A Novel OsMPK6‐OsMADS47‐PPKL1/3 Module Controls Grain Shape and Yield in Rice
Abstract Grain shape is a key determinant of both grain yield and appearance quality in rice. However, the interactions among different regulatory pathways remain unclear. Here, a novel regulatory axis centered on a MADS‐box transcription factor (TF) OsMADS47 for controlling rice grain shape is repo...
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| Format: | Article |
| Language: | English |
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Wiley
2025-08-01
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| Series: | Advanced Science |
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| Online Access: | https://doi.org/10.1002/advs.202501946 |
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| author | Jingjing Fang Yan Chun Fan Zhang Tingting Guo Mengmeng Ren Jinfeng Zhao Shoujiang Yuan Wensheng Wang Yunhai Li Xueyong Li |
| author_facet | Jingjing Fang Yan Chun Fan Zhang Tingting Guo Mengmeng Ren Jinfeng Zhao Shoujiang Yuan Wensheng Wang Yunhai Li Xueyong Li |
| author_sort | Jingjing Fang |
| collection | DOAJ |
| description | Abstract Grain shape is a key determinant of both grain yield and appearance quality in rice. However, the interactions among different regulatory pathways remain unclear. Here, a novel regulatory axis centered on a MADS‐box transcription factor (TF) OsMADS47 for controlling rice grain shape is reported. OsMADS47 overexpression results in slender grains, whereas knockout leads to short and small grains. OsMADS47 acts downstream of the OsMKKK10‐OsMKK4‐OsMPK6 cascade and can be phosphorylated by OsMPK6. Phosphorylation stabilizes OsMADS47 and enhances its transcriptional repression activities on target genes GS3 and GW8, two well‐known grain shape regulators. Meanwhile, the Kelch‐repeat protein phosphatase PPKL1/3 can dephosphorylate OsMADS47, making it unstable and releasing the transcriptional repression on target genes. Knockdown of OsMPK6 or overexpression of PPKL1/3, GS3, or GW8 partially suppresses the slender‐grain phenotype of the OsMADS47 overexpression plants. The results establish an integrated regulation pathway of grain shape and prove the hub gene OsMADS47 as a potential target for breeding rice with optimized appearance quality. |
| format | Article |
| id | doaj-art-1c85cc88d73e43fa98615c209f442ec2 |
| institution | Kabale University |
| issn | 2198-3844 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advanced Science |
| spelling | doaj-art-1c85cc88d73e43fa98615c209f442ec22025-08-20T11:56:10ZengWileyAdvanced Science2198-38442025-08-011230n/an/a10.1002/advs.202501946A Novel OsMPK6‐OsMADS47‐PPKL1/3 Module Controls Grain Shape and Yield in RiceJingjing Fang0Yan Chun1Fan Zhang2Tingting Guo3Mengmeng Ren4Jinfeng Zhao5Shoujiang Yuan6Wensheng Wang7Yunhai Li8Xueyong Li9State Key Laboratory of Crop Gene Resources and Breeding National Key Facility for Crop Gene Resources and Genetic Improvement Institute of Crop Sciences Chinese Academy of Agricultural Sciences Beijing 100081 ChinaState Key Laboratory of Crop Gene Resources and Breeding National Key Facility for Crop Gene Resources and Genetic Improvement Institute of Crop Sciences Chinese Academy of Agricultural Sciences Beijing 100081 ChinaState Key Laboratory of Crop Gene Resources and Breeding National Key Facility for Crop Gene Resources and Genetic Improvement Institute of Crop Sciences Chinese Academy of Agricultural Sciences Beijing 100081 ChinaCollege of Agriculture and Biotechnology Hunan University of Humanities Science and Technology Loudi 417000 ChinaState Key Laboratory of Crop Gene Resources and Breeding National Key Facility for Crop Gene Resources and Genetic Improvement Institute of Crop Sciences Chinese Academy of Agricultural Sciences Beijing 100081 ChinaState Key Laboratory of Crop Gene Resources and Breeding National Key Facility for Crop Gene Resources and Genetic Improvement Institute of Crop Sciences Chinese Academy of Agricultural Sciences Beijing 100081 ChinaInstitute of Wetland Agriculture and Ecology Shandong Academy of Agricultural Sciences Jinan 250100 ChinaState Key Laboratory of Crop Gene Resources and Breeding National Key Facility for Crop Gene Resources and Genetic Improvement Institute of Crop Sciences Chinese Academy of Agricultural Sciences Beijing 100081 ChinaState Key Laboratory of Plant Cell and Chromosome Engineering CAS Centre for Excellence in Molecular Plant Biology Institute of Genetics and Developmental Biology The Innovative Academy of Seed Design Chinese Academy of Sciences Beijing 100101 ChinaState Key Laboratory of Crop Gene Resources and Breeding National Key Facility for Crop Gene Resources and Genetic Improvement Institute of Crop Sciences Chinese Academy of Agricultural Sciences Beijing 100081 ChinaAbstract Grain shape is a key determinant of both grain yield and appearance quality in rice. However, the interactions among different regulatory pathways remain unclear. Here, a novel regulatory axis centered on a MADS‐box transcription factor (TF) OsMADS47 for controlling rice grain shape is reported. OsMADS47 overexpression results in slender grains, whereas knockout leads to short and small grains. OsMADS47 acts downstream of the OsMKKK10‐OsMKK4‐OsMPK6 cascade and can be phosphorylated by OsMPK6. Phosphorylation stabilizes OsMADS47 and enhances its transcriptional repression activities on target genes GS3 and GW8, two well‐known grain shape regulators. Meanwhile, the Kelch‐repeat protein phosphatase PPKL1/3 can dephosphorylate OsMADS47, making it unstable and releasing the transcriptional repression on target genes. Knockdown of OsMPK6 or overexpression of PPKL1/3, GS3, or GW8 partially suppresses the slender‐grain phenotype of the OsMADS47 overexpression plants. The results establish an integrated regulation pathway of grain shape and prove the hub gene OsMADS47 as a potential target for breeding rice with optimized appearance quality.https://doi.org/10.1002/advs.202501946grain shapekelch‐repeat protein phosphataseMADS‐box transcription factors (TFs)MAPK kinaserice |
| spellingShingle | Jingjing Fang Yan Chun Fan Zhang Tingting Guo Mengmeng Ren Jinfeng Zhao Shoujiang Yuan Wensheng Wang Yunhai Li Xueyong Li A Novel OsMPK6‐OsMADS47‐PPKL1/3 Module Controls Grain Shape and Yield in Rice Advanced Science grain shape kelch‐repeat protein phosphatase MADS‐box transcription factors (TFs) MAPK kinase rice |
| title | A Novel OsMPK6‐OsMADS47‐PPKL1/3 Module Controls Grain Shape and Yield in Rice |
| title_full | A Novel OsMPK6‐OsMADS47‐PPKL1/3 Module Controls Grain Shape and Yield in Rice |
| title_fullStr | A Novel OsMPK6‐OsMADS47‐PPKL1/3 Module Controls Grain Shape and Yield in Rice |
| title_full_unstemmed | A Novel OsMPK6‐OsMADS47‐PPKL1/3 Module Controls Grain Shape and Yield in Rice |
| title_short | A Novel OsMPK6‐OsMADS47‐PPKL1/3 Module Controls Grain Shape and Yield in Rice |
| title_sort | novel osmpk6 osmads47 ppkl1 3 module controls grain shape and yield in rice |
| topic | grain shape kelch‐repeat protein phosphatase MADS‐box transcription factors (TFs) MAPK kinase rice |
| url | https://doi.org/10.1002/advs.202501946 |
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