2Eunyoung Jo3Minthari Sakethanika Bandara4Youngdeuk Lee5Jaewon Lee6Gun-Hoo Park7Chulhong Oh8Jeju Bio Research Center, Korea Institute of Ocean Science and Technology (KIOST), Jeju 63349, Republic of KoreaJeju Bio Research Center, Korea Institute of Ocean Science and Technology (KIOST), Jeju 63349, Republic of KoreaJeju Bio Research Center, Korea Institute of Ocean Science and Technology (KIOST), Jeju 63349, Republic of KoreaJeju Bio Research Center, Korea Institute of Ocean Science and Technology (KIOST), Jeju 63349, Republic of KoreaJeju Bio Research Center, Korea Institute of Ocean Science and Technology (KIOST), Jeju 63349, Republic of KoreaJeju Bio Research Center, Korea Institute of Ocean Science and Technology (KIOST), Jeju 63349, Republic of KoreaJeju Bio Research Center, Korea Institute of Ocean Science and Technology (KIOST), Jeju 63349, Republic of KoreaJeju Bio Research Center, Korea Institute of Ocean Science and Technology (KIOST), Jeju 63349, Republic of KoreaJeju Bio Research Center, Korea Institute of Ocean Science and Technology (KIOST), Jeju 63349, Republic of KoreaThe rapid growth of the fisheries industry has resulted in numerous by-products, usually called waste, causing environmental and economic challenges. Recent advances in valorization techniques have highlighted the potential of these by-products as sources of bioactive compounds. This study aimed to investigate the antioxidant and anti-inflammatory activities of cutlassfish <i>(Trichiurus lepturus)</i> head peptone (CP) in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages. CP exhibited significant antioxidant activity, reducing ABTS and DPPH radical scavenging activity by up to 79.66% and 64.69%, respectively, with a maximum ferric-reducing antioxidant power (FRAP) value of 224.54 μM. CP enhanced macrophage proliferation (33.3%) and significantly mitigated LPS-induced oxidative and inflammatory responses, reducing nitric oxide (NO) production (60%) and reactive oxygen species levels (49.14%). CP suppressed the expression of inflammatory mediators, including inducible nitric oxide synthase (iNOS) and cyclooxygen-ase-2, and selectively inhibited the pro-inflammatory cytokines interleukin (IL)-1β and IL-6. Western blot analysis revealed that CP inhibited the phosphorylation of mitogen-activated protein kinases, including ERK, JNK, and p38, highlighting its role in modulating upstream inflammatory signaling pathways. CP exhibited significant antioxidant effects, particularly in scavenging ABTS and DPPH radicals, as well as reducing oxidative stress markers and inflammatory responses in LPS-stimulated macrophages. These findings suggest its potential not only as a therapeutic agent for conditions related to chronic inflammation, such as cardiovascular diseases and arthritis, but also as a functional ingredient in foods and nutraceuticals aimed at alleviating inflammation-related disorders.https://www.mdpi.com/2076-3921/14/3/286cutlassfish head peptone (CP)fishery by-productsantioxidant activityanti-inflammatoryMAPK pathway |
| spellingShingle | Su-Jin Lee Jeonghyeon Im Svini Dileepa Marasinghe Eunyoung Jo Minthari Sakethanika Bandara Youngdeuk Lee Jaewon Lee Gun-Hoo Park Chulhong Oh Antioxidant and Anti-Inflammatory Activities of Cutlassfish Head Peptone in RAW 264.7 Macrophages Antioxidants cutlassfish head peptone (CP) fishery by-products antioxidant activity anti-inflammatory MAPK pathway |
| title | Antioxidant and Anti-Inflammatory Activities of Cutlassfish Head Peptone in RAW 264.7 Macrophages |
| title_full | Antioxidant and Anti-Inflammatory Activities of Cutlassfish Head Peptone in RAW 264.7 Macrophages |
| title_fullStr | Antioxidant and Anti-Inflammatory Activities of Cutlassfish Head Peptone in RAW 264.7 Macrophages |
| title_full_unstemmed | Antioxidant and Anti-Inflammatory Activities of Cutlassfish Head Peptone in RAW 264.7 Macrophages |
| title_short | Antioxidant and Anti-Inflammatory Activities of Cutlassfish Head Peptone in RAW 264.7 Macrophages |
| title_sort | antioxidant and anti inflammatory activities of cutlassfish head peptone in raw 264 7 macrophages |
| topic | cutlassfish head peptone (CP) fishery by-products antioxidant activity anti-inflammatory MAPK pathway |
| url | https://www.mdpi.com/2076-3921/14/3/286 |
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