Host Suitability of House Fly, Musca domestica (Diptera: Muscidae), Pupae Killed by High or Low Temperature Treatment for a Parastoid, Spalangia endius (Hymenoptera: Pteromalidae)
The objective of this study was to establish a high quality progeny production system for the house fly parasitoid, Spalangia endius (Hymenoptera: Pteromalidae), by stockpiling hosts. We performed two host killing methods before host storage: (i) heat-killed by 30 min exposure to 50°C or (ii) freeze...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2012-01-01
|
| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1100/2012/214907 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849307893788049408 |
|---|---|
| author | Kohei Ogawa Katsura Ito Tatsuya Fukuda Shin-ichi Tebayashi Ryo Arakawa |
| author_facet | Kohei Ogawa Katsura Ito Tatsuya Fukuda Shin-ichi Tebayashi Ryo Arakawa |
| author_sort | Kohei Ogawa |
| collection | DOAJ |
| description | The objective of this study was to establish a high quality progeny production system for the house fly parasitoid, Spalangia endius (Hymenoptera: Pteromalidae), by stockpiling hosts. We performed two host killing methods before host storage: (i) heat-killed by 30 min exposure to 50°C or (ii) freeze-killed by 10 min exposure to −80°C. The average number of parasitoids that emerged from nonstored house fly pupae after heat- or freeze-killing was not significantly different from live pupae. When house fly pupae stored at −20°C after heat-killing were supplied to S. endius, progeny production was significantly less than live pupae. Moreover, productivity became very low when house fly pupae refrigerated at 3°C after heat- or freeze-killing were supplied to S. endius. On the other hand, when house fly pupae stored at −80°C for 1 year after heat-killing were supplied to S. endius, the average number of parasitoids that emerged was not significantly different from live pupae. The average number of parasitoids that emerged from freeze-killed hosts kept for more than 8 weeks at −80°C was significantly fewer than live pupae. Thus, this study clarified that a higher-quality host can be maintained not only by simply storing at –80°C but also by adding heat treatment before storage. |
| format | Article |
| id | doaj-art-1c8d16d217bf471e8894beca602ad82d |
| institution | Kabale University |
| issn | 1537-744X |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-1c8d16d217bf471e8894beca602ad82d2025-08-20T03:54:37ZengWileyThe Scientific World Journal1537-744X2012-01-01201210.1100/2012/214907214907Host Suitability of House Fly, Musca domestica (Diptera: Muscidae), Pupae Killed by High or Low Temperature Treatment for a Parastoid, Spalangia endius (Hymenoptera: Pteromalidae)Kohei Ogawa0Katsura Ito1Tatsuya Fukuda2Shin-ichi Tebayashi3Ryo Arakawa4Faculty of Agriculture, Kochi University, 200 Monobe-otsu, Nankoku, Kochi 783-8502, JapanFaculty of Agriculture, Kochi University, 200 Monobe-otsu, Nankoku, Kochi 783-8502, JapanFaculty of Agriculture, Kochi University, 200 Monobe-otsu, Nankoku, Kochi 783-8502, JapanFaculty of Agriculture, Kochi University, 200 Monobe-otsu, Nankoku, Kochi 783-8502, JapanFaculty of Agriculture, Kochi University, 200 Monobe-otsu, Nankoku, Kochi 783-8502, JapanThe objective of this study was to establish a high quality progeny production system for the house fly parasitoid, Spalangia endius (Hymenoptera: Pteromalidae), by stockpiling hosts. We performed two host killing methods before host storage: (i) heat-killed by 30 min exposure to 50°C or (ii) freeze-killed by 10 min exposure to −80°C. The average number of parasitoids that emerged from nonstored house fly pupae after heat- or freeze-killing was not significantly different from live pupae. When house fly pupae stored at −20°C after heat-killing were supplied to S. endius, progeny production was significantly less than live pupae. Moreover, productivity became very low when house fly pupae refrigerated at 3°C after heat- or freeze-killing were supplied to S. endius. On the other hand, when house fly pupae stored at −80°C for 1 year after heat-killing were supplied to S. endius, the average number of parasitoids that emerged was not significantly different from live pupae. The average number of parasitoids that emerged from freeze-killed hosts kept for more than 8 weeks at −80°C was significantly fewer than live pupae. Thus, this study clarified that a higher-quality host can be maintained not only by simply storing at –80°C but also by adding heat treatment before storage.http://dx.doi.org/10.1100/2012/214907 |
| spellingShingle | Kohei Ogawa Katsura Ito Tatsuya Fukuda Shin-ichi Tebayashi Ryo Arakawa Host Suitability of House Fly, Musca domestica (Diptera: Muscidae), Pupae Killed by High or Low Temperature Treatment for a Parastoid, Spalangia endius (Hymenoptera: Pteromalidae) The Scientific World Journal |
| title | Host Suitability of House Fly, Musca domestica (Diptera: Muscidae), Pupae Killed by High or Low Temperature Treatment for a Parastoid, Spalangia endius (Hymenoptera: Pteromalidae) |
| title_full | Host Suitability of House Fly, Musca domestica (Diptera: Muscidae), Pupae Killed by High or Low Temperature Treatment for a Parastoid, Spalangia endius (Hymenoptera: Pteromalidae) |
| title_fullStr | Host Suitability of House Fly, Musca domestica (Diptera: Muscidae), Pupae Killed by High or Low Temperature Treatment for a Parastoid, Spalangia endius (Hymenoptera: Pteromalidae) |
| title_full_unstemmed | Host Suitability of House Fly, Musca domestica (Diptera: Muscidae), Pupae Killed by High or Low Temperature Treatment for a Parastoid, Spalangia endius (Hymenoptera: Pteromalidae) |
| title_short | Host Suitability of House Fly, Musca domestica (Diptera: Muscidae), Pupae Killed by High or Low Temperature Treatment for a Parastoid, Spalangia endius (Hymenoptera: Pteromalidae) |
| title_sort | host suitability of house fly musca domestica diptera muscidae pupae killed by high or low temperature treatment for a parastoid spalangia endius hymenoptera pteromalidae |
| url | http://dx.doi.org/10.1100/2012/214907 |
| work_keys_str_mv | AT koheiogawa hostsuitabilityofhouseflymuscadomesticadipteramuscidaepupaekilledbyhighorlowtemperaturetreatmentforaparastoidspalangiaendiushymenopterapteromalidae AT katsuraito hostsuitabilityofhouseflymuscadomesticadipteramuscidaepupaekilledbyhighorlowtemperaturetreatmentforaparastoidspalangiaendiushymenopterapteromalidae AT tatsuyafukuda hostsuitabilityofhouseflymuscadomesticadipteramuscidaepupaekilledbyhighorlowtemperaturetreatmentforaparastoidspalangiaendiushymenopterapteromalidae AT shinichitebayashi hostsuitabilityofhouseflymuscadomesticadipteramuscidaepupaekilledbyhighorlowtemperaturetreatmentforaparastoidspalangiaendiushymenopterapteromalidae AT ryoarakawa hostsuitabilityofhouseflymuscadomesticadipteramuscidaepupaekilledbyhighorlowtemperaturetreatmentforaparastoidspalangiaendiushymenopterapteromalidae |