Preparation and in vitro biological evaluation of strontium doped β-tricalcium phosphate scaffolds for alveolar bone tissue regeneration
Alveolar bone defects resulting from periodontitis or other oral tissue diseases have garnered significant attention in regenerative medicine. A range of strategies have been devised to boost the osteogenic differentiation capacity of various biomaterials. As a human trace element, strontium (Sr) pl...
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Elsevier
2025-01-01
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| author | Congrui Liu Guiyong Xiao Zichen Wang Mengchen Xu Yinchuan Wang Nan Wang Zhenlong Sun Xiaoyan Li Yixin Yin |
| author_facet | Congrui Liu Guiyong Xiao Zichen Wang Mengchen Xu Yinchuan Wang Nan Wang Zhenlong Sun Xiaoyan Li Yixin Yin |
| author_sort | Congrui Liu |
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| description | Alveolar bone defects resulting from periodontitis or other oral tissue diseases have garnered significant attention in regenerative medicine. A range of strategies have been devised to boost the osteogenic differentiation capacity of various biomaterials. As a human trace element, strontium (Sr) plays a vital role in bone remodeling. This study examines the fabricated porous β-TCP scaffolds doped with 0 at. %, 4 at. %, and 8 at. % Sr (designated as Sr0-TCP, Sr4-TCP, and Sr8-TCP respectively), utilizing a modified negative pressure injection molding process, given the chemical similarity between β-TCP and natural bone. Extensive research was conducted on the impact of Sr doping on the composition, microstructure, mechanical behavior, degradation, and biological activity of β-TCP. Materials characterization demonstrated the successful fabrication of high-purity Srx-TCP scaffolds with similar porous structures. The density enhanced with the increasing amount of Sr, achieving a compressive strength of 3.86 MPa in the Sr8-TCP scaffold, meeting the criteria for cancellous bone implantation. Confocal laser scanning microscopy analysis revealed that Srx-TCP offered favorable circumstances for cell adhesion. The alkaline phosphatase assay and mineralization staining confirmed the osteogenic differentiation potential of Srx-TCP in vitro, particularly evident in the Sr8-TCP scaffold. Overall, the experimental results indicate the remarkable biocompatibility and outstanding osteogenic differentiation ability of Srx-TCP scaffolds. This research suggests that Srx-TCP scaffolds, characterized by applicable compressive strength, hold promise as a viable candidate for alveolar bone tissue regeneration. |
| format | Article |
| id | doaj-art-1c7fc255ef7e4fa3ab1fae822d696625 |
| institution | Kabale University |
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| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
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| series | Journal of Materials Research and Technology |
| spelling | doaj-art-1c7fc255ef7e4fa3ab1fae822d6966252025-01-19T06:25:40ZengElsevierJournal of Materials Research and Technology2238-78542025-01-013415591570Preparation and in vitro biological evaluation of strontium doped β-tricalcium phosphate scaffolds for alveolar bone tissue regenerationCongrui Liu0Guiyong Xiao1Zichen Wang2Mengchen Xu3Yinchuan Wang4Nan Wang5Zhenlong Sun6Xiaoyan Li7Yixin Yin8Department of Endodontics, School and Hospital of Stomatology, Cheeloo College of Medicine, Shandong University & Shandong Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Research Center of Dental Materials and Oral Tissue Regeneration & Shandong Provincial Clinical Research Center for Oral Diseases, Jinan, Shandong, 250012, China; Science and Technology Innovation Committee of Shenzhen Municipality, Shenzhen Research Institute of Shandong University, A301 Virtual University Park in South District of Shenzhen, Shenzhen, Guangdong, 518000, ChinaSchool of Materials Science and Engineering, Shandong University, Jinan, 250061, ChinaOffice of Academic Research, Shandong Xiehe University, Jinan, 250109, Shandong province, ChinaDepartment of Endodontics, School and Hospital of Stomatology, Cheeloo College of Medicine, Shandong University & Shandong Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Research Center of Dental Materials and Oral Tissue Regeneration & Shandong Provincial Clinical Research Center for Oral Diseases, Jinan, Shandong, 250012, ChinaSchool of Radiology, Shandong First Medical University and Shandong Academy of Medical Sciences, Tai'an, 271000, ChinaNational Engineering Laboratory of Medical Implantable Devices, Key Laboratory for Medical Implantable Devices of Shandong Province, WEGO Holding Company Limited, Weihai, 264210, ChinaNational Engineering Laboratory of Medical Implantable Devices, Key Laboratory for Medical Implantable Devices of Shandong Province, WEGO Holding Company Limited, Weihai, 264210, ChinaDepartment of Endodontics, School and Hospital of Stomatology, Cheeloo College of Medicine, Shandong University & Shandong Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Research Center of Dental Materials and Oral Tissue Regeneration & Shandong Provincial Clinical Research Center for Oral Diseases, Jinan, Shandong, 250012, China; Corresponding author.lixiaoyanwhu@sdu.edu.cnOral Implantology Center, Jinan Key Medical and Health Laboratory of Oral Diseases and Tissue Regeneration, Jinan Key Laboratory of Oral Diseases and Tissue Regeneration, Shandong Provincial Key Medical and Health Laboratory of Oral Diseases and Tissue Regeneration, Shandong Provincial Key Medical and Health Discipline of Oral Medicine, Jinan Stomatological Hospital, Jinan, 250001, Shandong Province, China; National Engineering Laboratory of Medical Implantable Devices, Key Laboratory for Medical Implantable Devices of Shandong Province, WEGO Holding Company Limited, Weihai, 264210, China; School of Materials Science and Engineering, Shandong University, Jinan, 250061, China; Corresponding author. Oral Implantology Center, Jinan Key Medical and Health Laboratory of Oral Diseases and Tissue Regeneration, Jinan Key Laboratory of Oral Diseases and Tissue Regeneration, Shandong Provincial Key Medical and Health Laboratory of Oral Diseases and Tissue Regeneration, Shandong Provincial Key Medical and Health Discipline of Oral Medicine, Jinan Stamotological Hospital, Jinan, 250001, Shandong Province, China.yinyixinwhu@126.comAlveolar bone defects resulting from periodontitis or other oral tissue diseases have garnered significant attention in regenerative medicine. A range of strategies have been devised to boost the osteogenic differentiation capacity of various biomaterials. As a human trace element, strontium (Sr) plays a vital role in bone remodeling. This study examines the fabricated porous β-TCP scaffolds doped with 0 at. %, 4 at. %, and 8 at. % Sr (designated as Sr0-TCP, Sr4-TCP, and Sr8-TCP respectively), utilizing a modified negative pressure injection molding process, given the chemical similarity between β-TCP and natural bone. Extensive research was conducted on the impact of Sr doping on the composition, microstructure, mechanical behavior, degradation, and biological activity of β-TCP. Materials characterization demonstrated the successful fabrication of high-purity Srx-TCP scaffolds with similar porous structures. The density enhanced with the increasing amount of Sr, achieving a compressive strength of 3.86 MPa in the Sr8-TCP scaffold, meeting the criteria for cancellous bone implantation. Confocal laser scanning microscopy analysis revealed that Srx-TCP offered favorable circumstances for cell adhesion. The alkaline phosphatase assay and mineralization staining confirmed the osteogenic differentiation potential of Srx-TCP in vitro, particularly evident in the Sr8-TCP scaffold. Overall, the experimental results indicate the remarkable biocompatibility and outstanding osteogenic differentiation ability of Srx-TCP scaffolds. This research suggests that Srx-TCP scaffolds, characterized by applicable compressive strength, hold promise as a viable candidate for alveolar bone tissue regeneration.http://www.sciencedirect.com/science/article/pii/S2238785424029648Tricalcium phosphateStrontium dopingBioceramicsAlveolar bone regenerationOsteogenic differentiation |
| spellingShingle | Congrui Liu Guiyong Xiao Zichen Wang Mengchen Xu Yinchuan Wang Nan Wang Zhenlong Sun Xiaoyan Li Yixin Yin Preparation and in vitro biological evaluation of strontium doped β-tricalcium phosphate scaffolds for alveolar bone tissue regeneration Journal of Materials Research and Technology Tricalcium phosphate Strontium doping Bioceramics Alveolar bone regeneration Osteogenic differentiation |
| title | Preparation and in vitro biological evaluation of strontium doped β-tricalcium phosphate scaffolds for alveolar bone tissue regeneration |
| title_full | Preparation and in vitro biological evaluation of strontium doped β-tricalcium phosphate scaffolds for alveolar bone tissue regeneration |
| title_fullStr | Preparation and in vitro biological evaluation of strontium doped β-tricalcium phosphate scaffolds for alveolar bone tissue regeneration |
| title_full_unstemmed | Preparation and in vitro biological evaluation of strontium doped β-tricalcium phosphate scaffolds for alveolar bone tissue regeneration |
| title_short | Preparation and in vitro biological evaluation of strontium doped β-tricalcium phosphate scaffolds for alveolar bone tissue regeneration |
| title_sort | preparation and in vitro biological evaluation of strontium doped β tricalcium phosphate scaffolds for alveolar bone tissue regeneration |
| topic | Tricalcium phosphate Strontium doping Bioceramics Alveolar bone regeneration Osteogenic differentiation |
| url | http://www.sciencedirect.com/science/article/pii/S2238785424029648 |
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