Order among chaos: High throughput MYCroplanters can distinguish interacting drivers of host infection in a highly stochastic system.
The likelihood that a host will be susceptible to infection is influenced by the interaction of diverse biotic and abiotic factors. As a result, substantial experimental replication and scalability are required to identify the contributions of and interactions between the host, the environment, and...
Saved in:
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2025-02-01
|
| Series: | PLoS Pathogens |
| Online Access: | https://doi.org/10.1371/journal.ppat.1012894 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850144405620523008 |
|---|---|
| author | Melissa Y Chen Leah M Fulton Ivie Huang Aileen Liman Sarzana S Hossain Corri D Hamilton Siyu Song Quentin Geissmann Kayla C King Cara H Haney |
| author_facet | Melissa Y Chen Leah M Fulton Ivie Huang Aileen Liman Sarzana S Hossain Corri D Hamilton Siyu Song Quentin Geissmann Kayla C King Cara H Haney |
| author_sort | Melissa Y Chen |
| collection | DOAJ |
| description | The likelihood that a host will be susceptible to infection is influenced by the interaction of diverse biotic and abiotic factors. As a result, substantial experimental replication and scalability are required to identify the contributions of and interactions between the host, the environment, and biotic factors such as the microbiome. For example, pathogen infection success is known to vary by host genotype, bacterial strain identity and dose, and pathogen dose. Elucidating the interactions between these factors in vivo has been challenging because testing combinations of these variables quickly becomes experimentally intractable. Here, we describe a novel high throughput plant growth system (MYCroplanters) to test how multiple host, non-pathogenic bacteria, and pathogen variables predict host health. Using an Arabidopsis-Pseudomonas host-microbe model, we found that host genotype and bacterial strain order of arrival predict host susceptibility to infection, but pathogen and non-pathogenic bacterial dose can overwhelm these effects. Host susceptibility to infection is therefore driven by complex interactions between multiple factors that can both mask and compensate for each other. However, regardless of host or inoculation conditions, the ratio of pathogen to non-pathogen emerged as a consistent correlate of disease. Our results demonstrate that high-throughput tools like MYCroplanters can isolate interacting drivers of host susceptibility to disease. Increasing the scale at which we can screen drivers of disease, such as microbiome community structure, will facilitate both disease predictions and treatments for medicine and agricultural applications. |
| format | Article |
| id | doaj-art-1c79ecb6a59b455ab5c3e4a0fcda3b0e |
| institution | OA Journals |
| issn | 1553-7366 1553-7374 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Pathogens |
| spelling | doaj-art-1c79ecb6a59b455ab5c3e4a0fcda3b0e2025-08-20T02:28:22ZengPublic Library of Science (PLoS)PLoS Pathogens1553-73661553-73742025-02-01212e101289410.1371/journal.ppat.1012894Order among chaos: High throughput MYCroplanters can distinguish interacting drivers of host infection in a highly stochastic system.Melissa Y ChenLeah M FultonIvie HuangAileen LimanSarzana S HossainCorri D HamiltonSiyu SongQuentin GeissmannKayla C KingCara H HaneyThe likelihood that a host will be susceptible to infection is influenced by the interaction of diverse biotic and abiotic factors. As a result, substantial experimental replication and scalability are required to identify the contributions of and interactions between the host, the environment, and biotic factors such as the microbiome. For example, pathogen infection success is known to vary by host genotype, bacterial strain identity and dose, and pathogen dose. Elucidating the interactions between these factors in vivo has been challenging because testing combinations of these variables quickly becomes experimentally intractable. Here, we describe a novel high throughput plant growth system (MYCroplanters) to test how multiple host, non-pathogenic bacteria, and pathogen variables predict host health. Using an Arabidopsis-Pseudomonas host-microbe model, we found that host genotype and bacterial strain order of arrival predict host susceptibility to infection, but pathogen and non-pathogenic bacterial dose can overwhelm these effects. Host susceptibility to infection is therefore driven by complex interactions between multiple factors that can both mask and compensate for each other. However, regardless of host or inoculation conditions, the ratio of pathogen to non-pathogen emerged as a consistent correlate of disease. Our results demonstrate that high-throughput tools like MYCroplanters can isolate interacting drivers of host susceptibility to disease. Increasing the scale at which we can screen drivers of disease, such as microbiome community structure, will facilitate both disease predictions and treatments for medicine and agricultural applications.https://doi.org/10.1371/journal.ppat.1012894 |
| spellingShingle | Melissa Y Chen Leah M Fulton Ivie Huang Aileen Liman Sarzana S Hossain Corri D Hamilton Siyu Song Quentin Geissmann Kayla C King Cara H Haney Order among chaos: High throughput MYCroplanters can distinguish interacting drivers of host infection in a highly stochastic system. PLoS Pathogens |
| title | Order among chaos: High throughput MYCroplanters can distinguish interacting drivers of host infection in a highly stochastic system. |
| title_full | Order among chaos: High throughput MYCroplanters can distinguish interacting drivers of host infection in a highly stochastic system. |
| title_fullStr | Order among chaos: High throughput MYCroplanters can distinguish interacting drivers of host infection in a highly stochastic system. |
| title_full_unstemmed | Order among chaos: High throughput MYCroplanters can distinguish interacting drivers of host infection in a highly stochastic system. |
| title_short | Order among chaos: High throughput MYCroplanters can distinguish interacting drivers of host infection in a highly stochastic system. |
| title_sort | order among chaos high throughput mycroplanters can distinguish interacting drivers of host infection in a highly stochastic system |
| url | https://doi.org/10.1371/journal.ppat.1012894 |
| work_keys_str_mv | AT melissaychen orderamongchaoshighthroughputmycroplanterscandistinguishinteractingdriversofhostinfectioninahighlystochasticsystem AT leahmfulton orderamongchaoshighthroughputmycroplanterscandistinguishinteractingdriversofhostinfectioninahighlystochasticsystem AT iviehuang orderamongchaoshighthroughputmycroplanterscandistinguishinteractingdriversofhostinfectioninahighlystochasticsystem AT aileenliman orderamongchaoshighthroughputmycroplanterscandistinguishinteractingdriversofhostinfectioninahighlystochasticsystem AT sarzanashossain orderamongchaoshighthroughputmycroplanterscandistinguishinteractingdriversofhostinfectioninahighlystochasticsystem AT corridhamilton orderamongchaoshighthroughputmycroplanterscandistinguishinteractingdriversofhostinfectioninahighlystochasticsystem AT siyusong orderamongchaoshighthroughputmycroplanterscandistinguishinteractingdriversofhostinfectioninahighlystochasticsystem AT quentingeissmann orderamongchaoshighthroughputmycroplanterscandistinguishinteractingdriversofhostinfectioninahighlystochasticsystem AT kaylacking orderamongchaoshighthroughputmycroplanterscandistinguishinteractingdriversofhostinfectioninahighlystochasticsystem AT carahhaney orderamongchaoshighthroughputmycroplanterscandistinguishinteractingdriversofhostinfectioninahighlystochasticsystem |